Breeding for Rust Resistance and Some Economic Characters in Snap Bean = التربية لمقاومة الصدأ وبعض الصفات الاقتصادية في الفاصوليا الخضراء

2017 ◽  
Vol 21 (2) ◽  
pp. 339-361
Author(s):  
A. A. Hamed ◽  
Naglaa A. S. Muhanna
Keyword(s):  
2010 ◽  
Vol 135 (6) ◽  
pp. 521-532 ◽  
Author(s):  
Charles J. Wasonga ◽  
Marcial A. Pastor-Corrales ◽  
Timothy G. Porch ◽  
Phillip D. Griffiths

Common bean rust disease (caused by Uromyces appendiculatus) and high temperatures (heat stress) limit snap bean (Phaseolus vulgaris) production in many tropical and temperate regions. We have developed snap bean lines combining broad-spectrum rust resistance with heat tolerance for tropical agroecosystems. Eight breeding populations were developed by hybridizing BelJersey-RR-15 and BelFla-RR-1 (each possessing the Ur-4 and Ur-11 rust resistance genes) and the heat-tolerant snap bean breeding lines HT601, HT603, HT608, and HT611. F2–F4 generations of the populations were evaluated under greenhouse conditions and selected for heat tolerance while simultaneously selecting for the rust resistance genes Ur-4 and Ur-11. Three heat-tolerant F5 lines, which were homozygous for Ur-4 and Ur-11 genes, were selected together with a rust-resistant but heat-sensitive control. These and 12 cultivars adapted to different geographical regions were evaluated for their reaction to rust and yield at six contrasting field sites in eastern Africa and their response to high temperature verified in Puerto Rico. Rust incidence and severity was high at three of the trial sites in eastern Africa. Two of the 12 cultivars were resistant to rust at most of these sites, and three of the four breeding lines were resistant at all sites. The Ur-11 gene effectively conferred rust resistance at all sites. Yield in Puerto Rico was strongly correlated (R2 = 0.71, P < 0.001) with that of the hottest site in eastern Africa, highlighting the similarity in genotypic response to high temperatures at the two distinct sites. The newly developed rust-resistant and heat-tolerant breeding lines showed stable yield at the eastern Africa sites with contrasting mean temperatures compared with the cultivars presently grown in the region. Two of these lines, HT1 and HT2, were confirmed to be homozygous for Ur-4 and Ur-11 and with high heat tolerance under both greenhouse and field environments. This research validates the effectiveness of targeted rust resistance gene combinations for tropical environments and the effective selection of high temperature tolerance traits correlating across multiple environments. The breeding lines HT1 and HT2 developed in this research could be used to improve snap beans for the tropics and other environments with similar constraints.


2012 ◽  
Vol 16 (1) ◽  
pp. 99-113
Author(s):  
A. A. Hamed ◽  
Naglaa A. S. Muhanna ◽  
A. M. Abd Rabou

2021 ◽  
Vol 33 (1) ◽  
pp. 57
Author(s):  
H. M. P. S. Kumari ◽  
C. K. Weebadde ◽  
P. C. G. Bandaranayake ◽  
M. A. Pastor Corrales ◽  
R. G. A. S. Rajapakshe

Author(s):  
D.W.R. White

Cell culture and genetic engineering techniques can be used to develop improved pasture plants. To utilise these methods we have developed procedures for regenerating plants from tissue cultures of perennial ryegrass and white clover. In both, the plant genotype influences regeneration capacity. There was significant genetic variation among regenerated perennial ryegrass plants in a wide range of characteristics. Most of the regenerants were resIstant to crown rust and this trait was highly heritable. This rust resistance is being used to breed a new ryegrass cultivar. A system for introducing cloned genes into white clover is described. This capability is bemg used to incorporate genes with the potential to improve nutritional quality and pest resistance. Other possibilities for engineering genetic improvements in white clover, genes conferring herbicide tolerance and resistance to white clover mosaic virus, are briefly outlined. Keywords: Lolium perenne, Trifolium repens, cell culture, somaclonal variation, crown rust resistance, transformation, cloned genes, nutritional quality, proteinase inhibitors, Bt toxins, pest resistance, WCMV viral cross-protection, herbicide tolerance, Agrobacterium, Bacillus thuringenisis.


Author(s):  
Pratima Sharma ◽  
Madhu Patial ◽  
Dharam Pal ◽  
S. C. Bhardwaj ◽  
Subodh . Kumar ◽  
...  

The present study was conducted to transfer multiple rust resistance in a popular but rust susceptible wheat cultivar HS295. Selected derivatives WBM3632 and WBM3635 have been developed from a cross, HS295*2/FLW20//HS295*2/ FLW13 using bulk-pedigree method of breeding. Advance line WBM3697 selected from a breeding line WBM3532 was named as HS661. This line was evaluated for seedling resistance to a wide array of rust pathotypes and found to possess resistance to all the three rusts. HS661 was also tested under field conditions and showed adult plant resistance to leaf rust (AC1=0.6), stem rust (ACI=2.7) and strpe rust (AC1=3.8). Among 34 F3 lines, 28 were tested positive for SSR marker Xwmc221 indicating the presence of Lr19/Sr25. Out of 14 selected F4 lines from F3, nine were homozygous positive for Lr19/Sr25. The advanced breeding lines viz., WBM3632 (WBM3697) and WBM3635 were also positive for Lr19/Sr25 with SCAR marker SCS265512. SSR marker Xgwm1 producing 215 bp band in Avst-15, FLW13 and HS661 confirmed the presence of Yr15 . Agronomically, HS661 was comparable with recipient variety HS295 and superior to a standard check HS490 under late sown restricted irrigation production conditions of NHZ. HS661 may serve as a potential donor for creating new usable variability against all the three rusts.


2010 ◽  
Vol 36 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Hong ZHANG ◽  
Zhi-Long REN ◽  
Yin-Gang HU ◽  
Chang-You WANG ◽  
Wan-Quan JI

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