scholarly journals Zmiany właściwości antyoksydacyjnych ekstraktów z liści werbeny cytrynowej (Lippia citriodora (Palau) Kunth) w trakcie przechowywania

HERBALISM ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 119-124
Author(s):  
Małgorzata Stryjecka ◽  
Anna Kiełtyka-Dadasiewicz
Keyword(s):  

Oznaczono zmiany zawartości polifenoli ogółem i aktywność antyoksydacyjną metodą z DPPH oraz FRAP ekstraktów: metanolowych, etanolowych, wodnych oraz acetonowych, otrzymanych z werbeny cytrynowej (Lippia citriodora (Palau) Kunth.) w czasie ich przechowywania (3, 6, 9 i 12 miesięcy). Największą zawartość polifenoli ogółem zanotowano w ekstrakcie metanolowym, najniższą zaś w wyciągu acetonowym. Ekstrakt metanolowy charakteryzował się największą aktywnością antyoksydacyjną, oznaczoną zarówno metodą z DPPH, jak i FRAP. Wydłużenie czasu przechowywania wszystkich ekstraktów, niezależnie od czynnika ekstrahującego, powodowało spadek zarówno zawartości polifenoli ogółem, jak i aktywności antyoksydacyjnej.

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 875
Author(s):  
Katerina Spyridopoulou ◽  
Tamara Aravidou ◽  
Evangeli Lampri ◽  
Eleni Effraimidou ◽  
Aglaia Pappa ◽  
...  

Lippia citriodora is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of L. citriodora oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO’s pro-apoptotic potential with the Annexin-PI method. The LCO’s anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential.


2019 ◽  
Vol 7 (12) ◽  
pp. 3986-3992
Author(s):  
Javier Rúa ◽  
Iván López‐Rodríguez ◽  
Javier Sanz ◽  
Pilar Valle Fernández ◽  
Maria del Camino Garcia ◽  
...  

2009 ◽  
Vol 33 (spe) ◽  
pp. 1853-1860 ◽  
Author(s):  
André Costa da Silva ◽  
Nilza de Lima Pereira Sales ◽  
Alisson Vinícius de Araújo ◽  
Cecílio Frois Caldeira Júnior

Neste trabalho, objetivou-se avaliar o efeito de extratos e óleos essenciais de quatorze plantas medicinais e ou nativas, conhecidas popularmente no Norte de Minas Gerais, sobre a germinação de esporos e crescimento micelial do fungo Colletotrichum gloeosporioides. Foram utilizados no teste de inibição do crescimento micelial, os extratos aquosos de alecrim (Rosmarinus officinalis), erva baleeira (Cordia verbenacea), joá (Solanum sisymbriifolium), quebra-pedra (Phyllanthus corcovadensis), erva botão (Eclipta alba) e açafrão da índia (Curcuma longa) obtidos por meio do processo de infusão; óleo essencial de alecrim de vargem (Família Lamiaceae), alecrim pimenta (Lippia sidoides), alfavaca cravo (Ocimum gratissimum), lippia (Lippia citriodora), goiaba branca (Psidium guajava), capim santo (Cymbopogon citratus); óleo resina de copaíba (Copaifera langsdorffi); hidrolatos de alecrim de vargem, goiaba vermelha (Psidium guajava), lippia, capim santo, goiaba branca, alfavaca cravo; manipueira de (Manihot esculenta). Também foram testados esses mesmos hidrolatos e os óleos essenciais na germinação dos esporos do fungo. Todos os tratamentos foram realizados in vitro, cultivando-se o fungo em meio de cultura BDA acrescido de 100μL dos compostos vegetais. No teste de inibição da germinação, todos os óleos essenciais impediram a germinação do fungo. No entanto, os hidrolatos não tiveram esse efeito. No teste de crescimento micelial, os óleos essenciais de todas as plantas inibiram completamente o crescimento do fungo, exceto o óleo da goiaba branca. Os extratos aquosos, a manipueira, o óleo resina e os hidrolatos, também não foram eficientes sobre o crescimento do patógeno. Esses resultados indicam o potencial antifúngico de alguns óleos essenciais de plantas medicinais.


Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 279
Author(s):  
Javier Marhuenda ◽  
Silvia Perez-Piñero ◽  
Desirée Victoria-Montesinos ◽  
María Salud Abellán-Ruiz ◽  
Nuria Caturla ◽  
...  

The authors wish to make the following correction to this paper [...]


2018 ◽  
Vol 109 ◽  
pp. 213-222 ◽  
Author(s):  
Francisco Javier Leyva-Jiménez ◽  
Jesús Lozano-Sánchez ◽  
Isabel Borrás-Linares ◽  
David Arráez-Román ◽  
Antonio Segura-Carretero

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 324 ◽  
Author(s):  
Noelia Sánchez-Marzo ◽  
Jesús Lozano-Sánchez ◽  
María de la Luz Cádiz-Gurrea ◽  
María Herranz-López ◽  
Vicente Micol ◽  
...  

Over the last few years, people have been concerned about the narrow relationship between nutrition and health leading to an increasing demand of nutraceutical products and functional food. Lemon verbena (Lippia citriodora Kunth) has been traditionally used for respiratory, digestive, and muscular diseases, showing effects that are promoted by the antioxidant activity of its phytoconstituents. The antioxidant power of several lemon verbena extracts has been tested but its isolated compounds activity has not been described. The aim of the present work was to isolate phytochemicals from a commercial lemon verbena extract through a semi-preparative high-performance liquid chromatography approach for further evaluation of its individual antioxidant activity using three different methods. The structure-antioxidant activity relationships revealed the influence of substitutions in the strong antioxidant power exerted by glycosylated phenylpropanoids, in contrast to the low antioxidant capacity showed by iridoids. Development of enriched extracts in these compounds could lead to greater antioxidant effects and improved functional ingredients to prevent chronic diseases.


Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 765
Author(s):  
Francisco Javier Leyva-Jiménez ◽  
Maria Letizia Manca ◽  
Maria Manconi ◽  
Carla Caddeo ◽  
José Antonio Vázquez ◽  
...  

Phytochemicals from Lippia citriodora leaves were extracted by applying an innovative technology based on the use of microwaves, which represents an alternative method to extract bioactive substances. The obtained extract was incorporated into phospholipid vesicles in order to promote the antioxidant effect of the bioactive molecules present in L. citriodora extract. The extract was analyzed by High Performance Liquid Chromatography coupled to Time-Of-Flight mass spectrometer by electrospray (HPLC-ESI-TOF-MS) and different phytochemicals were detected and quantified. The whole extract was incorporated in liposomes, glycerosomes (liposomes modified with glycerol) and propylene glycol-containing vesicles (PG-PEVs). Moreover, a biopolymer obtained from fish by-product, that is Thunnus albacares skin, was added to improve the bioactivity of the formulations. The in vitro biocompatibility and the antioxidant efficacy of the extract in solution or loaded in the vesicles were tested in primary mouse embryonic fibroblasts (3T3). The results showed the superior bioactivity of the vesicle formulations over the aqueous solution of the extract, which points to an interesting strategy for the treatment of skin disorders.


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