Bacteriocin Typing of Staphylococcus Aureus Isolated from Different Sources in Ibb City , Yemen

2014 ◽  
Vol 7 (1) ◽  
pp. 25-29
Author(s):  
Fadhl A. Al-Gosha’ah ◽  
Shayma M. Al-Baker ◽  
Khadijah Y. Al-Hetar
Keyword(s):  
Staphylococcus Aureus ◽  
Bacteriocin Typing ◽  
Different Sources

scholarly journals Isolation of Enterococcus Species from Food Sources and Its Antibacterial Activity against Staphylococcus Aureus

2020 ◽  
pp. 3164-3171
Author(s):  
Andalus S. Atiyah ◽  
Marwa H. Alkhafaji
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Dairy Products ◽  
Food Samples ◽  
Food Sources ◽  
Enterococcus Spp ◽  
Microbial Defense ◽  
Prevalent Species ◽  
Different Sources ◽  
By Products

The microbial production of substances that have the ability to inhibit the growth of other microorganisms is possibly the most common defense strategy developed in nature. Microorganisms produce a variable collection of microbial defense systems, which include antibiotics, metabolic by-products, lytic agents, bacteriocins and others. The aim of the present study was to isolate and identify Enterococcus spp. and  its most prevalent species from food samples and determine its antibacterial activity against Staphylococcus aureus isolates. A total of 50 food samples from different sources (dairy products (20 samples) and vegetables and fish (15 samples each)) were collected from different local markets in Baghdad and cultured. Enterococcus spp were isolated from only 32 food samples. E. faecium was the most predominant species which was recovered from 20 samples (62.5 %), 10 dairies, 7 vegetables, and 2 fish. E. faecalis was found in 8 samples (25 %), 5 vegetables and 3 fish.  E. avium was recovered 6.25% as well as E. gallinarium (2 samples for each) Enterococcus avium were all isolated from dairy products but Enterococcus gallinarium one sample isolated from dairies and the other from fish. This study indicates the presence of Enterococcus spp. in the food samples and the ability of these bacteria to produce antibacterial substances which are active against closely related clinical isolates.

scholarly journals The assessment and application of a bacteriocin typing scheme forClostridium perfringens

1985 ◽  
Vol 94 (1) ◽  
pp. 69-79 ◽  
Author(s):  
G. N. Watson
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Computer Programs ◽  
Typing Scheme ◽  
Wide Range ◽  
Bacteriocin Typing ◽  
Different Sources ◽  
Susceptibility Patterns

SUMMARYA collection of 50 bacteriocins was assembled and used to type 802 isolates ofClostridium perfringensfrom food poisoning outbreaks and a variety of other sources. It was found that strains of the same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, and the use of a ‘one difference’ rule is proposed for interpretation of the typing patterns of epidemiologically related strains. Isolates of different serotype or of the same serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns.Two computer programs were developed to assist in the interpretation of bacteriocin typing patterns. Their use showed that related and unrelated strains formed different clusters and enabled a range of the 20 most discriminatory bacteriocins to be selected.Isolates ofC. perfringensfrom a wide range of sources were screened for their ability to produce bacteriocins. A much greater proportion of the strains from food poisoning outbreaks was bacteriocinogenic than were isolates from human and animal infections, various foods and the environment. The relevance of these findings to the occurrence ofC. perfringensfood poisoning is discussed.

scholarly journals Lipolytic activity of Staphylococcus aureus from human wounds, animals, foods, and food-contact surfaces in Brazil

2014 ◽  
Vol 8 (08) ◽  
pp. 1055-1058 ◽  
Author(s):  
Jessica Bezerra Dos Santos Rodrigues ◽  
Taiz Siqueira Pinto ◽  
Cybelle Pereira De Oliveira ◽  
Francisca Inês De Sousa Freitas ◽  
Maria do Socorro Vieira Pereira ◽  
...  
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Lipolytic Activity ◽  
Lipase Production ◽  
Nasal Cavities ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Contact Surfaces ◽  
Different Sources

Introduction: S. aureus is of great importance to public health due to its pathogenicity. This study aimed to evaluate lipase production by S. aureus isolates from different sources. Methodology: Lipolytic activity was determined using Tween-Calcium agar (48 hours; 35°C). Results: Eighty-six percent of the isolates from human wounds were positive for lipase production. The frequencies of isolates positive for lipase production were 33.3% from cow udders, 15.4% from the nasal cavities of cattle, 82.9% from ricotta cheeses, and 100% and 91.7% from meat- and vegetable-contact surfaces, respectively. Conclusion: The production of lipase varied among the isolates according to their source.

scholarly journals THE RELATIONSHIP BETWEEN PRODUCTION OF PROTEIN A, CAPSULE STRUCTURE AND SOME ENZYMES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM INFECTIONS OF HUMAN AND ANIMALS

2021 ◽  
Vol 26 (1) ◽  
pp. 169-174
Author(s):  
Safana, A. S. AL-Taan ◽  
ASMAA, H. Al-Jobori
Keyword(s):  
Staphylococcus Aureus ◽  
Enzyme Production ◽  
Protein A ◽  
Capsule Formation ◽  
Negative Results ◽  
Best Value ◽  
Highly Correlated ◽  
The Relationship ◽  
Different Sources ◽  
Positive Results

Thirty-five isolates of Staphylococcus were isolated out of 100 clinical specimens were collected from different infections of human and animals. These isolates were identified as Staphylococcus aureus, 15 of them isolated from human, 15 from cows and only 5 isolates from poultry. Twenty-five (71.4%) isolates of different sources revealed positive results for the detection of protein A, capsule formation and enzyme production of phosphatase, DNase and haemolysin. Seven (20 %) isolates showed negative results for both protein A and capsule as well as for the production of enzymes while only three (8.5%) isolates were not capsulated but able to produce protein A and the enzymes. During the detection of protein A, capsule structure and production of some enzymes, we found that the best value of pH used was the range of 7-7.5. The present study indicated that the presence of protein A and capsule structure is highly associated with the virulence of isolates, and loss of protein A doesn't necessary mean loss of capsule as well. The ability of the isolates to produce their essential enzymes to induct the infection is highly correlated with the ability to produce protein A, without considering the source of the isolates.

scholarly journals Nosocomial Pneumonia Associated to PVL-Producing Staphylococcus aureus in Children in Benin

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Lamine Baba-Moussa ◽  
Théodora Angèle Ahoyo ◽  
Cécile Le Brun ◽  
Michel Makoutodé ◽  
Karim Dramane ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nosocomial Pneumonia ◽  
Gel Electrophoresis ◽  
Pulmonary Infiltrate ◽  
Pulsed Field Gel Electrophoresis ◽  
Paediatric Unit ◽  
Sick Children ◽  
Paediatric Service ◽  
Underlying Diseases ◽  
Different Sources

We determined the type of toxins produced by Staphylococcus aureus strains and the possible source of contamination during an outbreak of nosocomial pneumonia in a Paediatric service in Benin. Data of 37 patients admitted in the malnourished unit who were diagnosed with pneumonia according to WHO definition and with radiological evidence of a pulmonary infiltrate were collected within six weeks. Pneumonia was further confirmed by the identification of corresponding pneumonia-related S. aureus. Samples were also collected from hospital personnel, the environment, and the food fed to the patients. Pulsed field gel electrophoresis (PFGE) was used to compare the bacterial profile from different sources. Among the 37 patients admitted during this period, 17 developed pneumonia and 10 were associated with S. aureus strains. Nine patients infected with S. aureus PVL-producing strains had underlying diseases and developed an acute multilobar pneumonia, which was fulminating and rapidly became fatal in all except the oldest child. Most of the isolates found on sick children were similar to those from special nutrients consumed by children and on the personnel at the hospital. The highly probable relationships between children contamination and isolate presence in the special nutrient underline the rapid and disastrous dissemination of some PVL-producing isolates in this paediatric unit.

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