Molecular and Serological Assessment of Chronic Parvovirus B19 among Chronic Hemolytic Anemia Children

2017 ◽  
Vol 26 (1) ◽  
pp. 17-24
Author(s):  
Amal F. Makled ◽  
Ahmed A. Salama ◽  
Mahmoud A. Elhawy
Keyword(s):  
Hemolytic Anemia ◽  
Parvovirus B19 ◽  
Chronic Hemolytic Anemia

Detection of Human Parvovirus B19 Infection in Children with Chronic Haemolytic Anaemia in Benha University Hospitals

2021 ◽  
Vol 30 (2) ◽  
pp. 51-58
Author(s):  
Amal M. Matta ◽  
Elsayed M. Abd-Elghany ◽  
Abeer A. Aboelazm ◽  
Osama Abo. Zaki, ◽  
Doaa Abd. Shaker
Keyword(s):  
Hemolytic Anemia ◽  
Parvovirus B19 ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Igg Antibodies ◽  
University Hospitals ◽  
History Of ◽  
Chronic Hemolytic Anemia ◽  
Apparently Healthy

Background: Due to the tropism of human parvovirus B19 to erythroid progenitor cells, infection in patients with an underlying hemolytic disorder such as thalassemia, hereditary spherocytosis, sickle cell disease and Glucose-6-phosphate dehydrogenase deficiency leads to suppression of erythrocyte formation, referred to as transient aplasia crisis (TAC), which may be life-threatening. Objectives: Detection of parvovirus B19 DNA and its IgG antibodies in the serum of children with chronic hemolytic anemia and in apparently healthy children in Benha University Hospitals. Methodology: The study was conducted on 80 children. Forty of them with chronic hemolytic anemia, they were subdivided into 2 groups, Group (1a) included 20 patients without history of aplastic crisis, Group (Ib) included 20 patients with a history of aplastic crisis and 40 age and sex-matched apparently healthy children representing control (Group II). All patients were subjected to full history taking, clinical examination and laboratory investigations. Parvovirus B19 IgG was measured using anti-parvovirus B19 ELISA kits (SUNRED), and parvovirus B19 DNA was detected by using nestedpolymerase chain reaction. Results: The seroprevalence of parvovirus B19 IgG was significantly higher (P value =0.016) in Group Ia (50%) (10 out of 20) and Group Ib (45%) (9 out of 20) than the control group (Group II) (17.5%) (7 out of 40). There was a significant positive correlation between anti-parvovirus B19 IgG and age of all patients, frequency of blood transfusion. The prevalence of parvovirus B19 DNA was 10% (2 out of 20) in group Ia and 30% (6 out of 20) in group Ib and no viral DNA was detected in the controls (P value=0.001). Although 42.3% (11 out of 26) of children with β thalassemia major had a detectable level of antiparvovirus B19 virus IgG antibodies, only (23.1%) (6 out of 26) of them had B19 DNA. Anti-parvovirus B19 IgG antibodies were detected in 4 children out of 5 children of sickle cell anemia (80%) but the the prevalence of Parvovirus B19 DNA was 20% among them. Conclusion: Measures to keep away from iatrogenic and nosocomial infection transmission should be implemented including screening of donated blood for parvovirus B19 especially blood given to patients with blood disorders. Recommendation: Data from this study support the need for introduction of an approved vaccine that mainly protects children with chronic hemolytic anemia against that infection.

scholarly journals Study of chronic hemolytic anaemia patients in Rio de Janeiro: prevalence of anti-human parvovirus B19 IgG antibodies and the developement aplastic crises

2002 ◽  
Vol 44 (4) ◽  
pp. 187-190 ◽  
Author(s):  
Anadayr L.M. SANT'ANNA ◽  
Rita de Cássia N. Cubel GARCIA ◽  
Mônica MARZOCHE ◽  
Heloisa Helena A. Gallo da ROCHA ◽  
Maria Tereza M. PAULA ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Hemolytic Anemia ◽  
Sickle Cell ◽  
Parvovirus B19 ◽  
Human Parvovirus B19 ◽  
Igg Antibodies ◽  
Cell Disease ◽  
Antibody Prevalence ◽  
Igg Antibody ◽  
Chronic Hemolytic Anemia

The prevalence of anti-human parvovirus B19 IgG antibodies was determined in sera from 165 chronic hemolytic anemia patients, receiving medical care at Instituto Estadual de Hematologia (IEHE), Rio de Janeiro, during the year of 1994. This sample represents around 10% of the chronic hemolytic anemia patients attending at IEHE. Most of these patients (140) have sickle cell disease. Anti-B19 IgG antibodies were detected in 32.1% of patients. No statistically significant difference (p > 0.05) was seen between IgG antibody prevalence in male (27.8%) and female (35.5%) patients. Anti-B19 IgG antibodies were more frequent in older (37.6%) than younger (28.2%) than 20 years old patients, although this difference had no statistical significance (p > 0.05). Anti-B19 IgG antibody prevalence showed that 67.9% of patients enrolled in the study were susceptible to B19 acute infection. With the aim to detect acute B19 infection, patients follow up continued until February 1996. During this period four patients presented transient aplastic crisis due to human parvovirus B19 as confirmed by the detection of specific IgM antibodies. All four patients were younger than 20 years old, and 3 were younger than 10 years old. Three of them were sickle cell disease patients. Three of the four acute B19 infection occurred during 1994 springtime.

PARVOVIRUS B19 INFECTION REMINISCENT OF MYELODYSPLASTIC SYNDROME IN THREE CHILDREN WITH CHRONIC HEMOLYTIC ANEMIA

2000 ◽  
Vol 17 (6) ◽  
pp. 475-482 ◽  
Author(s):  
Neşe Yaralı ◽  
Feride Duru ◽  
Tansu Sipahi ◽  
Abdurrahman Kara ◽  
Tahsin Teziç
Keyword(s):  
Myelodysplastic Syndrome ◽  
Hemolytic Anemia ◽  
Parvovirus B19 ◽  
Parvovirus B19 Infection ◽  
Chronic Hemolytic Anemia

Pyrimidine 5′ Nucleotidase Deficiency: Clinical and Molecular Characterization of Two New Italian Patients.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3711-3711
Author(s):  
Elisa Fermo ◽  
Anna Marcello ◽  
Paola Bianchi ◽  
Simona Viglio ◽  
Laurent R. Chiarelli ◽  
...  
Keyword(s):  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Parvovirus B19 ◽  
Peripheral Blood Smear ◽  
Nucleotide Metabolism ◽  
Unconjugated Bilirubin ◽  
Molecular Characteristics ◽  
Italian Origin ◽  
Chronic Hemolytic Anemia

Abstract Hereditary pyrimidine 5′ nucleotidase deficiency (P5′N) is the most frequent abnormality of the red cell nucleotide metabolism causing hereditary non-spherocytic hemolytic anemia. The disorder is characterized by mild-to-moderate hemolytic anemia associated with reticulocytosis and hyperbilirubinemia and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. P5′N-1 gene is localized on 7p15-p14; eighteen mutations have been so far identified in 27 unrelated families, 6 of them of Italian origin. The aim of this study is to describe the hematological, biochemical and molecular characteristics of two new Italian patients affected by P5′N deficiency. Case1: The propositus was a 37 yrs old woman of Northern Italian origin affected by chronic hemolytic anemia with Hb levels ranging from 8.2 to 10.5 g/dL. At the time of the study Hb was 8.4 g/dL, reticulocytes 300x109/L, unconjugated bilirubin 3.2 mg/dL. Peripheral blood smear examination showed basophilic stippling and purines/pyrimidines ratio (OD260/280) was decreased (1, ref. values 1.4 – 2.98). P5′N activity, measured by capillary electrophoresis, was undetectable. Molecular analysis of P5′N-1 gene showed the presence of a new homozygous deletion of two bp (ag) at the splice junction between intron 7 and exon 8, which probably results in a splicing alteration and in the absence of a functional protein. Case2: The propositus, a 37 yrs old woman of Northern Italian origin carrying the hemoglobin variant HbD Punjab, had an history of chronic hemolytic anemia since childhood; at the age of 14 yrs splenectomy and colecystectomy were performed. The patient needed blood transfusions because of exacerbation of anemia (Hb 4.7g/dL) during parvovirus B19 infection. Iron status parameters were increased requiring desferrioxamine treatment. At the time of the study Hb was 9.3 g/dL, reticulocytes 752x109/L, unconjugated bilirubin 13.3 mg/dL. Serum ferritin was 1980 mg/mL and transferrin saturation 115%. The propositus was found to be homozygous for Gilbert’s syndrome and heterozygous for mutation H63D of HFE gene. Basophilic stippling (6%) was observed in peripheral blood smear. Pur/pyr ratio was 0.8 and residual P5′N activity was 40% of normal. Complete sequencing of P5′N-1 gene showed the presence of the frameshift mutation ins GG710-711, already described in Italian and Turkish patients, and the new in-frame aminoacidic deletion of Gln 143.

Henoch-Schönlein Purpura and Human Parvovirus Infection

PEDIATRICS ◽  
1986 ◽  
Vol 78 (1) ◽  
pp. 183-184
Author(s):  
J. J. LEFRÈRE ◽  
A. M. COUROUCÉ ◽  
J. P. SOULIER ◽  
M. P. CORDIER ◽  
M. C. GUESNE GIRAULT ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Hemolytic Anemia ◽  
Joint Pain ◽  
Past History ◽  
Henoch Schonlein Purpura ◽  
Erythema Infectiosum ◽  
Aplastic Crisis ◽  
Fifth Disease ◽  
Schonlein Purpura ◽  
Chronic Hemolytic Anemia

To the Editor.— Human parvovirus is already known to be responsible for aplastic crisis in chronic hemolytic anemia,1 for erythema infectiosum or fifth disease,2 and for arthropathies,3,4 and it has recently been isolated from the serum of patients with vascular purpura.5 We report the case of Henoch-Schonlein purpura associated with human parvovirus infection. H. T., a 6-year-old girl, without any significant past history, was admitted on March 8, 1985, for joint pain and swelling (wrists, knees, ankles) associated with intense abdominal pain.

scholarly journals Clinical and genetic diagnosis of thirteen Japanese patients with hereditary spherocytosis

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Keiko Shimojima Yamamoto ◽  
Taiju Utshigisawa ◽  
Hiromi Ogura ◽  
Takako Aoki ◽  
Takahiro Kawakami ◽  
...  
Keyword(s):  
Hemolytic Anemia ◽  
Hereditary Spherocytosis ◽  
Genetic Diagnosis ◽  
Genomic Analysis ◽  
Japanese Patients ◽  
Asian Countries ◽  
Target Capture ◽  
Detailed Family History ◽  
Target Capture Sequencing ◽  
Chronic Hemolytic Anemia

AbstractHereditary spherocytosis is the most frequent cause of hereditary hemolytic anemia and is classified into five subtypes (SPH1-5) according to OMIM. Because the clinical and laboratory features of patients with SPH1-5 are variable, it is difficult to classify these patients into the five subtypes based only on these features. We performed target capture sequencing in 51 patients with hemolytic anemia associated with/without morphological abnormalities in red blood cells. Thirteen variants were identified in five hereditary spherocytosis-related genes (six in ANK1 [SPH1]; four in SPTB [SPH2]; and one in each of SPTA1 [SPH3], SLC4A1 [SPH4], and EPB42 [SPH5]). Among these variants, seven were novel. The distribution pattern of the variants was different from that reported previously in Japan but similar to those reported in other Asian countries. Comprehensive genomic analysis would be useful and recommended, especially for patients without a detailed family history and those receiving frequent blood transfusions due to chronic hemolytic anemia.

The role of soluble transferrin receptor in iron overload in children with chronic hemolytic anemia

2013 ◽  
Vol 26 (2) ◽  
pp. 132
Author(s):  
RedaI Rakha ◽  
SamarM.K. El-Din Fathallah ◽  
FathiaM El-Nemr ◽  
FaridaH El-Rashidi ◽  
SehamM Ragab
Keyword(s):  
Iron Overload ◽  
Hemolytic Anemia ◽  
Transferrin Receptor ◽  
Soluble Transferrin Receptor ◽  
Chronic Hemolytic Anemia

Thrombin antithrombin complex assessment in patients with chronic hemolytic anemia as a marker for the activity of coagulation

2018 ◽  
Vol 43 (1) ◽  
pp. 10
Author(s):  
YasminN El-Sakhawy ◽  
IbrahimY Abdel-Messih ◽  
NevineG Andrawes ◽  
NesmaA Safwat ◽  
YasminH Ibrahim
Keyword(s):  
Hemolytic Anemia ◽  
Chronic Hemolytic Anemia
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