scholarly journals Isolation and Serial Propagation of Porcine Epidemic Diarrhea Virus in Cell Cultures and Partial Characterization of the Isolate.

1992 ◽  
Vol 54 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Ko-ichi KUSANAGI ◽  
Hiroyoshi KUWAHARA ◽  
Tetsuo KATOH ◽  
Tetsuo NUNOYA ◽  
Yoshihisa ISHIKAWA ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Porcine Epidemic Diarrhea Virus ◽  
Partial Characterization ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Serial Propagation

scholarly journals Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 50-61 ◽  
Author(s):  
Baochao Fan ◽  
Zhengyu Yu ◽  
Fengjiao Pang ◽  
Xiangwei Xu ◽  
Baimeng Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Cdna Clone ◽  
Porcine Epidemic Diarrhea Virus ◽  
Full Length ◽  
Diarrhea Virus ◽  
Full Length Cdna ◽  
Porcine Epidemic Diarrhea

scholarly journals Molecular characterization of the spike gene of the porcine epidemic diarrhea virus in Mexico, 2013–2016

Virus Genes ◽  
2017 ◽  
Vol 54 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Rocío Lara-Romero ◽  
Luis Gómez-Núñez ◽  
José Luis Cerriteño-Sánchez ◽  
Laura Márquez-Valdelamar ◽  
Susana Mendoza-Elvira ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Epidemic Diarrhea Virus ◽  
Spike Gene ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Yu Wu ◽  
Wei Li ◽  
Qingfeng Zhou ◽  
Qunhui Li ◽  
Zhichao Xu ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Clinical Symptoms ◽  
Vero Cells ◽  
Economic Losses ◽  
Protective Effects ◽  
Diarrhea Virus ◽  
Cytopathic Effects ◽  
Porcine Epidemic Diarrhea ◽  
Piglet Survival

Abstract Background Porcine epidemic diarrhea virus (PEDV) has caused enormous economic losses to the global pig industry. Currently available PEDV vaccine strains have limited protective effects against PEDV variant strains. Methods In this study, the highly virulent epidemic virus strain CT was serially passaged in Vero cells for up to 120 generations (P120). Characterization of the different passages revealed that compared with P10 and P64, P120 had a higher viral titer and more obvious cytopathic effects, thereby demonstrating better cell adaptability. Results Pathogenicity experiments using P120 in piglets revealed significant reductions in clinical symptoms, histopathological lesions, and intestinal PEDV antigen distribution; the piglet survival rate in the P120 group was 100%. Furthermore, whole-genome sequencing identified 13 amino acid changes in P120, which might be responsible for the attenuated virulence of P120. Conclusions Thus, an attenuated strain was obtained via cell passaging and that this strain could be used in preparing attenuated vaccines.

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

2015 ◽  
Vol 160 (8) ◽  
pp. 1931-1938 ◽  
Author(s):  
Dam Thi Vui ◽  
To Long Thanh ◽  
Nguyen Tung ◽  
Anchalee Srijangwad ◽  
Thitima Tripipat ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Complete Genome ◽  
Genome Characterization ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Characterization of porcine epidemic diarrhea virus infectivity in human embryonic kidney cells

2017 ◽  
Vol 162 (8) ◽  
pp. 2415-2419 ◽  
Author(s):  
Jian Zhang ◽  
Longjun Guo ◽  
Yunfei Xu ◽  
Lijun Yang ◽  
Hongyan Shi ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Virus Infectivity ◽  
Kidney Cells ◽  
Human Embryonic Kidney ◽  
Human Embryonic Kidney Cells ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions

2016 ◽  
Vol 226 ◽  
pp. 85-92 ◽  
Author(s):  
Qinye Song ◽  
Suzanne Stone ◽  
Donna Drebes ◽  
Laura L. Greiner ◽  
Cheryl M.T. Dvorak ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Neutralizing Activity ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Isolation and characterization of a variant porcine epidemic diarrhea virus in China

2012 ◽  
Vol 9 (1) ◽  
pp. 195 ◽  
Author(s):  
Yongfei Pan ◽  
Xiaoyan Tian ◽  
Wei Li ◽  
Qingfeng Zhou ◽  
Dongdong Wang ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Isolation And Characterization ◽  
Porcine Epidemic Diarrhea
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