Insecticidal Activity and Synergistic Effect of Gundelia Tournefortii L. ( Asteraceae : Compositae ) Extracts and Some Pure Constituents on Drosophila Melanogaster Meigen ( Drosophilidae : Diptera )

2015 ◽  
Vol 11 (2) ◽  
pp. 353-366
Author(s):  
Ihab Husni Ghabeish
Keyword(s):  
Drosophila Melanogaster ◽  
Synergistic Effect ◽  
Insecticidal Activity

Organization of the Drosophila melanogaster hsp70 heat shock regulation unit

1987 ◽  
Vol 7 (3) ◽  
pp. 1055-1062
Author(s):  
J Amin ◽  
R Mestril ◽  
P Schiller ◽  
M Dreano ◽  
R Voellmy
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Synergistic Effect ◽  
Consensus Sequence ◽  
Relative Orientation ◽  
Hsp70 Promoter ◽  
Promoter Sequences ◽  
Highly Active ◽  
Sequence Elements ◽  
Five Elements

Expression from the Drosophila melanogaster hsp70 promoter was controlled by a regulatory unit that was composed of two sequence elements that resembled the heat shock consensus sequence. The unit functioned in both orientations and at different distances from downstream promoter sequences. Each element of the unit alone was essentially inactive. Association of two elements resulted in a dramatic increase of transcription from the hsp70 promoter. This synergistic effect was independent of the relative orientation of the elements and, to a large extent, of the distance between them. Duplication of a region containing only one element also yielded a highly active, heat-regulated promoter. Genes with three to five elements were three to four times more active than those with a single regulatory unit.

Search for Bioactive Compounds from Cantharellus cibarius

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Włodzimierz Maria Daniewski ◽  
Witold Danikiewicz ◽  
W. Marek Gołębiewski ◽  
Mirosław Gucma ◽  
Agnieszka Łysik ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Synergistic Effect ◽  
Bioactive Compounds ◽  
Insecticidal Activity ◽  
Methyl Esters ◽  
Preparative Hplc ◽  
Crude Extracts

Ground fruit bodies of Cantharellus cibarius (chanterelle) were extracted with dichloromethane and subjected to CC followed by preparative HPLC, which led to the isolation of glycerol 1,2- and 1,3-dilinoleates and glycerol tridehydrocrepenynate. Extraction of C. cibarius fruit bodies with ethanol or methanol afforded fatty acid ethyl or methyl esters as a result of esterification/transesterification reactions. Insecticidal activity of the isolated glycerides and esters was much lower than that of the crude extracts and chromatographic fractions suggesting a synergistic effect of some of the compounds present in the mixture.

scholarly journals Assessment of Insecticidal Activity of Benzylisoquinoline Alkaloids from Chilean Rhamnaceae Plants against Fruit-Fly Drosophila melanogaster and the Lepidopteran Crop Pest Cydia pomonella

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5094
Author(s):  
Soledad Quiroz-Carreño ◽  
Edgar Pastene-Navarrete ◽  
Cesar Espinoza-Pinochet ◽  
Evelyn Muñoz-Núñez ◽  
Luis Devotto-Moreno ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Insecticidal Activity ◽  
Cydia Pomonella ◽  
Fruit Fly ◽  
Codling Moth ◽  
Ecdysone Receptor ◽  
Action Mechanism ◽  
Benzylisoquinoline Alkaloids ◽  
Relevant Effect ◽  
Feeding Medium

The Chilean plants Discaria chacaye, Talguenea quinquenervia (Rhamnaceae), Peumus boldus (Monimiaceae), and Cryptocarya alba (Lauraceae) were evaluated against Codling moth: Cydia pomonella L. (Lepidoptera: Tortricidae) and fruit fly Drosophila melanogaster (Diptera: Drosophilidae), which is one of the most widespread and destructive primary pests of Prunus (plums, cherries, peaches, nectarines, apricots, almonds), pear, walnuts, and chestnuts, among other. Four benzylisoquinoline alkaloids (coclaurine, laurolitsine, boldine, and pukateine) were isolated from the above mentioned plant species and evaluated regarding their insecticidal activity against the codling moth and fruit fly. The results showed that these alkaloids possess acute and chronic insecticidal effects. The most relevant effect was observed at 10 µg/mL against D. melanogaster and at 50 µg/mL against C. pomonella, being the alteration of the feeding, deformations, failure in the displacement of the larvae in the feeding medium of D. melanogaster, and mortality visible effects. In addition, the docking results show that these type of alkaloids present a good interaction with octopamine and ecdysone receptor showing a possible action mechanism.

scholarly journals In Silico Studies of Lamiaceae Diterpenes with Bioinsecticide Potential against Aphis gossypii and Drosophila melanogaster

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 766
Author(s):  
Gabriela Cristina Soares Rodrigues ◽  
Mayara dos Santos Maia ◽  
Andreza Barbosa Cavalcanti ◽  
Natália Ferreira de Sousa ◽  
Marcus Tullius Scotti ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Insecticidal Activity ◽  
High Probability ◽  
Aphis Gossypii ◽  
Agricultural Products ◽  
Computer Assisted ◽  
In Silico Studies ◽  
Clerodane Diterpenes ◽  
Low Toxicity ◽  
Plant Compounds

Background: The growing demand for agricultural products has led to the misuse/overuse of insecticides; resulting in the use of higher concentrations and the need for ever more toxic products. Ecologically, bioinsecticides are considered better and safer than synthetic insecticides; they must be toxic to the target organism, yet with low or no toxicity to non-target organisms. Many plant extracts have seen their high insecticide potential confirmed under laboratory conditions, and in the search for plant compounds with bioinsecticidal activity, the Lamiaceae family has yielded satisfactory results. Objective: The aim of our study was to develop computer-assisted predictions for compounds with known insecticidal activity against Aphis gossypii and Drosophila melanogaster. Results and conclusion: Structure analysis revealed ent-kaurane, kaurene, and clerodane diterpenes as the most active, showing excellent results. We also found that the interactions formed by these compounds were more stable, or presented similar stability to the commercialized insecticides tested. Overall, we concluded that the compounds bistenuifolin L (1836) and bistenuifolin K (1931), were potentially active against A. gossypii enzymes; and salvisplendin C (1086) and salvixalapadiene (1195), are potentially active against D. melanogaster. We observed and highlight that the diterpenes bistenuifolin L (1836), bistenuifolin K (1931), salvisplendin C (1086), and salvixalapadiene (1195), present a high probability of activity and low toxicity against the species studied.

scholarly journals Organization of the Drosophila melanogaster hsp70 heat shock regulation unit.

1987 ◽  
Vol 7 (3) ◽  
pp. 1055-1062 ◽  
Author(s):  
J Amin ◽  
R Mestril ◽  
P Schiller ◽  
M Dreano ◽  
R Voellmy
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Synergistic Effect ◽  
Consensus Sequence ◽  
Relative Orientation ◽  
Hsp70 Promoter ◽  
Promoter Sequences ◽  
Highly Active ◽  
Sequence Elements ◽  
Five Elements

Expression from the Drosophila melanogaster hsp70 promoter was controlled by a regulatory unit that was composed of two sequence elements that resembled the heat shock consensus sequence. The unit functioned in both orientations and at different distances from downstream promoter sequences. Each element of the unit alone was essentially inactive. Association of two elements resulted in a dramatic increase of transcription from the hsp70 promoter. This synergistic effect was independent of the relative orientation of the elements and, to a large extent, of the distance between them. Duplication of a region containing only one element also yielded a highly active, heat-regulated promoter. Genes with three to five elements were three to four times more active than those with a single regulatory unit.

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