Genetic Diversity, Structure and DNA Fingerprint for Developing Molecular IDs of Yemeni Coffee (Coffea Arabica L.) Germplasm Assessed by SSR Markers = الاختلافات والعلاقات الوراثية وتحديد البصمة الوراثية لإنشاء تعريف جزيئي للأصول الوراثية للبن اليمني العربي بواسطة معلمات التتابعات المكررة البسيطة (الميكروستالليت)

2017 ◽  
Vol 21 (4) ◽  
pp. 713-736
Author(s):  
M. A. A. Hussein ◽  
A. A. A. Al-Azab ◽  
S. S. Habib
Author(s):  
C. Montagnon ◽  
A. Mahyoub ◽  
W. Solano ◽  
F. Sheibani

AbstractWhilst it is established that almost all cultivated coffee (Coffea arabica L.) varieties originated in Yemen after some coffee seeds were introduced into Yemen from neighboring Ethiopia, the actual coffee genetic diversity in Yemen and its significance to the coffee world had never been explored. We observed five genetic clusters. The first cluster, which we named the Ethiopian-Only (EO) cluster, was made up exclusively of the Ethiopian accessions. This cluster was clearly separated from the Yemen and cultivated varieties clusters, hence confirming the genetic distance between wild Ethiopian accessions and coffee cultivated varieties around the world. The second cluster, which we named the SL-17 cluster, was a small cluster of cultivated worldwide varieties and included no Yemen samples. Two other clusters were made up of worldwide varieties and Yemen samples. We named these the Yemen Typica-Bourbon cluster and the Yemen SL-34 cluster. Finally, we observed one cluster that was unique to Yemen and was not related to any known cultivated varieties and not even to any known Ethiopian accession: we name this cluster the New-Yemen cluster. We discuss the consequences of these findings and their potential to pave the way for further comprehensive genetic improvement projects for the identification of major resilience/adaptation and cup quality genes that have been shaped through the domestication process of C. arabica.


2016 ◽  
Vol 7 (2) ◽  
pp. 76
Author(s):  
Dwinita Wikan Utami ◽  
Sutoro Sutoro ◽  
Nurul Hidayatun ◽  
Andari Risliawati ◽  
Ida Hanarida

<p>Genetic Diversity of 96 Accession of Rice Germplasm<br />Using 30 SSR Markers Linked to Heading Date Genes (HD<br />Genes). Dwinita W. Utami, Sutoro, Nurul Hidayatun,<br />Andari Risliawati, and Ida Hanarida. Rice with early<br />maturity is one of an important genetic resources in rice<br />germplasm collection. Characterization and identification of<br />genetic diversity is an important issue for plant variety protection.<br />Molecular identification by microsatellite markers<br />using Genetic Analyzer enables resolve of this issue. The<br />objective of this research is to identify the genetic diversity of<br />96 rice accessions based on their specific DNA fingerprint<br />using microsatellite markers. A total of 96 accessions consisting<br />of a diverse variety of maturity classification were<br />genotyped with 30 SSR markers linked to HD genes which<br />spread out in 12 chromosomes of rice geneome. The total<br />297 alleles were detected indicated the level of marker<br />informativeness. RM5607 generated 7 allele with the size<br />range from 103 to 197 and the highest PIC at 0.90. RM3571<br />(linked to HD12 gene) has a significant value associated with<br />varieties which have early maturity trait. Clustering analysis<br />showed the cluster based on Sub Species genome background<br />and on early maturity trait.</p>


2020 ◽  
Vol 7 (3) ◽  
pp. 223-229
Author(s):  
Afework Legesse

Coffee is an important source of annual income and employment contributing significantly to the economies of many developing countries. Ethiopia is the center of origin and diversity of Coffea arabica L., there is immense genetic variability that offers great potential for improvement of the crop. The objective of this paper is to assess the status of Coffee genetic diversity, identify major factors that cause coffee genetic erosion and status of coffee genetics resources management in Ethiopia.  The presences of high genetic diversity in wild Coffea arabica in Ethiopia were reported by different authors. However, the genetic diversity of coffea arabica L. are being lost rapidly due to several factors such as human population pressures leading to conversion of land to agriculture, deforestation and land degradation; low coffee prices leading to abandoning of coffee trees in forests and gardens and shifting cultivation to other more remunerative crops; and climate change. Additionally, narrow genetic basis of commercially used Arabica coffee cultivars and increased incidence of pests and diseases associated with climate change is leading to significant crop losses, threatening livelihoods in many coffee growing countries. Therefore, Conserving the wild Arabica coffee gene pool and its evolutionary potential present in Ethiopia is critically important for maintaining coffee yield, disease resistance, drought tolerant, quality and other important traits in future breeding program


2020 ◽  
Vol 77 (3) ◽  
Author(s):  
Alexandra Dias ◽  
Guia Giovannelli ◽  
Bruno Fady ◽  
Ilaria Spanu ◽  
Giovanni G Vendramin ◽  
...  

2011 ◽  
Vol 62 (2) ◽  
pp. 177 ◽  
Author(s):  
Tadesse Sefera ◽  
Bekele Abebie ◽  
Pooran M. Gaur ◽  
Kebebew Assefa ◽  
Rajeev K. Varshney

The genomic DNA profiles of 48 chickpea cultivars released in nine countries and of historical significance to the chickpea breeding programs at ICRISAT and in Ethiopia were evaluated using 48 simple sequence repeat (SSR) markers. Across the cultivars, a total of 504 alleles representing the 48 SSR loci were detected with frequencies ranging from three to 22 (mean 10.5) alleles per locus. The polymorphism information content (PIC) for the SSR markers varied from 0.37 to 0.91 (mean 0.77). A subset of only three highly informative SSR markers (TA176, TA2, TA180) enabled complete discrimination among all 48 chickpea cultivars tested. Hierarchical neighbour-joining UPGMA cluster analysis based on simple matching dissimilarity matrix resolved the 48 cultivars into two major clusters representing desi and kabuli types. These cluster groupings of the cultivars were consistent with the pedigree information available for the cultivars as to the phenotypic classes of chickpea types. Analysis of the temporal patterns of the SSR diversity by classifying 48 chickpea cultivars into four periods of release revealed increasing tendencies in the overall genetic diversity from 0.42 for the earliest varieties developed in the 1970s to 0.62 for those released in the 1980s, and reached a maximum and equivalent level of 0.72 for the varieties developed in the 1990s and 2000s. Overall, the study ascertained that SSRs provide powerful marker tools in revealing genetic diversity and relationships in chickpeas, thereby proving useful for selection of parents in breeding programs and also for DNA fingerprint identification of cultivars.


2020 ◽  
Author(s):  
Juliano Lino Ferreira ◽  
Eveline Teixeira Caixeta ◽  
Fernanda Fatima Caniato ◽  
Tesfahun Setotaw ◽  
Gustavo César Sant’Ana ◽  
...  

Coffea arabica L. is a native coffee species probably originated in Abyssinia, now Ethiopia. The genetic diversity of C. arabica has economic implications directly related to profits by breeding for developing new varieties to a global market. The economic value of C. arabica genetic resources are estimated at US$ 420 million, considered a 10% discount rate. Understanding the extent of traits variability and genetic diversity is essential to guide crosses between genotypes, targeting the development of new varieties with high economic value. This chapter will present the C. arabica economic importance, primarily to Brazil, the most significant world producer; we will outline the origin and dispersion of arabica coffee and briefly show the leading germplasm banks. We will also point out contribution of genetic diversity studies based on morphological, agronomic traits, and molecular markers supporting the development of new varieties. Finally, we present an outline for the future.


2009 ◽  
Vol 9 (4) ◽  
pp. 361-371 ◽  
Author(s):  
R.F. Missio ◽  
E.T. Caixeta ◽  
E.M. Zambolim ◽  
L. Zambolim ◽  
N.S. Sakiyama

2007 ◽  
Vol 54 (6) ◽  
pp. 1367-1379 ◽  
Author(s):  
Milene Silvestrini ◽  
Michele G. Junqueira ◽  
Andréa C. Favarin ◽  
Oliveiro Guerreiro-Filho ◽  
Mirian P. Maluf ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document