The Myrtus communis L. leaves samples were collected from five locations of its native grownareas in Lattakia, Syria, during their blooming seasons (June, 2009). Essential oil (EO) extraction wascarried out by hydro-distillation in a Clevenger apparatus. The EO was analysed by both gas chromatography-Flame Ionization Detector (GC-FID) and gas chromatography/mass (GC/MS) techniques. The EO yieldof the dry samples was found to be around 1.88%. The main identified components of EO were: µ-pinene30.40%, 1,8-cineole 17.66%, limonene 8.96%, myrtenol 5.78%, and b-caryophyllene 5.00%. The bulk EOand the separated components were tested for their antibacterial activities against Escherichia coli O157,Salmonella typhimurium, Klebsiella pneumoniae, Yersinia enterocolitica O9, Brucella melitensis, Proteusspp., and Pseudomonas aeruginosa by using broth micro-dilution method. It was found that citronellal andnerol were the most effective components against all pathogens.