scholarly journals Regulation of functional cytodifferentiation and histogenesis in mammary epithelial cells: role of the extracellular matrix.

1989 ◽  
Vol 80 ◽  
pp. 61-70 ◽  
Author(s):  
M J Bissell ◽  
T G Ram
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial

Role of extracellular matrix and prolactin in functional differentiation of bovine BME-UV1 mammary epithelial cells

2011 ◽  
Vol 14 (3) ◽  
pp. 433-442 ◽  
Author(s):  
M. Kozłowski ◽  
J. Wilczak ◽  
T. Motyl ◽  
M. Gajewska
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Culture System ◽  
Mammary Epithelial Cells ◽  
3D Culture ◽  
Milk Proteins ◽  
Functional Differentiation ◽  
Mammary Epithelial

Role of extracellular matrix and prolactin in functional differentiation of bovine BME-UV1 mammary epithelial cells Interactions between extracellular matrix (ECM) and epithelial cells are necessary for proper organisation and function of the epithelium. In the present study we show that bovine mammary epithelial cell line BME-UV1 cultured on ECM components, commercially available as Matrigel™, constitutes a good model for studying mechanisms controlling functional differentiation of the bovine mammary gland. In contact with Matrigel BME-UV1 cells induce apicobasal polarity, and within 16 days form three dimensional (3D) acinar structures with a centrally localized hollow lumen, which structurally resemble mammary alveoli present in the functionally active mammary gland. We have shown that the 3D culture system enables a high expression and proper localisation of integrin receptors and tight junction proteins in BME-UV1 cells to be induced. This effect was not obtained in cells grown in the classical 2D culture system on plastic. Moreover, ECM highly stimulated the synthesis of one of the major milk proteins, β-casein, even in the absence of prolactin. Our results show that contact with ECM plays an important role in the lactogenic activity of bovine MECs, however, prolactin is necessary for the efficient secretion of milk proteins.

Effect of the extracellular matrix on plasminogen activator isozyme activities of human mammary epithelial cells in culture

1983 ◽  
Vol 3 (6) ◽  
pp. 982-990
Author(s):  
N S Yang ◽  
C Park ◽  
C Longley ◽  
P Furmanski
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Collagen Gels ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial ◽  
Normal Human ◽  
Mcf 7

Multiple molecular forms of plasminogen activator were detected in normal human mammary epithelial cells in culture. Cells derived from (normal) breast mammoplasty specimens and grown on the surface of collagen gels exhibited three major classes of plasminogen activator isozymes (Mr = 100,000 [100K], 75,000 [75K], and 55,000 [55K]). The activity of the 100K and 75K isozymes was greatly reduced when the cells were grown on conventional tissue-culture-grade plastic surfaces. MCF-7, a human mammary carcinoma cell line, exhibited predominantly or exclusively the 55K isozyme, irrespective of the cell growth substratum. The activity of the 55K isozyme was more than twofold higher for MCF-7 cells grown on collagen gels than for cells grown on plastic. Progesterone, diethylstilbestrol, and estrogen stimulated the activity of the 55K isozyme of MCF-7 cells, but only when the cells were grown on a plastic surface. The plasminogen activator activities of the normal human mammary epithelial cells were not stimulated by these hormones, irrespective of the growth substratum. These results show that the expression of plasminogen activator isozymes by human mammary epithelial cells is subject to modulation by the extracellular matrix. Normal and malignant cells may differ in their responsiveness to these effects.

Cytotoxic effects of cadmium in mammary epithelial cells: Protective role of the macrocycle [15]pyN5

2012 ◽  
Vol 50 (6) ◽  
pp. 2180-2187 ◽  
Author(s):  
Sandrina Gonçalves ◽  
Ana Sofia Fernandes ◽  
Nuno G. Oliveira ◽  
Joana Marques ◽  
Judite Costa ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Protective Role ◽  
Mammary Epithelial ◽  
Cytotoxic Effects

Extracellular Matrix Dependent Gene Regulation in Mammary Epithelial Cells

1995 ◽  
pp. 107-119 ◽  
Author(s):  
Christian Schmidhauser ◽  
Connie A. Myers ◽  
Romina Mossi ◽  
Gerald F. Casperson ◽  
Mina J. Bissell
Keyword(s):  
Extracellular Matrix ◽  
Gene Regulation ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial
Sign in / Sign up

Export Citation Format

Share Document