دراسة العوامل المؤثرة في تجذير أصل اللوزيات GF 677 باستخدام تقانة زراعة الأنسجة النباتية = Studies of Factors Affecting Rooting of GF 677 Rootstock by Plant Tissue Culture Technique

2014 ◽  
Vol 10 (1) ◽  
pp. 193-204
Author(s):  
وسيم إسماعيل محسن ◽  
عبد الكريم دقة ◽  
سليم زيد
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Technique ◽  
Factors Affecting ◽  
Tissue Culture Technique

scholarly journals INFLUENCE OF CYTOKININS AND SUCROSE CONCENTRATIONS ON BULBLET CHARACTERS OF IN VITRO HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Dry Weight ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Tissue Culture Technique

The First Part of this study was conducted at the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. and completed at the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum.plantlets were resulted from leaves induced plant lets moved to new MS media supplemented with BA and Kin at 2.0, 1.0, 0.5, 0.0 mg.liter-1 individually or in combination and with or without NAA at 0.5, 0.3, 0.1, 0.0 mg.liter-1 to enhance shoot proliferation. Transferred shoot  from the best proliferation- enhance to stage bulbs formation, MS media supplemented with BA at 6.0, 3.0, 1.5, 0.0 0  mg.liter-1 in addition to sucrose at  90, 60, 300 g.liter-1 with the present of NAA at 0.1 mg.liter-1 to increase bulbs formation, weight, and diameter .Result showed that the best  shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. As for bulbs formation, the results exhibited that MS media supplemented with  6.0  mg.liter-1 BA and 90 g.liter-1 sucrose with the existence of 0.10 mg.liter-1 NAA gave the highest bulbs formation percentage, diameter, and both fresh and dry weight which were 3 bulbs.explant-1, 0.98 cm, and 1.04 and 0.25 g, respectively.

scholarly journals An Overview of Oil Palm Cultivation via Tissue Culture Technique

2021 ◽  
Author(s):  
Siti Khadijah A. Karim
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Oil Palm ◽  
Plant Tissue ◽  
Culture Technique ◽  
Mitigation Measures ◽  
Culture Techniques ◽  
Tissue Culture Technique ◽  
Tissue Culture Techniques

During the last three decades, plant cell, tissue, and organ culture have developed rapidly and become a major biotechnology tool in agriculture, horticulture, forestry, and industry. Many problems in conventional breeding techniques were solved via tissue culture techniques. Plant tissue culture technique permits the growing plants in test tube or closed container in vitro under controlled environment. This technique is devoted to solve two problems: 1) To keep the plant cells free from microbes. 2) To grow the desired plants by providing suitable nutrient medium and other environmental conditions. In this chapter, a review around plant tissue culture techniques that have been reported on oil palm breeding programme will be discussed. It is including the laboratory techniques, advantages and disadvantages of the technique, the problems to produce good and prolific oil palm tissue culture clones and mitigation measures that have been reported to overcome the problems. As a conclusion, this chapter reviews tissue culture techniques that could be used to propagate oil palm clones.

scholarly journals EFEKTIFITAS PENAMBAHAN MEDIA ORGANIK EKSTRAK UBI JALAR (Ipomoea batatas L) PADA PERTUMBUHAN SUBKULTUR ANGGREK Cattleya sp.

2017 ◽  
Vol 4 (1) ◽  
pp. 5
Author(s):  
Shinta Nurdika Meilani ◽  
Septarini Dian Anitasari ◽  
Fatimatuz Zuhro
Keyword(s):  
Tissue Culture ◽  
Sweet Potato ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Ipomoea Batatas ◽  
Economic Value ◽  
Conventional Technique ◽  
Culture Technique ◽  
Leaf Width ◽  
Number Of Leaves

<p align="center"> </p><p><em>Orchids (Chattleya sp) is popular plant which is favorted by peoples and has higt economic value. It’s price Rp. 562,000 / plant. Tissue culture technique is needed to produce this flower quicky than conventional technique. This study have aims to determine the effect of some medium concentration of organic sweet potato (Ipomoea batatas L) on growing Cattleya sp and for knowing the best of concentration on the growth of  Chattleya sp. </em><em>The method used was Completely Random Design (RAL) with four of factors, that is: 0 g / L, 75 g / L, 150 g / L, and 300 g / L. The results were analyzed with Anova test and Duncan test multiple ranger test at the level of 5%. Each treatment was repeated six times. Parameters were observed are the number of new shoots, long leaf, number of leaves, leaf width, long roots, and height of the plant for 5 weeks. Based on the results of analysis showed that the sweet potato extract on 150 g / L concentration give the best results in long root parameter, the concentration of 75 g / L gived the best results to long leaf parameter and concentration of 300 g / L gived the best effect against leaf width. Same concentrations of sweet potato did not give significantly effect to number of new shoots, plantlets height, and number of leaves. </em></p>

scholarly journals In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique

2021 ◽  
Vol 8 (2) ◽  
pp. 244-253
Author(s):  
Akshay Milind Patil ◽  
Pooja Prakash Gunjal ◽  
Dr. Sonali Das
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Health Benefits ◽  
Vitamin B1 ◽  
Scar Tissue ◽  
Culture Technique ◽  
Therapeutic Uses ◽  
Micro Propagation

The multiplication efficacy by bulb is low and the plantlets are more susceptible to disease, therefore, there is a need to develop a protocol for its propagation. Lilium candidum is listed in the saitma prefecture Red Data Book as a critically endangered plant and rescuing information regarding its micro-propagation is rather limited. On this regard, the application of in vitro micropropagtion procedure might help to obtain large numbers of uniform plants of endangered species of Lilium. Dried lilies are a rich source of fiber and also rich in sodium and carbs. Lily bulbs have proteins and starch and also small quantities of iron, calcium, phosphorous, and vitamin B1, B2, C. The health benefits of the lily for the heart are well known on account of the active cardiac glycosides as well as the flavonoids which tend to stimulate the arteries and can cause them to dilute. Another one of the therapeutic uses of the lily flower is in the case of treating burns and preventing the formation of scar tissue. One of the main health benefits of the lily flower is that it helps regulating the heart rate there by allowing the heart to function more efficiently and regular. Having multiple medicinal properties we decided to cultivate Lilium candidum using plant tissue culture so farming can be increased using this cost efficient techniques. In this research, we have studied various Effect of different concentration of BAP and NAA on the initiation of Lilium candidum from bulb and IBA, IAA and NAA on the rooting of shoots of Lilium Candidum.

scholarly journals STUDY THE EFFECT OF CYTOKININS AND AUXINS IN THE COMPOSITION AND PRODUCTION OF IN VITRO PLANTLETS HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Best Response ◽  
Response Profile ◽  
Half Strength

The First Part of this study was conducted in the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. The experiment was then completed in the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum. Explants (Bulbs, Leaves) had been sterilized using four different NaOCL concentrations: % 4.0, 2.0, 1.0, 0.0. After sterilization, explants were cultured on MS media supplemented with BA at four concentrations (2.0, 1.0, 0.5, 0.0 mg.liter-1) and NAA at four concentrations (0.5, 0.3, 0.1, 0. 0 mg.liter-1) to obtain plantlets. Afterwards, plantlets were moved to new MS media supplemented with BA (2.0, 1.0, 0.5, 0.0 mg.liter-1) and with or without four concentrations of NAA (0.5, 0.3, 0.1, 0.0 mg.liter-1) to enhance shoot proliferation. The resulted shoots from the best proliferation-enhance media were divided into two parts the first part was transferred to root-promoting media which also included two experiments where the first experiment was by transferring the shoots to MS media (half and full strength) supplemented with NAA at four concentrations (0.5, 0.3, 0.1, and 0.0  mg.liter-1). The second experiment included the best resulted shoots from the first experiment in addition to (0 and 2 mg.liter-1) actived charcoal to increase root percentage, root count, and root length.The resulted plants were acclimatized using peatmoss/soli mixture at the ratio of 1:1, 2:1, and 1:2 to obtain high surviving ratio. Results showed that the best explant(Bulbs, Leaves) was soaking in 3% NaOCl for 10 min. the results also showed that the leaves gave best response 90% when using MS media supplemented with 1.0 mg.liter-1  BA and 0.3 mg.liter-1 NAA when compared with the bulbs that showed low response profile. Furthermore, the best shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. The best rooting percentage was obtained when culturing the shoots in half strength MS media supplemented with 0.5 mg.liter-1 NAA with the existence of activated charcoal. The surviving percentage reached 95% when using the previously mentionedpeatmoss-soil/mixtures.  

Development of Plant Tissue Culture Profile of Dioscorea deltoidea

Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
M Mujeeb ◽  
M Amir ◽  
AS Nadeem ◽  
M Aqil ◽  
AK Najmi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Dioscorea Deltoidea

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant
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