Detection of FOX-1 AmpC β-lactamase Gene among Klebsiella Pneumoniae Isolated from Abattoir Samples = الكشف عن جينات فوكس ون أمب سي بيتالاكتاميز في عينات الكلبسيلة الرئوية المعزولة من عينات المجازر

2018 ◽  
Vol 54 (2) ◽  
pp. 121-128
Author(s):  
Chika Ejikeugwu ◽  
Esimone Charles ◽  
Iroha Ifeanyichukwu ◽  
Adikwu Michael
Keyword(s):  
Klebsiella Pneumoniae ◽  
Lactamase Gene

scholarly journals Enterobacteriaceae isolates carrying the New Delhi metallo-β-lactamase gene in Yemen

2014 ◽  
Vol 63 (10) ◽  
pp. 1316-1323 ◽  
Author(s):  
Alima Gharout-Sait ◽  
Samer-Ahmed Alsharapy ◽  
Lucien Brasme ◽  
Abdelaziz Touati ◽  
Rachida Kermas ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Susceptibility Testing ◽  
Enterobacter Cloacae ◽  
New Delhi ◽  
Carbapenem Resistant ◽  
Genes Encoding ◽  
Phenotypic Tests ◽  
Lactamase Gene ◽  
Sequence Types

Ten carbapenem-resistant Enterobacteriaceae (eight Klebsiella pneumoniae isolates and two Enterobacter cloacae) isolates from Yemen were investigated using in vitro antimicrobial susceptibility testing, phenotypic carbapenemase detection, multilocus sequence typing (MLST) and replicon typing. Carbapenemase, extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance determinant genes were identified using PCR and sequencing. All of the 10 carbapenem-resistant Enterobacteriaceae were resistant to β-lactams, tobramycin, ciprofloxacin and cotrimoxazole. Imipenem, doripenem and meropenem MICs ranged from 2 to >32 mg l−1 and ertapenem MICs ranged from 6 to >32 mg l−1. All of the K. pneumoniae isolates showed ESBL activity in phenotypic tests. Genes encoding bla NDM were detected in all strains. All K. pneumoniae strains produced CTX-M-15 ESBL and SHV β-lactamases. TEM-1 β-lactamase was detected in seven isolates. Nine isolates were qnr positive including QnrB1, QnrA1 and QnrS1, and six isolates produced AAC-6′-Ib-cr. MLST identified five different sequence types (STs): ST1399, ST147, ST29, ST405 and ST340. Replicon typing showed the presence of IncFII1K plasmids in four transformants. To the best of our knowledge, this is the first report of NDM-1-producing Enterobacteriaceae isolates in Yemen.

scholarly journals Isolation and Characterization of Novel Phages Targeting Pathogenic Klebsiella pneumoniae

2021 ◽  
Vol 11 ◽  
Author(s):  
Na Li ◽  
Yigang Zeng ◽  
Rong Bao ◽  
Tongyu Zhu ◽  
Demeng Tan ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
S1 Nuclease ◽  
Isolation And Characterization ◽  
Comprehensive Knowledge ◽  
Future Challenges ◽  
Lactamase Gene

Klebsiella pneumoniae is a dominant cause of community-acquired and nosocomial infections, specifically among immunocompromised individuals. The increasing occurrence of multidrug-resistant (MDR) isolates has significantly impacted the effectiveness of antimicrobial agents. As antibiotic resistance is becoming increasingly prevalent worldwide, the use of bacteriophages to treat pathogenic bacterial infections has recently gained attention. Elucidating the details of phage-bacteria interactions will provide insights into phage biology and the better development of phage therapy. In this study, a total of 22 K. pneumoniae isolates were assessed for their genetic and phenotypic relatedness by multi-locus sequence typing (MLST), endonuclease S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), and in vitro antibiotic susceptibility testing. In addition, the beta-lactamase gene (blaKPC) was characterized to determine the spread and outbreak of K. pneumoniae carbapenemase (KPC)-producing enterobacterial pathogens. Using these ST11 carbapenem-resistant K. pneumoniae isolates, three phages (NL_ZS_1, NL_ZS_2, and NL_ZS_3) from the family of Podoviridae were isolated and characterized to evaluate the application of lytic phages against the MDR K. pneumoniae isolates. In vitro inhibition assays with three phages and K. pneumoniae strain ZS15 demonstrated the strong lytic potential of the phages, however, followed by the rapid growth of phage-resistant and phage-sensitive mutants, suggesting several anti-phage mechanisms had developed in the host populations. Together, this data adds more comprehensive knowledge to known phage biology and further emphasizes their complexity and future challenges to overcome prior to using phages for controlling this important MDR bacterium.

scholarly journals Sequence of pNL194, a 79.3-Kilobase IncN Plasmid Carrying the blaVIM-1 Metallo-β-Lactamase Gene in Klebsiella pneumoniae

2010 ◽  
Vol 54 (10) ◽  
pp. 4497-4502 ◽  
Author(s):  
V. Miriagou ◽  
C. C. Papagiannitsis ◽  
S. D. Kotsakis ◽  
A. Loli ◽  
E. Tzelepi ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Mobile Elements ◽  
Mosaic Structure ◽  
Lactamase Gene ◽  
Characteristic Segment

ABSTRACT The nucleotide sequence of pNL194, a VIM-1-encoding plasmid, is described in this study. pNL194 (79,307 bp) comprised an IncN-characteristic segment (38,940 bp) and a mosaic structure (40,367 bp) including bla VIM-1, aacA7, aadA1, aadA2, dfrA1, dfrA12, aphA1, strA, strB, and sul1. Tn1000 or Tn5501 insertion within fipA probably facilitated recruitment of additional mobile elements carrying resistance genes.

scholarly journals Characterization of the Inhibitor-Resistant SHV β-Lactamase SHV-107 in a Clinical Klebsiella pneumoniae Strain Coproducing GES-7 Enzyme

2011 ◽  
Vol 56 (2) ◽  
pp. 1042-1046 ◽  
Author(s):  
Vera Manageiro ◽  
Eugénia Ferreira ◽  
Antony Cougnoux ◽  
Luís Albuquerque ◽  
Manuela Caniça ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Catalytic Efficiency ◽  
Dynamics Simulation ◽  
Inhibitory Effects ◽  
Content Type ◽  
High Affinity ◽  
Amoxicillin Clavulanate ◽  
Lactamase Gene

ABSTRACTThe clinicalKlebsiella pneumoniaeINSRA6884 strain exhibited nonsusceptibility to all penicillins tested (MICs of 64 to >2,048 μg/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2,048 to 512 μg/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genesblaGES-7and a new β-lactamase gene,blaSHV-107, which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producingEscherichia colistrain exhibited only a β-lactam resistance phenotype with respect to amoxicillin, ticarcillin, and amoxicillin-clavulanate combination. The kinetic parameters of the purified SHV-107 enzyme revealed a high affinity for penicillins. However, catalytic efficiency for these antibiotics was lower for SHV-107 than for SHV-1. No hydrolysis was detected against oxyimino-β-lactams. The 50% inhibitory concentration (IC50) for clavulanic acid was 9-fold higher for SHV-107 than for SHV-1, but the inhibitory effects of tazobactam were unchanged. Molecular dynamics simulation suggested that the Thr235Ala substitution affects the accommodation of clavulanate in the binding site and therefore its inhibitory activity.

scholarly journals Novel Genetic Environment of the Carbapenem-Hydrolyzing β-Lactamase KPC-2 among Enterobacteriaceae in China

2009 ◽  
Vol 53 (10) ◽  
pp. 4333-4338 ◽  
Author(s):  
Ping Shen ◽  
Zeqing Wei ◽  
Yan Jiang ◽  
Xiaoxing Du ◽  
Shujuan Ji ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Gene Order ◽  
Gel Electrophoresis ◽  
Eastern China ◽  
Carbapenem Resistance ◽  
Pulsed Field Gel Electrophoresis ◽  
Genetic Environment ◽  
The Family ◽  
Lactamase Gene

ABSTRACT Thirty-nine bla KPC-producing isolates of the family Enterobacteriaceae with carbapenem resistance or reduced carbapenem susceptibility were obtained from inpatients from eight hospitals in six cities of three provinces in eastern China. The pulsed-field gel electrophoresis analysis of all 36 Klebsiella pneumoniae isolates revealed six major patterns. The resistant plasmids of most isolates were successfully transferred by conjugation and evaluated experimentally to be 40 to 180 kb in size. A 20.2-kb bla KPC-surrounding nucleotide sequence from plasmid pKP048 has been obtained and contains an integration structure of a Tn3-based transposon and partial Tn4401 segment, with the gene order Tn3-transposase, Tn3-resolvase, ISKpn8, the bla KPC-2 gene, and the ISKpn6-like element. The chimera of several transposon-associated elements indicated a novel genetic environment of the K. pneumoniae carbapenemase β-lactamase gene in isolates from China.

scholarly journals Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

2012 ◽  
Vol 79 (3) ◽  
pp. 1052-1054 ◽  
Author(s):  
Dixie F. Mollenkopf ◽  
Jennifer M. Mirecki ◽  
Joshua B. Daniels ◽  
Julie A. Funk ◽  
Steven C. Henry ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Use ◽  
Fecal Samples ◽  
Content Type ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACTWe report the recovery ofEscherichia coliorKlebsiella pneumoniaecontaining the extended-spectrum β-lactamase geneblaCTX-Mfrom 24 of 1,495 (1.6%) swine fecal samples in 8 of 50 (16%) finishing barns located in 5 U.S. states. We did not detect an association between antimicrobial use and recovery ofblaCTX-M.

scholarly journals Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

2012 ◽  
Vol 6 (05) ◽  
pp. 457-461 ◽  
Author(s):  
Rima I El-Herte ◽  
George F Araj ◽  
Ghassan M Matar ◽  
Maysa Baroud ◽  
Zeina A Kanafani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
New Delhi ◽  
Beta Lactamase ◽  
The Novel ◽  
First Report ◽  
Carbapenem Resistant ◽  
Lactamase Gene ◽  
Carbapenem Resistant Enterobacteriaceae

Carbapenem resistance has been encountered globally with poor outcome of infected patients. NDM-1 (New Delhi metallo-beta-lactamase) gene containing organisms have emerged and are now spreading in all continents. This is the first report of Iraqi patients referred to Lebanon from whom carbapenem resistant Enterobacteriaceae were recovered. The genes involved in carbapenem resistance were bla-OXA-48   and the novel NDM-1. This report highlights the alarming introduction of such resistance among Enterobacteriaecae to this country.

scholarly journals Distribution of β-lactamase genes of Klebsiella pneumoniae isolates in Zhejiang province, China, and regulation of gene expression

2017 ◽  
Vol 69 (3) ◽  
pp. 399-407
Author(s):  
Jin-Fang Zhao ◽  
Qiang Wang ◽  
Yu-Mei Ge ◽  
Pan-Li Tan ◽  
Yi-Min Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Klebsiella Pneumoniae ◽  
Histidine Kinase ◽  
Kinase Inhibitor ◽  
Regulation Of Gene Expression ◽  
Confirmatory Test ◽  
Signaling Systems ◽  
Qrt Pcr ◽  
Lactamase Gene ◽  
M 14

Klebsiella pneumoniae is a common causative agent of nosocomial infections with a high level of resistance toward ?-lactam antibiotics. Our previous study showed that TEM-1 and SHV-11 are the predominant ?-lactamase-encoding genes of K. pneumoniae isolates in the Zhejiangarea, China. In this study, more clinical K. pneumoniae isolates were collected for detecting their ?-lactamase-encoding gene profiles by PCR and sequencing. qRT-PCR was then performed to determine the role of cefotaxime or penicillin in low concentrations to induce the ?-lactamase gene expression of K. pneumoniae isolates. Moreover, the K. pneumoniae isolates were pretreated with closantel (CLO), a histidine kinase inhibitor, before antibiotic treatment, and qRT-PCR and the ?-lactamase phenotype confirmatory test were then applied to determine the effect of CLO on the expression of the ?-lactamase genes. The results showed that, except for KPC-2, the 1/4 MIC cefotaxime or penicillin induced significant mRNA elevation of the TEM-1, CTX-M-14, SHV-11 and OXA-1?-lactamase genes, but this induction could be inhibited by CLO. After pretreatment withCLO,78.4~81.4%of the ?-lactam-resistant isolates became sensitive and the positive rate of the ?-lactamase production phenotype in the isolates was decreased from 100% to 27.1%. The data indicate thatTEM-1 (70.7%), SHV-11 (64.2%) and CTX-M-14 (40.5%) are the predominant ?-lactamase genes of the K. pneumoniae isolates in Zhejiang and sublethal dosage of ?-lactam antibiotics can induce the ?-lactamase gene expression of K. pneumoniae through histidine kinase-mediated two-component signaling systems.

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