scholarly journals Performance in Omics Analyses of Blood Samples in Long-Term Storage: Opportunities for the Exploitation of Existing Biobanks in Environmental Health Research

2013 ◽  
Vol 121 (4) ◽  
pp. 480-487 ◽  
Author(s):  
Dennie G.A.J. Hebels ◽  
Panagiotis Georgiadis ◽  
Hector C. Keun ◽  
Toby J. Athersuch ◽  
Paolo Vineis ◽  
...  
Keyword(s):  
Environmental Health ◽  
Health Research ◽  
Blood Samples ◽  
Long Term Storage ◽  
Environmental Health Research ◽  
Term Storage

Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR

2005 ◽  
Vol 19 (3) ◽  
pp. 169-172 ◽  
Author(s):  
P. Kanjanavas ◽  
P. Tan-ariya ◽  
P. Khawsak ◽  
A. Pakpitcharoen ◽  
S. Phantana ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Wuchereria Bancrofti ◽  
Blood Samples ◽  
Long Term Storage ◽  
Term Storage

Long-term storage of blood samples at freezing temperatures in the presence of a cryoprotectant for hemiglobin assay

1987 ◽  
Vol 37 (2) ◽  
pp. 190-196 ◽  
Author(s):  
Keizo Sato ◽  
Yoshinao Katsumata ◽  
Minoru Aoki ◽  
Keiji Tamaki ◽  
Shoichi Yada
Keyword(s):  
Blood Samples ◽  
Long Term Storage ◽  
Freezing Temperatures ◽  
Term Storage

Long-term storage of blood samples as whole blood at extremely low temperatures for methemoglobin determination

1988 ◽  
Vol 37 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Keizo Sato ◽  
Keiji Tamaki ◽  
Hiroshi Okajima ◽  
Yoshinao Katsumata
Keyword(s):  
Whole Blood ◽  
Low Temperatures ◽  
Blood Samples ◽  
Long Term Storage ◽  
Term Storage

Effects of long-term storage of filter paper blood samples on neonatal thyroid stimulating hormone, thyroxin and 17-alpha-hydroxyprogesterone measurements

2008 ◽  
Vol 15 (3) ◽  
pp. 109-111 ◽  
Author(s):  
Valeria S Lando ◽  
Marcelo C Batista ◽  
Izabel T Nakamura ◽  
Cassia R Mazi ◽  
Berenice B Mendonca ◽  
...  
Keyword(s):  
Filter Paper ◽  
Thyroid Stimulating Hormone ◽  
Blood Samples ◽  
Long Term Storage ◽  
Term Storage ◽  
Stimulating Hormone

scholarly journals Shelf life of whole blood samples in a biobank and the yield of deoxyribonucleic acid during genetic testing

2020 ◽  
Vol 19 (6) ◽  
pp. 2726
Author(s):  
O. P. Skirko ◽  
A. N. Meshkov ◽  
I. A. Efimova ◽  
V. A. Kutsenko ◽  
A. V. Kiseleva ◽  
...  
Keyword(s):  
Shelf Life ◽  
Whole Blood ◽  
Deoxyribonucleic Acid ◽  
Blood Samples ◽  
Dna Concentration ◽  
National Medical ◽  
Long Term Storage ◽  
Whole Blood Samples ◽  
Term Storage

Aim. To study the effect of the shelf life of frozen whole blood samples in a biobank on the amount of released deoxyribonucleic acid (DNA).Material and methods. The study included whole blood samples placed in tubes with the anticoagulant EDTA (ethylenediaminetetraacetic acid at a concentration of 1,8 mg/ml) from participants in the epidemiological study ESSE-RF-1 and ESSE-RF-2 and cohort studies conducted at the National Medical Research Center for Therapy and Preventive Medicine. The samples were stored in the biobank of the National Medical Research Center for Therapy and Preventive Medicine at temperature from -22О C to -32О C. The shelf life from blood collection to DNA extraction ranged from several weeks to 11 years. DNA was extracted using QIAamp DNA Blood Mini Kit (250) and 96 Blood Kit (Qiagen, Germany). Statistical analysis was performed using the R 3.6.1 software. To analyze the association of blood storage time with the logarithm of DNA concentration, a linear regression was used.Results. The analysis included data on the DNA concentration of 5405 samples. Multivariate regression showed that the blood shelf life was significantly associated with a decrease in concentration by 3,92% (3,16-4,68) for each year of storage (p <0,0001). For 509 samples, the DNA concentration was measured twice, immediately after isolation and after 4,5 years of DNA storage at -32О C. During storage, the concentration of DNA increased by an average of 2% (p=0,046).Conclusion. Long-term storage of whole blood samples at temperature from -22О C to -32О C is associated with a decrease in the DNA yield. Long-term storage of the isolated DNA at a temperature of -32О C is not associated with a decrease in its concentration.

scholarly journals Kan apolipoprotein-nivåer målt fra opptinte blodprøver etter 12-14 års nedfrysing brukes for epidemiologiske formål? En pilotanalyse av HUNT 2-data

2011 ◽  
Vol 20 (1) ◽  
Author(s):  
Ingar Holme ◽  
Bjarte Storaas ◽  
Kristian Bjerve ◽  
Arne T. Høstmark ◽  
Jostein Holmen
Keyword(s):  
Apolipoprotein B ◽  
Density Lipoprotein ◽  
English Summary ◽  
Lipoprotein Cholesterol ◽  
Font Size ◽  
Blood Samples ◽  
Long Term Storage ◽  
Apolipoprotein A ◽  
Term Storage

<span style="font-size: x-small; font-family: TimesNewRomanPSMT;"><span style="font-size: x-small; font-family: TimesNewRomanPSMT;"><p align="left">I Norge er det lagret store mengder nedfrosne blodprøver fra helseundersøkelser av ulike slag. Stabilitet av målinger av apolipoproteiner fra langtids nedfrosne blodprøver er lite dokumentert. Ny forståelse av lipoproteinenes fysiologi og betydning for utvikling av ateromatose reiser spørsmålet om andre lipoproteinkomponenter enn total (TC), LDL (LDL-C) og HDL-kolesterol (HDL-C) bør vurderes som risikomarkører. Ikke minst gjelder dette apolipoproteinene og balansen mellom apolipoprotein B (apoB) og apolipoprotein A-1 (apoA-1). Nedfrosne blodprøver kunne kanskje anvendes til å analysere sammenheng mellom nivåer på apolipoproteiner og utvikling av kardiovaskulære hendelser, hvis det ikke har skjedd vesentlige konsentrasjonsforandringer under lagring. For å undersøke dette ble det foretatt en pilotstudie av blodprøver fra 300 personer i HUNT 2. For disse hadde en ferske blodprøver, og i 2009 opptinte blodprøvemålinger av lipoproteiner samt apoB og apo A-1. Disse har bare vært lagret ved –40 ºC. Resultatene viste at TC var øket med 0,3 mmol/L fra fersk til smeltet tilstand til tross for at HDL-C var blitt redusert med 0,25 mmol/L. Korrelasjonene mellom ferske og opptinte prøver var høye (0,93-0,99). Variasjonskoeffisientene var derimot høye (5,8-13,7%) og endringene var nivåavhengige. Nivåene på apolipoproteinene var klart høyere, mens apoB/apoA-1 ratio samsvarte mer med andre studierapporter. Korrelasjonene mellom apo-og lipoproteiner var på et nivå man gjenfinner i andre studier. Resultatene må tolkes som bare indikative på at apolipoproteiner, og da spesielt ratio apoB/apoA-1, kan brukes i HUNT 2 til prognostiske studier av kardiovaskulære hendelser og død. Hoveddelen av HUNT 2 biobank har imidlertid ligget i ro i –80 ºC og lipoproteiner endres neppe ved denne temperatur. Prognostiske studier med apolipoproteiner som prediktorer burde derfor med rimelig sikkerhet kunne utføres i HUNT 2. Endelig bevis for dette kan imidlertid først oppnås når slike opptinte prøver blir analysert.</p><span style="font-size: x-small; font-family: TimesNewRomanPSMT;"><span style="font-size: x-small; font-family: TimesNewRomanPSMT;"><span style="font-size: x-small; font-family: TimesNewRomanPS-BoldMT;"><span style="font-size: x-small; font-family: TimesNewRomanPS-BoldMT;"><p align="left">Can frozen and thawed blood samples stored for 12-14 years be used to measure levels of apolipoproteins? Analysis from HUNT 2 data.            <br />English Summary: In Norway, large numbers of frozen blood samples from health surveys are stored in freezers at –70 to –80 ºC. An assumption has been that levels of lipoproteins (total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG)) have been kept at constant levels during long-term storage. The stability of long-term storage of apolipoproteins is weakly documented. The Health Survey of Nord-Trøndelag in 1995-97 (HUNT 2) provides the possibility to analyse relationships between levels of lipoproteins and incident cardiovascular disease. A project group consisting of the present authors questioned whether use of apolipoproteins as risk markers may give at least as good predictive value as the traditional lipoproteins and whether the frozen samples in HUNT 2 can be used for this purpose. To investigate this issue a pilot study based on 300 thawed blood samples from HUNT 2 was performed. For these subjects lipoprotein levels measured at screening in 1995-97 were available as well as the same levels from the thawed samples including also apolipoprotein B (apoB) and apolipoprotein A-1 (apoA-1). For special reasons these samples had only been frozen down to –40 ºC.</p></span></span></span></span></span></span>

Evaluation of a new serum separator.

1982 ◽  
Vol 28 (1) ◽  
pp. 157-159 ◽  
Author(s):  
D L Seckinger ◽  
D Antonio Vazquez ◽  
P K Rosenthal ◽  
Z H Heller
Keyword(s):  
Statistical Analysis ◽  
Chemical Analysis ◽  
T Test ◽  
Clinical Usefulness ◽  
Blood Samples ◽  
Effective Barrier ◽  
Long Term Storage ◽  
Term Storage ◽  
Paired T Test

Abstract We tested the Centri-Sep filter (DADE) for its effectiveness in separating serum from clotted blood samples used for chemical analysis. Although statistical analysis by paired t-test showed differences in results for some analytes with the use of this device as compared with use of no separator or of a serum-decanting device, we concluded from the small bias of the paired means that the new separator device did not interfere with the clinical usefulness of reported values for the analytes studied. The separator is not an effective barrier for long-term storage of serum on its clot; however, we could obtain about 10% more serum with the separator than with decanting devices.

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