Serological versus Antigen Detection Methods for Giardia Duodenalis Diagnosis

2014 ◽  
Vol 44 (3) ◽  
pp. 709-718 ◽  
Author(s):  
M. Bashir ◽  
A. Farid ◽  
I. Rabia
Keyword(s):  
Giardia Duodenalis ◽  
Antigen Detection ◽  
Detection Methods ◽  
Versus Antigen

scholarly journals The limitation of circulating Aspergillus antigen detection methods for BMT recipients

1998 ◽  
Vol 22 (8) ◽  
pp. 832-833 ◽  
Author(s):  
M Kami ◽  
Y Tanaka ◽  
S Ogawa ◽  
Y Kanda ◽  
H Honda ◽  
...  
Keyword(s):  
Antigen Detection ◽  
Detection Methods ◽  
Aspergillus Antigen

scholarly journals Comparison of three antigen detection methods for diagnosis and therapeutic monitoring of malaria: a field study from southern Vietnam

2002 ◽  
Vol 7 (4) ◽  
pp. 304-308 ◽  
Author(s):  
Nguyen Mai Huong ◽  
Timothy M. E. Davis ◽  
Sean Hewitt ◽  
Nguyen Van Huong ◽  
Tran Thi Uyen ◽  
...  
Keyword(s):  
Field Study ◽  
Antigen Detection ◽  
Detection Methods ◽  
Therapeutic Monitoring ◽  
Southern Vietnam

Intestinal parasites of gray wolves (Canis lupus) in northern and western Canada

2016 ◽  
Vol 94 (9) ◽  
pp. 643-650 ◽  
Author(s):  
Janna M. Schurer ◽  
Michael Pawlik ◽  
Anna Huber ◽  
Brett Elkin ◽  
H. Dean Cluff ◽  
...  
Keyword(s):  
Canis Lupus ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Parasite Fauna ◽  
Detection Methods ◽  
Taenia Hydatigena ◽  
Gray Wolves ◽  
Wide Range ◽  
Parasite Spillover ◽  
Diphyllobothrium Latum

Gray wolves (Canis lupus L., 1758) are mobile opportunistic predators that can be infected by a wide range of parasites, with many acquired via predator–prey relationships. Historically, many of these parasites were identified only to genus or family, but genetic tools now enable identification of parasite fauna to species and beyond. We examined 191 intestines from wolves harvested for other purposes from regions in the Northwest Territories, British Columbia, Saskatchewan, and Manitoba. Adult helminths were collected from intestinal contents for morphological and molecular identification, and for a subset of wolves, fecal samples were also analyzed to detect helminth eggs and protozoan (oo)cysts. Using both detection methods, we found that 83% of 191 intestines contained one or more parasite species, including cestodes (Taenia spp., Echinococcus spp., and Diphyllobothrium sp.), nematodes (Uncinaria stenocephala Railliet, 1884, Trichuris spp., Physaloptera spp., and Toxascaris leonina (von Linstow, 1902)), a trematode (Alaria sp.), and protozoa (Sarcocystis spp., Giardia sp., and Cryptosporidium spp.). Molecular characterization identified one species of Diphyllobothrium (Diphyllobothrium latum (L., 1758) Cobbold, 1858), three species of Taenia (Taenia krabbei Moniez, 1879, Taenia hydatigena Pallas, 1766, and Taenia multiceps Leske, 1786), and two Giardia duodenalis (Davaine) Deschiens, 1921 assemblages (B and C). These results demonstrate the diverse diet of wolves and illustrate the possibility of parasite spillover among wildlife, domestic animals, and people.

Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods

Parasitology ◽  
1999 ◽  
Vol 117 (7) ◽  
pp. 41-57 ◽  
Author(s):  
J. V. HAMILTON ◽  
M. KLINKERT ◽  
M. J. DOENHOFF
Keyword(s):  
Recombinant Dna ◽  
Infection Intensity ◽  
Public Health Problem ◽  
Antigen Detection ◽  
Detection Methods ◽  
Antibody Detection ◽  
Native Form ◽  
Keyhole Limpet Haemocyanin ◽  
Advantages And Disadvantages ◽  
Wide Range

Schistosomiasis remains a serious world-wide public health problem with a still unfulfilled need for routine cost-effective methods of diagnosis. Such methods are required not only for people in endemic areas, but increasingly for tourists who may have become infected during visits to such places. This article reviews the wide range of immunoassays and antigenic preparations that have been shown to have potential for diagnosis of schistosomiasis by the indirect method of antibody detection. Antigens in native form derived from cercariae, adult worms and eggs are considered, as well as schistosome antigens produced by recombinant DNA technology and the schistosome cross-reactive antigen, keyhole limpet haemocyanin (KLH). Respective advantages and disadvantages of antibody detection, circulating antigen detection and parasitological methods of diagnosis are analysed. It is suggested that due to the relative insensitivity of both parasitology and antigen detection, antibody detection methods could find increasing use in situations of low infection intensity.

Comparison of three major antigen detection methods for the diagnosis of Group B streptococcal sepsis in neonates

1983 ◽  
Vol 2 (6) ◽  
pp. 432-435 ◽  
Author(s):  
AYSER CHALABI HAMOUDI ◽  
MARIO J. MARCON ◽  
HAROLD J. CANNON ◽  
RICHARD E. MCCLEAD
Keyword(s):  
Antigen Detection ◽  
Detection Methods ◽  
Major Antigen ◽  
Streptococcal Sepsis ◽  
Group B

scholarly journals Comparison of Escherichia coli O157:H7 Antigen Detection in Stool and Broth Cultures to That in Sorbitol-MacConkey Agar Stool Cultures

2000 ◽  
Vol 38 (9) ◽  
pp. 3404-3406 ◽  
Author(s):  
Jennifer R. Stapp ◽  
Srdjan Jelacic ◽  
Yoo-Lee Yea ◽  
Eileen J. Klein ◽  
Marc Fischer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antigen Detection ◽  
Detection Methods ◽  
Broth Culture ◽  
Agar Culture ◽  
E Coli ◽  
Direct Testing ◽  
Stool Specimens ◽  
Stool Cultures ◽  
Test Result

We evaluated the Meridian IC-STAT direct fecal and broth culture antigen detection methods with samples from children infected withEscherichia coli O157:H7 and correlated the antigen detection results with the culture results. Stools of 16 children who had recently had stool cultures positive for this pathogen (population A) and 102 children with diarrhea of unknown cause (population B) were tested with the IC-STAT device (direct testing). Fecal broth cultures were also tested with this device (broth testing). The results were correlated to a standard of the combined yield from direct culture of stools on sorbitol-MacConkey (SMAC) agar and culture of broth on SMAC agar. Eleven (69%) of the population A stool specimens yieldedE. coli O157:H7 when plated directly on SMAC agar. Two more specimens yielded this pathogen when the broth culture was similarly plated. Of these 13 stool specimens, 8 and 13 were positive by direct and broth testing (respective sensitivities, 62 and 100%). Compared to the sensitivity of a simultaneously performed SMAC agar culture, the sensitivity of direct testing was 73%. Three (3%) of the population B stool specimens contained E. coli O157:H7 on SMAC agar culture; one and three of these stool specimens were positive by direct and broth testing, respectively. The direct and broth IC-STAT tests were 100% specific with samples from children from population B. Direct IC-STAT testing of stools is rapid, easily performed, and specific but is insufficiently sensitive to exclude the possibility of infection with E. coli O157:H7. Performing the IC-STAT test with a broth culture increases its sensitivity. However, attempts to recover E. coli O157:H7 by culture should not be abandoned but, rather, should be increased when the IC-STAT test result is positive.

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