Histological Evaluation of the Effect of Topical Application of Curcumin Powder and Essential Oil on Skin Wound Healing

2015 ◽  
Vol 27 (3) ◽  
pp. 58-63
Author(s):  
Nidhal Hussein Muhammad ◽  
Ban A. Ghani
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Topical Application ◽  
Skin Wound ◽  
Histological Evaluation ◽  
Skin Wound Healing

Antimicrobially active gelatin/[Mg-Al-CO3]-LDH composite films based on clove essential oil for skin wound healing

2021 ◽  
Vol 27 ◽  
pp. 102169
Author(s):  
Guilherme E. de O. Blanco ◽  
Clóvis W.O. de Souza ◽  
Marcela P. Bernardo ◽  
Martin Zenke ◽  
Luiz H.C. Mattoso ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Composite Films ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Clove Essential Oil

Topical Docosahexaenoic Acid (DHA) Accelerates Skin Wound Healing in Rats and Activates GPR120

2016 ◽  
Vol 18 (4) ◽  
pp. 411-419 ◽  
Author(s):  
Eva L. Arantes ◽  
Nathalia Dragano ◽  
Albina Ramalho ◽  
Daniele Vitorino ◽  
Gabriela F. de-Souza ◽  
...  
Keyword(s):  
Wound Healing ◽  
Docosahexaenoic Acid ◽  
Topical Treatment ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Inflammatory Activity ◽  
Histological Evaluation ◽  
Skin Wound Healing

Background: The development of methods for improving skin wound healing may have an impact on the outcomes of a number of medical conditions. The topical use of polyunsaturated fatty acids (PUFAs) can accelerate skin wound healing through mechanisms that involve, at least in part, the modulation of inflammatory activity. Purpose: We evaluated whether G-protein-coupled receptor 120 (GPR120), a recently identified receptor for docosahexaenoic acid (DHA) with anti-inflammatory activity, is expressed in the skin and responds to topical DHA. Method: Male Wistar rats were submitted to an 8.0-mm wound on the back and were immediately administered a topical treatment of a solution containing 30 μM of DHA once a day. The healing process was photodocumented, and tissues were collected on Days 5, 9, and 15 for protein and RNA analyses and histological evaluation. Results: GPR120 was expressed in the intact skin and in the wound. Keratinocytes expressed the most skin GPR120, while virtually no expression was detected in fibroblasts. Upon DHA topical treatment, wound healing was significantly accelerated and was accompanied by the molecular activation of GPR120, as determined by its association with β-arrestin-2. In addition, DHA promoted a reduction in the expression of interleukin (IL) 1β and an increase in the expression of IL-6. Furthermore, there was a significant increase in expression of transforming growth factor β (TGF-β) and the keratinocyte marker involucrin. Discussion: Topical DHA improved skin wound healing. The activation of GPR120 is potentially involved in this process.

scholarly journals Topical Application of Adelmidrol + Trans-Traumatic Acid Enhances Skin Wound Healing in a Streptozotocin-Induced Diabetic Mouse Model

2018 ◽  
Vol 9 ◽  
Author(s):  
Rosalba Siracusa ◽  
Daniela Impellizzeri ◽  
Marika Cordaro ◽  
Enrico Gugliandolo ◽  
Alessio F. Peritore ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Topical Application ◽  
Diabetic Mouse ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Traumatic Acid

scholarly journals The Effects of Aloe vera Cream on the Expression of CD4+ and CD8+ Lymphocytes in Skin Wound Healing

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yos Adi Prakoso ◽  
Kurniasih
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Aloe Vera ◽  
Skin Wound ◽  
Control Group ◽  
Histopathological Study ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
Group I ◽  
Significant Difference

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4+ and CD8+ lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8+ lymphocytes and increased the epidermal thickness and the expression of CD4+ lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4+/CD8+ lymphocytes in the wound area.

scholarly journals Atropa Belladonna L. Water Extract: Modulator of Extracellular Matrix Formation in Vitro and in Vivo

2012 ◽  
pp. 241-250 ◽  
Author(s):  
P. GÁL ◽  
T. VASILENKO ◽  
I. KOVÁČ ◽  
M. KOSTELNÍKOVÁ ◽  
J. JAKUBČO ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Histological Evaluation ◽  
Control Group ◽  
Atropa Belladonna ◽  
Skin Wound Healing ◽  
Collagen Iii

Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.

Deep skin wound healing potential of lavender essential oil and licorice extract in a nanoemulsion form: Biochemical, histopathological and gene expression evidences

2020 ◽  
Vol 29 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Maryam Kazemi ◽  
Mojgan Mohammadifar ◽  
Esmat Aghadavoud ◽  
Zarichehr Vakili ◽  
Mohammad Hossein Aarabi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Essential Oil ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Licorice Extract

The effects of sodium usnic acid by topical application on skin wound healing in rats

2018 ◽  
Vol 97 ◽  
pp. 587-593 ◽  
Author(s):  
Zhiheng Zhang ◽  
Yu Zheng ◽  
Yue Li ◽  
Hui Bai ◽  
Tianwen Ma ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Usnic Acid ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Evaluation of the alcoholic extract of Dipteryx alata Vogel almonds and bark in skin wound healing in C57BL6 mice

2021 ◽  
Vol 73 (6) ◽  
pp. 1315-1322
Author(s):  
M.C.P. Gouveia ◽  
B.W. Minto ◽  
L.F. Sargi ◽  
R.L. Souza ◽  
J.M. Pazzini ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Healing Process ◽  
Skin Wound ◽  
Bark Extract ◽  
Wound Healing Process ◽  
Alcoholic Extract ◽  
Skin Wound Healing ◽  
Significant Difference ◽  
Dipteryx Alata

ABSTRACT The aim of this study was to evaluate the topical application of alcoholic extracts of Dipteryx alata Vogel almonds and bark in skin wound healing in mice. Fifty-four C57BL/6 mice were equally distributed into three groups: Control, Almond, and Bark. A 9 mm skin fragment was resected from the dorsal region of the animals’ thorax. The wounds were submitted to topical application of base cream (vehicle), 10% hydroalcoholic almond extract, or bark extract twice a day. Macroscopic, histological, and immunohistochemical evaluations were conducted on the 7th, 14th, and 21st postoperative days. No significant difference was observed regarding skin wound area among groups, with the parameter presenting only a temporal effect on healing (p>0.05). The almond and control groups exhibited more intense collagenization than the bark group (p<0.05). Dipteryx alata Vogel showed to be inert in the wound healing process in mice.

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