Human Umbilical Cord Blood Serum Promotes Growth, Proliferation as well as Differentiation of Human Bone Marrow-derived Progenitor Cells

2006 ◽  
Author(s):  
Smruti Phadnis ◽  
Mugdha Joglekar ◽  
Vijayalakshmi Venkateshan ◽  
Surendra Ghaskadbi ◽  
Anandwardhan Hardikar ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Serum ◽  
Cord Blood ◽  
Progenitor Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cord Blood Serum ◽  
Umbilical Cord Blood Serum

scholarly journals Proteomic Profiling of Human Umbilical Cord Blood Serum and its Potential Use in Regenerative Therapies

2020 ◽  
Vol 10 (2) ◽  
pp. 2167-2173
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Tissue Expression ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Proteomic Profiling ◽  
Cord Blood Serum ◽  
Umbilical Cord Blood Serum

Application of mediators directly to the injured site to promote de novo tissue can be the most promising strategy in regenerative medicine. However, understanding the mechanism of regional application of proteins /growth factors is challenging. Likewise, the proteins from Human Umbilical Cord Blood Serum(hUCBS) remain inadequately understood in terms of its therapeutic use. Several biological processes of the components in hUCBS remains unanswered. Human umbilical cord blood was collected in vacutainer tubes from 35 healthy mothers.hUCBS was obtained after centrifuge -4oC at 10,000 rpm for 20minutes. After the removal of high abundant proteins, proteomic profiling of UCBS was done by Mass Spectrometry. The Mascot search engine was used for peptide sequence identification. Proteins identified were assessed for tissue expression, cellular components, and biological functions based on Gene Ontology (GO) analyses. A total of 99 proteins were detected. The tissue expression information of proteins obtained included the Stomach, Liver, Heart, Lungs. Around 91 proteins were expressed in embryo organs. Proteins in hUBCS reflect the physiological status of the fetus/pregnancy. Major proteins found in hUCBS were expressed in the embryo, indicating that proteins were involved in organ development. Data contributed to a better understanding of the protein functions present in hUCBS.

Human Umbilical Cord Blood Serum in Culture Medium on Oocyte MaturationIn Vitro

2007 ◽  
Vol 53 (6) ◽  
pp. 303-307 ◽  
Author(s):  
Z.-G. Zhang ◽  
J.-H. Zhao ◽  
Z.-L. Wei ◽  
L. Cong ◽  
P. Zhou ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Culture Medium ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cord Blood Serum ◽  
Umbilical Cord Blood Serum

scholarly journals Proteomic Profiling of Human Umbilical Cord Blood Serum and its Potential Use in Regenerative Therapies

2020 ◽  
Vol 2 (2) ◽  
pp. 29-30
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Tissue Injury ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Differentiation Potential ◽  
Cord Blood Serum ◽  
Umbilical Cord Blood Serum

A few biomedical applications of Human umbilical cord blood serum used for the treatment of blood disorders and ocular therapy have been documented. Umbilical cords are relatively free of bacterial and viral contamination and often discarded after birth. Previous studies on human umbilical cord blood serum have certain factors exhibiting a distinctive differentiation potential and pharmacological influence like to boost anti-inflammatory, analgesic, and wound healing property. Cord blood can be collected without pain and cryopreserved without causing any changes in the functional properties. Human umbilical cord blood serum has been increasingly used in regenerative medicine because of their ability to treat tissue injury and causing immunomodulatory properties. Human cord blood serum possesses a striking natural resemblance of nutrients required for cell/tissue engineering. Cord blood serum being allogenic in nature, makes it a favorable biomaterial over other autogenous therapies. It contains a number of proteins that are exchanged from the mother to the fetus for growth and development.These exclusive proteins may perhaps participate in the modulation of cell proliferation and differentiation along with accelerated migration of keratinocyte at the wound edge with the generation of the intact epithelium.In the present study, umbilical cord blood serum was obtained from 35 patients and was subjected toMass Spectrometry. A total of 99 proteins were identified in the sample. Functions of proteins were analyzed using the Uniprot database.Thus, understanding the protein functions could give us insight into the regenerative properties of umbilical cord blood serum.

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