The effect of the bisphosphonate alendronate on viability of canine osteosarcoma cells in vitro

2004 ◽  
Author(s):  
James Farese ◽  
Jenna Ashton ◽  
Rowan Milner ◽  
Linda Lee-Ambrose ◽  
James Van Gilder
Keyword(s):  
Osteosarcoma Cells ◽  
Canine Osteosarcoma

THE EFFECT OF THE BISPHOSPHONATE ALENDRONATE ON VIABILITY OF CANINE OSTEOSARCOMA CELLS IN VITRO

2004 ◽  
Vol 40 (3) ◽  
pp. 113 ◽  
Author(s):  
JAMES P. FARESE ◽  
JENNA ASHTON ◽  
ROWAN MILNER ◽  
LINDA-LEE AMBROSE ◽  
JAMES VAN GILDER
Keyword(s):  
Osteosarcoma Cells ◽  
Canine Osteosarcoma

Muramyl Peptides Augment the In Vitro and In Vivo Cytostatic Activity of Canine Plastic-Adherent Mononuclear Cells Against Canine Osteosarcoma Cells

1993 ◽  
Vol 8 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Bernard W. Smith ◽  
Ilene D. Kurzman ◽  
Kevin T. Schultz ◽  
Charles J. Czuprynski ◽  
E. Gregory MacEwen
Keyword(s):  
Mononuclear Cells ◽  
Cytostatic Activity ◽  
Osteosarcoma Cells ◽  
Canine Osteosarcoma

In vitro retinoid-induced growth inhibition and morphologic differentiation of canine osteosarcoma cells

2000 ◽  
Vol 61 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Sung-Hyeok Hong ◽  
Tsuyoshi Kadosawa ◽  
Kazutoshi Nozaki ◽  
Manabu Mochizuki ◽  
Satoru Matsunaga ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Osteosarcoma Cells ◽  
Canine Osteosarcoma

In vitro effects of meloxicam with or without doxorubicin on canine osteosarcoma cells

2006 ◽  
Vol 29 (1) ◽  
pp. 15-23 ◽  
Author(s):  
B. WOLFESBERGER ◽  
C. HOELZL ◽  
I. WALTER ◽  
G. A. REIDER ◽  
G. FERTL ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Canine Osteosarcoma ◽  
In Vitro Effects

scholarly journals Effect of selected nonsteroidal anti-inflammatory drugs on the viability of canine osteosarcoma cells of the D-17 line: in vitro studies

2019 ◽  
Vol 63 (3) ◽  
pp. 399-403 ◽  
Author(s):  
Dominik Poradowski ◽  
Bożena Obmińska-Mrukowicz
Keyword(s):  
Effective Concentration ◽  
Tolfenamic Acid ◽  
Osteosarcoma Cells ◽  
Anticancer Effect ◽  
Canine Osteosarcoma ◽  
Half Maximal Effective Concentration ◽  
Analysis Of Results ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Abstract Introduction Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in veterinary medicine. They are used in pain control and in anti-inflammatory and antipyretic therapies. Some NSAIDs, e.g piroxicam, also have a documented anticancer effect. The objective of this study was to evaluate which of the commonly used NSAIDs (etodolac, flunixin, tolfenamic acid, carprofen, and ketoprofen) are cytotoxic to the D-17 cell line of canine osteosarcoma. Material and Methods The viability of the cells was evaluated using the MTT assay. Four independent repetitions were performed and the results are given as the average of these values; EC50 values (half maximal effective concentration) were also calculated. Results The analysis of results showed that carprofen and tolfenamic acid displayed the highest cytotoxicity. Other drugs either did not provide such effects or they were very poor. For carprofen, it was possible to determine an EC50 which fell within the limits of concentrations obtainable in canine serum after the administration of routinely used doses. Conclusion The results are promising but further studies should be conducted to confirm them, since this study is only preliminary. The possibility of introducing carprofen and tolfenamic acid into the routine treatment of osteosarcoma in dogs should be considered.

The effect of deracoxib and piroxicam on viability of canine osteosarcoma cells in vitro

2005 ◽  
Vol 3 (1) ◽  
pp. 54-55
Author(s):  
S. Royals ◽  
J. Farese ◽  
R. Milner ◽  
L. Lee-Ambrose
Keyword(s):  
Osteosarcoma Cells ◽  
Canine Osteosarcoma

scholarly journals Platelet-derived lysophosphatidic acid mediated LPAR1 activation as a therapeutic target for osteosarcoma metastasis

Oncogene ◽  
2021 ◽  
Author(s):  
Satoshi Takagi ◽  
Yuki Sasaki ◽  
Sumie Koike ◽  
Ai Takemoto ◽  
Yosuke Seto ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Pulmonary Metastasis ◽  
Lysophosphatidic Acid ◽  
Therapeutic Intervention ◽  
Molecular Mechanisms ◽  
Morphological Changes ◽  
Osteosarcoma Cells ◽  
Invasion And Metastasis ◽  
Platelet Releasate

AbstractOsteosarcoma is the most common primary malignant bone cancer, with high rates of pulmonary metastasis. Osteosarcoma patients with pulmonary metastasis have worse prognosis than those with localized disease, leading to dramatically reduced survival rates. Therefore, understanding the biological characteristics of metastatic osteosarcoma and the molecular mechanisms of invasion and metastasis of osteosarcoma cells will lead to the development of innovative therapeutic intervention for advanced osteosarcoma. Here, we identified that osteosarcoma cells commonly exhibit high platelet activation-inducing characteristics, and molecules released from activated platelets promote the invasiveness of osteosarcoma cells. Given that heat-denatured platelet releasate maintained the ability to promote osteosarcoma invasion, we focused on heat-tolerant molecules, such as lipid mediators in the platelet releasate. Osteosarcoma-induced platelet activation leads to abundant lysophosphatidic acid (LPA) release. Exposure to LPA or platelet releasate induced morphological changes and increased invasiveness of osteosarcoma cells. By analyzing publicly available transcriptome datasets and our in-house osteosarcoma patient-derived xenograft tumors, we found that LPA receptor 1 (LPAR1) is notably upregulated in osteosarcoma. LPAR1 gene KO in osteosarcoma cells abolished the platelet-mediated osteosarcoma invasion in vitro and the formation of early pulmonary metastatic foci in experimental pulmonary metastasis models. Of note, the pharmacological inhibition of LPAR1 by the orally available LPAR1 antagonist, ONO-7300243, prevented pulmonary metastasis of osteosarcoma in the mouse models. These results indicate that the LPA–LPAR1 axis is essential for the osteosarcoma invasion and metastasis, and targeting LPAR1 would be a promising therapeutic intervention for advanced osteosarcoma.

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