Hepatitis C Virus (HCV) Core Antigen as an Alternative to PCR for HCV, RNA Quantification to Early Diagnosis of HCV Infection among Blood Donors = إمكانية استخدام مستضد لب فيروس سي كبديل لتفاعل البلمرة التسلسلي في التشخيص المبكر لفيروس سي لدى المتبرعين بالدم

2016 ◽  
Vol 45 (4) ◽  
pp. 975-984
Author(s):  
Mohamed Aleraky Saleh ◽  
Mahmoud Ali Saudi ◽  
Omima Sayed Mohamed
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Early Diagnosis ◽  
Blood Donors ◽  
Hcv Infection ◽  
Core Antigen ◽  
Hcv Rna ◽  
Rna Quantification ◽  
Hcv Core Antigen

scholarly journals New Automated Hepatitis C Virus (HCV) Core Antigen Assay as an Alternative to Real-Time PCR for HCV RNA Quantification

2010 ◽  
Vol 48 (6) ◽  
pp. 2253-2256 ◽  
Author(s):  
Y. Park ◽  
J.-H. Lee ◽  
B. S. Kim ◽  
D. Y. Kim ◽  
K.-H. Han ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Real Time Pcr ◽  
Core Antigen ◽  
Hcv Rna ◽  
Rna Quantification ◽  
Hcv Core Antigen ◽  
Antigen Assay

Serologic, Virologic, and Histologic Characteristics of Chronic Phase Hepatitis C Virus Disease in Children Infected by Transfusion

PEDIATRICS ◽  
1994 ◽  
Vol 94 (6) ◽  
pp. 919-922
Author(s):  
Suguru Matsuoka ◽  
Katsuyoshi Tatara ◽  
Yasunobu Hayabuchi ◽  
Yoshiyuki Taguchi ◽  
Kazuhiro Mori ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Acute Infection ◽  
Chronic Phase ◽  
Hcv Infection ◽  
Core Antigen ◽  
Hcv Rna ◽  
Histologic Evidence ◽  
Group A ◽  
Group B

Objective. We studied the time course of hepatic dysfunction, seropositivity to hepatitis C virus (HCV) antibodies, viremia, and histologic evidence of hepatic injury to evaluate the course of HCV infection in children infected by blood transfusion. Patients and methods. Twenty-nine patients (ages 4 to 18 years) who underwent open-heart surgeries for congenital heart disease were grouped into three categories based on alterations in serum alanine aminotransferase (ALT) levels: Group A, acute infection; Group B, subacute infection; and Group C, chronic infection. Results. In Group C, all 13 patients had detectable HCV RNA in serum. In contrast, all patients in Group A had no detectable HCV RNA. In Group B, one of nine patients had detectable HCV RNA and two of four patients examined had persistent chronic hepatitis by histologic criteria. Antibodies directed against C100-3 antigen or core-antigen were more useful than second-generation HCV antibody assays in determining the relationship between viremia and immunologic response. Infection with HCV genotype II and the presence of higher HCV RNA copy numbers were associated with histologic evidence of hepatic damage. Conclusion. An abnormal ALT value is frequently associated with viremia, and biochemically resolved acute infection reflects clearance of HCV. However, a normal ALT does not always reflect an absence of hepatocyte damage and HCV replication in patients with subacute disease. The measures outlined in this study are useful indicators of disease activity during the chronic phase of post-transfusion HCV infection.

scholarly journals Simultaneous Detection of Hepatitis C Virus (HCV) Core Antigen and Anti-HCV Antibodies Improves the Early Detection of HCV Infection

2005 ◽  
Vol 43 (8) ◽  
pp. 3877-3883 ◽  
Author(s):  
S. Laperche ◽  
N. Le Marrec ◽  
A. Girault ◽  
F. Bouchardeau ◽  
A. Servant-Delmas ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Early Detection ◽  
Simultaneous Detection ◽  
Hcv Infection ◽  
Core Antigen ◽  
Hcv Core Antigen

scholarly journals PREVALENCE AND GENOTYPING OF HEPATITIS C VIRUS IN HEMODIALYSIS PATIENTS AND EVALUATION OF HCV-CORE ANTIGEN TEST IN SCREENING PATIENTS FOR DIALYSIS IN SANA'A CITY, YEMEN

2019 ◽  
Author(s):  
Samira H Hanash ◽  
Hassan A. Al-Shamahy ◽  
Mohammed Hussein Saleh Bamshmous
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
General Population ◽  
Predictive Power ◽  
Hcv Infection ◽  
Health Concern ◽  
Core Antigen ◽  
Antigen Test ◽  
Genotype 4 ◽  
Hcv Core Antigen

Hepatitis C virus infection is a constant worldwide public health concern. The prevalence of HCV infection is higher in patients on chronic haemodialysis (HD) than in the general population. Despite the control of blood products, hepatitis C virus transmission is still being observed among patients undergoing dialysis. Detection systems for serum HCV antibodies are insensitive in the acute phase because of the long serological window. Direct detection of HCV depends on PCR test but this test is not suitable for routine screening. The objective of this study was to determine prevalence of HCV, genotyping and if HCV core antigen test could be a better alternative to NAT techniques for the diagnosis of HCV infection during the window period and whether the sensitivity for antibody detection is preserved. We screened 159 patients on long-term dialysis by HVC antibodies test, PCR HCV-RNA and HCV core antigen test by commercial tests. The prevalence of HCV was 10.7% (17 patients) and genotype 4 was the most common one (64.7%). The sensitivity of HCV core antigen test was 94.1%, the specificity 100%, the positive predictive power 100%, and the negative predictive power 97.9%. In conclusions; patients on maintenance HD in Yemen have a high prevalence of HCV infection comparing with general population; and genotype 4 is predominant. The performance of serological detection of HCV core antigen was better than that of HCV antibodies test and may be an alternative to nucleic acid amplification technology (NAT) for routine monitoring of patients on chronic dialysis.

scholarly journals Hepatitis C Virus Core Mutations Reduce the Sensitivity of a Fluorescence Enzyme Immunoassay

2000 ◽  
Vol 38 (9) ◽  
pp. 3450-3452 ◽  
Author(s):  
Hajime Tokita ◽  
Gilbert R. Kaufmann ◽  
Mamoru Matsubayashi ◽  
Isao Okuda ◽  
Tsukasa Tanaka ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Enzyme Immunoassay ◽  
Core Region ◽  
Core Antigen ◽  
Nucleotide Sequencing ◽  
Hcv Rna ◽  
Virus Core ◽  
Hcv Core Antigen ◽  
Rna Levels

Four of 107 samples obtained from hepatitis C virus (HCV) carriers showed lower HCV core antigen levels in a fluorescence enzyme immunoassay (FEIA) than expected from corresponding HCV RNA levels. Nucleotide sequencing revealed a mutation in the HCV core region (Thr49Pro) that appears to have reduced the FEIA sensitivity.

scholarly journals Hepatitis C Virus Core Antigen Test in Monitoring of Dialysis Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Gioacchino Li Cavoli ◽  
Carmela Zagarrigo ◽  
Onofrio Schillaci ◽  
Francesca Servillo ◽  
Angelo Tralongo ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Predictive Power ◽  
Hcv Infection ◽  
Health Concern ◽  
Core Antigen ◽  
Antigen Test ◽  
Dialysis Patients ◽  
Hcv Core Antigen ◽  
Routine Monitoring

Hepatitis C virus infection is a persistent worldwide public health concern. The prevalence of HCV infection is much higher in patients on chronic haemodialysis (HD) than in the general population. HCV infection can detrimentally affect patients throughout the spectrum of chronic kidney disease. Despite the control of blood products, hepatitis C virus transmission is still being observed among patients undergoing dialysis. Detection systems for serum HCV antibodies are insensitive in the acute phase because of the long serological window. Direct detection of HCV depends on PCR test but this test is not suitable for routine screening. Recent studies have highlighted the importance of HCV core antigen detection as an alternative to PCR. Few studies exist about the efficacy of HCV core antigen test in dialysis population. We studied the utility of HCV core antigen test in routine monitoring of virological status of dialysis patients. We screened 92 patients on long-term dialysis both by PCR HCV-RNA and HCV core antigen test. The sensitivity of HCVcAg test was 90%, the specificity 100%, the positive predictive power 100%, the negative predictive power 97%, and the accuracy 97%. We think serological detection of HCV core antigen may be an alternative to NAT techniques for routine monitoring of patients on chronic dialysis.

scholarly journals Evaluation of a new enzyme immunoassay for hepatitis C virus(HCV) core antigen with clinical sensitivity approximating that of genomic amplification of HCV RNA

Hepatology ◽  
2000 ◽  
Vol 32 (2) ◽  
pp. 388-393 ◽  
Author(s):  
Eiji Tanaka ◽  
Chiharu Ohue ◽  
Katsumi Aoyagi ◽  
Kenjiro Yamaguchi ◽  
Shintaro Yagi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Enzyme Immunoassay ◽  
Core Antigen ◽  
Hcv Rna ◽  
Genomic Amplification ◽  
Clinical Sensitivity ◽  
Hcv Core Antigen

scholarly journals Evaluation of Hepatitis C Virus Core Antigen Assay in a Resource-Limited Setting in Pakistan

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1354
Author(s):  
Adeel Abid ◽  
Murad Uddin ◽  
Taj Muhammad ◽  
Safia Awan ◽  
Tanya Applegate ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Sensitivity And Specificity ◽  
Resource Limited Setting ◽  
Hcv Infection ◽  
Confirmatory Test ◽  
Core Antigen ◽  
Hcv Rna ◽  
Resource Limited ◽  
Limited Setting

The diagnosis of Hepatitis C virus (HCV) infection can be challenging due to its cost and a lack of access to centralized testing. There is an urgent need to develop simplified HCV testing algorithms. The aim of this study was to evaluate the performance characteristics of a Hepatitis C core antigen (HCVcAg) assay in a decentralized, resource-limited setting. This is a descriptive cross-sectional study from a highly endemic area of Karachi, Pakistan. Between October 2019 and July 2020, subjects aged 12 years and above who screened positive for HCV antibodies were simultaneously tested for HCV RNA (Xpert HCV Viral Load, GeneXpert® IV, Cepheid, France) and HCVcAg (ARCHITECT HCV Ag assay, Abbott® Diagnostics) to confirm active HCV infection. An Abbott ARCHITECT® i1000SR Immunoassay Analyser was installed at a local district hospital as a point-of-care (POC) facility for HCVcAg testing, while samples for HCV RNA were tested in a central lab. Two hundred individuals (mean age 46.4 ± 14.5 years, 71.5% females), who screened positive for HCV antibody, were included in the study. HCV RNA was detected in 128 (64.0%) while HCVcAg was reactive in 119 (59.5%) cases. Performance of the Immunoassay Analyser was excellent with a higher throughput and quicker readout value compared to the GeneXpert System. The sensitivity and specificity of HCVcAg (≥10 fmol/L) at HCV RNA thresholds of ≥12 was 99.1% (95% CI: 95–100%) and 87.6% (95%CI: 78.4–94%). A strong agreement was observed between the HCVcAg assay and HCV RNA. The ARCHITECT HCV Ag assay showed high sensitivity and specificity compared to HCV RNA in a decentralized, resource-limited setting. It can therefore be used as a confirmatory test in HCV elimination programs, particularly for low-income countries such as Pakistan.

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