Comparison of Broad Range 16S rDNA PCR and BACTEC System for Detection of Neonatal Sepsis = مقارنة بين التفاعل التسلسلي البوليميريزي باستخدام 16 اس ريبوسومال د ن أ واسع المجال و جهاز الباكتك لتشخيص تلوث الدم البكتيري في الأطفال حديثي الولا

2014 ◽  
Vol 23 (2) ◽  
pp. 7-12
Author(s):  
Soumaya El-Sayed Hadhoud ◽  
Safaa Mohamad El-Ageery ◽  
Howayda Nabih Fouad Mahran
Keyword(s):  
16S Rdna ◽  
Neonatal Sepsis ◽  
16S Rdna Pcr ◽  
Bactec System

DETECTING MYCOBACTERIUM TUBERCULOSIS RAPIDLY AND MYCOBACTERIUM TUBERCULOSIS STRAINS WITHOUT IS6110 SEQUENCE BY PCR ASSAY

2012 ◽  
pp. 15-19
Author(s):  
Thi Chau Anh Nguyen ◽  
Hoang Bach Nguyen ◽  
Hai Duong Huynh ◽  
Nu Xuan Thanh Le ◽  
Xuan Cuong Le ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
16S Rdna ◽  
False Negative ◽  
Clinical Samples ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Complex Objects ◽  
Method Performance ◽  
Gene Coding ◽  
16S Rdna Pcr

Background: The Nested IS6110 PCR is used for detecting tuberculosis, however IS6110 sequence is not present in the genome of all strains of M.tuberculosis, the result may be false negative. The gene coding 16S ribosome always contains a short sequence specific to M. tuberculosis complex. Objects: Performance of the 16S Real-time PCR to detect M. tuberculosis and combining to the nested IS6110 PCR to determine the rate of Mtb strains without IS6110 from clinical samples. Materials and method: Performance of 16S rDNA PCR by commercial kit of Viet A Inc. for all 480 samples, the samples which were positive with the 16S rDNA PCR were retested in IS6110 PCR assay by in-house kit. Results: The Realtime 16S rDNA PCR detected 258 cases (53.8%) of tuberculosis. There were 3 (1.2 %) M. tuberculosis strains which do not harbor IS6110 sequence in genome. Conclusion: The IS6110 nested PCR can be applied more widely than the 16S rDNA realtime PCR. In case of using IS6110 PCR assay, results may show a low proportion of false negative. Combining 16S rDNA PCR with the IS6110 based PCR allowed detection of deletion of IS6110 sequence in M. tuberculosis isolates.

Isolation of Enterobacter sakazakii from Stable Flies, Stomoxys calcitrans L. (Diptera: Muscidae)†

2006 ◽  
Vol 69 (3) ◽  
pp. 671-673 ◽  
Author(s):  
F. MRAMBA ◽  
A. BROCE ◽  
L. ZUREK
Keyword(s):  
16S Rdna ◽  
Foodborne Pathogen ◽  
Animal Manure ◽  
Stomoxys Calcitrans ◽  
Fecal Coliforms ◽  
Stable Fly ◽  
Enterobacter Sakazakii ◽  
Stable Flies ◽  
16S Rdna Pcr ◽  
Glucose Agar

Enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. Previously, it was suggested that stable flies, Stomoxys calcitrans, were a vector or reservoir of this pathogen. In our study, by means of a culturing approach combined with 16S rDNA PCR–restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in Kansas and Florida. Two stable flies (0.2%) were positive for E. sakazakii. In addition, 411 (44%) stable flies carried bacteria-forming red colonies (presumably enterics) on a violet red bile glucose agar (mean count = 6.4 × 104 CFU per fly), and 120 (13%) stable flies carried fecal coliforms (mean count = 8.7 × 103 CFU per fly). Sequencing of 16S rDNA showed that enterics from violet red bile glucose agar were represented by several genera, including Escherichia, Shigella, Providencia, Enterobacter, Pantoea, Proteus, Serratia, and Morganella. Our study shows that stable flies carry bacteria typically present in animal manure (a developmental site of stable fly larvae), which indicates that the natural reservoir of E. sakazakii is the digestive tract or manure of domestic animals. The low prevalence of E. sakazakii associated with stable flies suggests that stable flies do not play a major role as a reservoir or vector of this pathogen.

scholarly journals Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Arthur Abelian ◽  
Thomas Mund ◽  
Martin D. Curran ◽  
Stuart A. Savill ◽  
Nipa Mitra ◽  
...  
Keyword(s):  
Bacterial Meningitis ◽  
16S Rdna ◽  
Feasibility Study ◽  
Pcr Assay ◽  
16S Rdna Pcr

Analysis of bacterial communities on aging flue-cured tobacco leaves by 16S rDNA PCR–DGGE technology

2007 ◽  
Vol 73 (6) ◽  
pp. 1435-1440 ◽  
Author(s):  
Mingqin Zhao ◽  
Baoxiang Wang ◽  
Fuxin Li ◽  
Liyou Qiu ◽  
Fangfang Li ◽  
...  
Keyword(s):  
16S Rdna ◽  
Bacterial Communities ◽  
Tobacco Leaves ◽  
16S Rdna Pcr

scholarly journals A multicentre study of bacteraemia using a new commercial universal 16S rDNA PCR test

Critical Care ◽  
2010 ◽  
Vol 14 (Suppl 1) ◽  
pp. P49
Author(s):  
S Sakka ◽  
N Wellinghausen ◽  
A Kochem ◽  
C Disque ◽  
H Mühl ◽  
...  
Keyword(s):  
Multicentre Study ◽  
16S Rdna ◽  
16S Rdna Pcr ◽  
Pcr Test

A rapid method for identification of typical Leuconostoc species by 16S rDNA PCR-RFLP analysis

2003 ◽  
Vol 55 (1) ◽  
pp. 295-302 ◽  
Author(s):  
Jichan Jang ◽  
Bongjoon Kim ◽  
Jongho Lee ◽  
Hongui Han
Keyword(s):  
16S Rdna ◽  
Rapid Method ◽  
Rflp Analysis ◽  
16S Rdna Pcr ◽  
Pcr Rflp

scholarly journals A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hellen Ribeiro Martins dos Santos ◽  
Caio Suzart Argolo ◽  
Ronaldo Costa Argôlo-Filho ◽  
Leandro Lopes Loguercio
Keyword(s):  
16S Rdna ◽  
16S Rdna Pcr ◽  
Mock Communities

scholarly journals Identification of Corynebacterium jeikeium from a CAPD patient by 16S rDNA PCR – a case report

2014 ◽  
Vol 14 (S3) ◽  
Author(s):  
Gopalakrishnan Sathya Narayanan ◽  
Radha Vijayaraghavan ◽  
Thangam Menon
Keyword(s):  
Case Report ◽  
16S Rdna ◽  
16S Rdna Pcr ◽  
Capd Patient
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