scholarly journals Methicillin-Resistant Staphylococcus aureus and Candida albicans Secondary Bloodstream Co-Infection in a Patient with Tubular Oesophageal Duplication

2020 ◽  
Author(s):  
Marta Carreira ◽  
Joana Pimenta
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Medical Treatment ◽  
Male Patient ◽  
Congenital Anomalies ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Adult Patients ◽  
Methicillin Resistant ◽  
Asymptomatic Adult

Gastrointestinal duplications are rare congenital anomalies usually found incidentally in asymptomatic adult patients. We report a case of methicillin-resistant Staphylococcus aureus and Candida albicans secondary bloodstream co-infection in a 57-year-old male patient with a communicating tubular oesophageal duplication. The patient completed 21 days of medical treatment with vancomycin and anidulafungin and remained well without any complications, over 2 years of follow-up.

scholarly journals Novel synthetic antimicrobial peptides effective against methicillin-resistant Staphylococcus aureus

1994 ◽  
Vol 302 (2) ◽  
pp. 535-538 ◽  
Author(s):  
J Alvarez-Bravo ◽  
S Kurata ◽  
S Natori
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Liquid Medium ◽  
Culture Medium ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antimicrobial Activities ◽  
Methicillin Resistant ◽  
Synthetic Antimicrobial Peptides

Previously, we identified a core undecapeptide of sapecin B having antimicrobial activity. Based on the structure of this peptide, we systematically synthesized peptides consisting of terminal basic motifs and internal oligo-leucine sequences and examined their antimicrobial activities. Of these peptides, RLKLLLLLRLK-NH2 and KLKLLLLLKLK-NH2 were found to have potent microbicidal activity against Staphylococcus aureus, Escherichia coli, methicillin-resistant S. aureus and Candida albicans in liquid medium. We also synthesized the D-enantiomer of KLKLLLLLKLK-NH2. This enantiomer was resistant to tryptic digestion and persisted longer in the culture medium, showing greater antimicrobial activity than the original peptide.

Trends in annual incidence of methicillin-resistant Staphylococcus aureus (MRSA) infection in HIV-infected and HIV-uninfected patients

2013 ◽  
Vol 141 (11) ◽  
pp. 2392-2402 ◽  
Author(s):  
G. N. DELORENZE ◽  
M. A. HORBERG ◽  
M. J. SILVERBERG ◽  
A. TSAI ◽  
C. P. QUESENBERRY ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Health Plan ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Incidence Rates ◽  
Infected Group ◽  
Rapid Rise ◽  
Infection Rates ◽  
Methicillin Resistant ◽  
Mrsa Infection

SUMMARYWe describe trends in incidence rates of methicillin-resistant Staphylococcus aureus (MRSA) in HIV-infected and HIV-uninfected patients enrolled in a large northern California Health Plan, and the ratio of MRSA to methicillin-susceptible S. aureus (MSSA) case counts. Between 1995 and 2010, 1549 MRSA infections were diagnosed in 14060 HIV-infected patients (11·0%) compared to 89546 MRSA infections in 6597396 HIV-uninfected patients (1·4%) (P = 0·00). A steady rise in MRSA infection rates began in 1995 in HIV-uninfected patients, peaking at 396·5 infections/100000 person-years in 2007. A more rapid rise in MRSA infection rates occurred in the HIV-infected group after 2000, peaking at 3592·8 infections/100000 in 2005. A declining trend in MRSA rates may have begun in 2008–2009. Comparing the ratio of MRSA to MSSA case counts, we observed that HIV-infected patients shouldered a greater burden of MRSA infection during most years of study follow-up compared to HIV-uninfected patients.

scholarly journals The Antimicrobial Activity of Pechuel-Loeschea leubnitziae Leaf Extract and its Effect on The Expression Level of Methicillin Resistant Staphylococcus Aureus and Candida albicans Virulence-Associated Genes

2022 ◽  
Author(s):  
Mariah Ndilimeke Muhongo ◽  
Mourine Kangogo ◽  
Christine Bii
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Leaf Extract ◽  
Target Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
Expression Level ◽  
Methicillin Resistant

The complete halt in the synthesis of new effective antimicrobial compounds is a global concern. Pathogenic microorganisms' virulence mechanisms seem to have a significant impact on their pathogenesis. The purpose of this study was to examine the antimicrobial activity of the ethanol and methanol fractions of Pechuel-Loeschea leubnitziae leaf extract, as well as its effect on the expression level of virulence-associated genes.The extract's fractions were evaluated for antimicrobial activity against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae (clinical), Methicillin-resistant Staphylococcus aureus, and Candida albicans ATCC 90029. The test organism's antibiogram pattern was determined. The extracts' attenuation effect on the target genes of the susceptible organisms was investigated employing relative quantification using RT-qPCR. The test organism's antibiogram pattern revealed that it was drug-resistant, intermediate, and sensitive. The extracts tested positive for antimicrobial activity against Methicillin-resistant Staphylococcus aureus and Candida albicans ATCC 90029, with zones of inhibition varying from 20.33 to 29 mm. The lowest recorded MIC value was 4.688 mg/ml, while the highest was 37.5 mg/ml. In contrast to the methanol extract, the ethanol extract had a cidal action at a lower dose. The ethanol extract's Sub-MIC (18.25 mg/ml) merely reduced the expression of the hly gene in MRSA. The MRSA virulence genes were not suppressed by the sub-MIC of methanol extract (18.25 mg/ml). Notably, the expression of als1, pbl1, and sap1 in Candida albicans ATCC 90029 was significantly attenuated when exposed to sub-MICs of ethanol extract (2,344 mg/ml) and methanol extract (9.375 mg/ml). Per the findings of this research, the leaves of P. leubnitziae could be a source of an effective antimicrobial agent in the therapy of MRSA/Candida-related disorders.

scholarly journals Aktivitas Antimikroba Fraksi Etil Asetat Daun Ceremai [Phyllanthus acidus (L.) Skeels] terhadap Bakteri Resisten Antimikroba dan Jamur dengan Metode KLT Bioautografi

2020 ◽  
Vol 4 ◽  
Author(s):  
Lanny Mulqie ◽  
Kusnandar Anggadireja
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Coagulase Negative Staphylococcus ◽  
Microsporum Gypseum ◽  
Vancomycin Resistant Enterococcus ◽  
Methicillin Resistant ◽  
Phyllanthus Acidus ◽  
Vancomycin Resistant

Resistensi dan toksisitas antimikroba menyebabkan penggunaan obat herbal sebagai alternatif  pengobatan berbagai penyakit yang disebabkan oleh mikroba. Ekstrak etanol daun ceremai memiliki aktivitas terhadap VRE, MRCNS, dan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba fraksi etil asetat daun ceremai terhadap bakteri resisten antimikroba [Methicillin-Resistant Staphylococcus aureus (MRSA), Methicillin-Resistant Coagulase Negative Staphylococcus (MRCNS), Vancomycin Resistant Enterococcus (VRE)] dan jamur uji (Candida albicans, Microsporum gypseum, dan Aspergillus niger) dengan metode KLT bioautografi. Fraksinasi dilakukan dengan cara ekstraksi cair-cair menggunakan pelarut dengan tingkat kepolaran yang berbeda, yaitu n-heksan, etil asetat, dan air. KLT bioautografi pada fraksi etil asetat daun ceremai dilakukan menggunakan fase diam silika gel GF254 dan pengembang etil asetat-asam format-asam asetat-air (100:10:10:22), menggunakan sitroborat sebagai penampak bercak. Pada hasil kromatogram terdapat 5 noda yang berfluoresensi hijau kekuningan dengan nilai rf noda 1 sebesar 0,35; noda 2 sebesar 0,43, noda 3 sebesar 0,62, noda 4 sebesar 0,75 dan noda 5 sebesar 0,90 setelah plat disemprot oleh sitroborat dan dilihat di bawah sinar UV. Hasil pengujian aktivitas antimikroba dengan metode KLT bioautografi menunjukkan terbentuknya zona bening yang dihasilkan noda pada plat yang ditempelkan pada media. Fraksi etil asetat daun ceremai memiliki aktivitas antimikroba terhadap bakteri resisten antimikroba (MRSA, MRCNS, dan VRE) dan jamur  uji (Candida albicans, Microsporum gypseum, dan Aspergillus niger) yang dihasilkan oleh noda 1 (rf 0,35), noda 2 (rf 0,43), noda 3 (rf 0,62), noda 4 (rf 0,75), dan noda 5 (rf 0,90). Golongan senyawa yang diduga memiliki aktivitas antimikroba adalah flavonoid.

scholarly journals Estratégia para Controlar o Staphylococcus Aureus Resistente à Meticilina: A Experiência de Cinco Anos de um Hospital

2014 ◽  
Vol 27 (1) ◽  
pp. 67 ◽  
Author(s):  
David Peres ◽  
Isabel Neves ◽  
Fernanda Vieira ◽  
Ilda Devesa
Keyword(s):  
Staphylococcus Aureus ◽  
Health Professionals ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
National Level ◽  
Intermediate Care ◽  
Methicillin Resistant ◽  
Multimodal Strategy ◽  
History Of ◽  
Multistep Procedure

<strong>Introduction:</strong> Methicillin-resistant Staphylococcus aureus remains one of the principal resistant pathogens causing serious healthcareassociated infections. The objective of this study was to control and monitor methicillin-resistant Staphylococcus aureus cases using multimodal strategy in a Portuguese hospital.<br />Material and Methods: Multistep procedure involving isolation measures and active surveillance cultures in a selected population (patients from other hospitals and nursing homes; history of hospitalization/ methicillin-resistant Staphylococcus aureus; intensive and intermediate care patients and, in other inpatient services, direct contacts of newly detected methicillin-resistant Staphylococcus aureus patients and, since 2012, patients doing hemodialysis). Other parallel activities: (a) review of isolation and standard precautions policy, (b) reinforcement of alcohol-based handrubs at point of patient care, (c) information sessions to health professionals, (d) targeted information flyer for health professionals, (e) information leaflet for patients/ visitors; (f) procedure monitoring by audit (g) patient decolonization in intensive and intermediate care units, with follow-up screenings.<br /><strong>Results:</strong> Between 2007 and 2012, methicillin-resistant Staphylococcus aureus surveillance detected a decrease in proportion from 66% to 57% and, in density of incidence, from 1.80 to 0.68 cases per thousand days of hospitalization (p &lt; 0.001; RR 0.38; CI95%: 0.29-0.49).<br /><strong>Discussion/Conclusion:</strong> According to published European data, using blood and cerebrospinal fluid isolates only, Portugal was the country with the highest level of methicillin-resistant Staphylococcus aureus in 2011. Using this inclusion criteria, our hospital reveled a proportion of methicillin-resistant Staphylococcus aureus below its national level (34% versus 55%) in 2011. Fighting methicillinresistant Staphylococcus aureus using a multimodal strategy is being effective in a high endemic level hospital, but perseverance is needed through continuous surveillance of cases, feed-back to professionals and procedure audits.

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