In Vitro Induction of Potential Primordial Germ Cells from Mouse Embryonic Stem Cells by Culture with Undifferentiated Gonadal Cells

2008 ◽  
Vol 25 (1) ◽  
pp. 37-43
Author(s):  
Ikuo Tomioka ◽  
Yuji Honma ◽  
Hiroshi Sasada ◽  
Eimei Sato
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
In Vitro Induction

Collagen-alginate microspheres as a 3D culture system for mouse embryonic stem cells differentiation to primordial germ cells

Biologicals ◽  
2017 ◽  
Vol 48 ◽  
pp. 114-120 ◽  
Author(s):  
Vahid Mansouri ◽  
Mohammad Salehi ◽  
Mir davood Omrani ◽  
Zahra Niknam ◽  
Abdolreza Ardeshirylajimi
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
3D Culture ◽  
Mouse Embryonic Stem Cells ◽  
3D Culture System

scholarly journals Germ cells from mouse and human embryonic stem cells

Reproduction ◽  
2006 ◽  
Vol 132 (5) ◽  
pp. 699-707 ◽  
Author(s):  
Behrouz Aflatoonian ◽  
Harry Moore
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Endocrine Disrupting Chemicals ◽  
Embryonic Stem ◽  
Founder Population ◽  
Testicular Dysgenesis Syndrome

Mammalian gametes are derived from a founder population of primordial germ cells (PGCs) that are determined early in embryogenesis and set aside for unique development. Understanding the mechanisms of PGC determination and differentiation is important for elucidating causes of infertility and how endocrine disrupting chemicals may potentially increase susceptibility to congenital reproductive abnormalities and conditions such as testicular cancer in adulthood (testicular dysgenesis syndrome). Primordial germ cells are closely related to embryonic stem cells (ESCs) and embryonic germ (EG) cells and comparisons between these cell types are providing new information about pluripotency and epigenetic processes. Murine ESCs can differentiate to PGCs, gametes and even blastocysts – recently live mouse pups were born from sperm generated from mESCs. Although investigations are still preliminary, human embryonic stem cells (hESCs) apparently display a similar developmental capacity to generate PGCs and immature gametes. Exactly how such gamete-like cells are generated during stem cell culture remains unclear especially as in vitro conditions are ill-defined. The findings are discussed in relation to the mechanisms of human PGC and gamete development and the biotechnology of hESCs and hEG cells.

The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells

Biologicals ◽  
2018 ◽  
Vol 56 ◽  
pp. 39-44 ◽  
Author(s):  
Neda Eskandari ◽  
Meysam Hassani Moghaddam ◽  
Mohammad Ali Atlasi ◽  
Javad Amini Mahabadi ◽  
Aliakbar Taherian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genes Expression

scholarly journals Gamete derivation from embryonic stem cells, induced pluripotent stem cells or somatic cell nuclear transfer-derived embryonic stem cells: state of the art

2015 ◽  
Vol 27 (1) ◽  
pp. 89 ◽  
Author(s):  
Charles A. Easley ◽  
Calvin R. Simerly ◽  
Gerald Schatten
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Induced Pluripotent ◽  
Normal Offspring

Generating gametes from pluripotent stem cells (PSCs) has many scientific justifications and several biomedical rationales. Here, we consider several strategies for deriving gametes from PSCs from mice and primates (human and non-human) and their anticipated strengths, challenges and limitations. Although the ‘Weismann barrier’, which separates the mortal somatic cell lineages from the potentially immortal germline, has long existed, breakthroughs first in mice and now in humans are artificially creating germ cells from somatic cells. Spermatozoa with full reproductive viability establishing multiple generations of seemingly normal offspring have been reported in mice and, in humans, haploid spermatids with correct parent-of-origin imprints have been obtained. Similar progress with making oocytes has been published using mouse PSCs differentiated in vitro into primordial germ cells, which are then cultured after xenografting reconstructed artificial ovaries. Progress in making human oocytes artificially is proving challenging. The usefulness of these artificial gametes, from assessing environmental exposure toxicity to optimising medical treatments to prevent negative off-target effects on fertility, may prove invaluable, as may basic discoveries on the fundamental mechanisms of gametogenesis.

scholarly journals Single Cell Analysis Facilitates Staging of Blimp1-Dependent Primordial Germ Cells Derived from Mouse Embryonic Stem Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28960 ◽  
Author(s):  
John J. Vincent ◽  
Ziwei Li ◽  
Serena A. Lee ◽  
Xian Liu ◽  
Marisabel O. Etter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Germ Cells ◽  
Single Cell Analysis ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cell Analysis
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