scholarly journals Basic Study of Assisted Hatching Using Mouse Embryo. Comparison of the Effects with Partial Zona Dissection, Zona Drilling and Zona Thinning.

1996 ◽  
Vol 13 (1) ◽  
pp. 30-38
Author(s):  
Hiroyuki Yazawa ◽  
Kazuhiko Hoshi ◽  
Kaoru Yanagida ◽  
Kazuo Suzuki ◽  
Akira Sato
Keyword(s):  
Mouse Embryo ◽  
Assisted Hatching ◽  
Basic Study ◽  
Partial Zona Dissection ◽  
Zona Drilling

scholarly journals Assisted hatching by zona drilling of human embryos in women of advanced age

1997 ◽  
Vol 12 (2) ◽  
pp. 317-320 ◽  
Author(s):  
D. Bider ◽  
A. Livshits ◽  
M. Yonish ◽  
Z. Yemini ◽  
S. Mashiach ◽  
...  
Keyword(s):  
Advanced Age ◽  
Human Embryos ◽  
Assisted Hatching ◽  
Zona Drilling

Laser micromanipulation in the mouse embryo: a novel approach to zona drilling

1992 ◽  
Vol 57 (6) ◽  
pp. 1337-1341 ◽  
Author(s):  
Graciela B. Blanchet ◽  
Jeffrey B. Russell ◽  
Curtis R. Fincher ◽  
Marc Portmann
Keyword(s):  
Mouse Embryo ◽  
Novel Approach ◽  
Zona Drilling ◽  
Laser Micromanipulation

28 IMPROVED HATCH RATE AFTER PARTIAL DISSECTION OF ZONA PELLUCIDA IN CLONED PIG EMBRYO

2017 ◽  
Vol 29 (1) ◽  
pp. 121
Author(s):  
Y. J. Kim ◽  
K. S. Ahn ◽  
S. M. Park ◽  
B. C. Lee ◽  
H. Shim ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Nuclear Transfer ◽  
Embryo Implantation ◽  
Control Group ◽  
Assisted Hatching ◽  
Mammalian Embryo ◽  
Animal Cloning ◽  
Hatching Process ◽  
Partial Zona Dissection

For last 20 years, the efficiency of animal cloning has remained extremely low, despite many attempts to improve it. Although nuclear transfer experiments have been almost optimized, artificial holes are inevitably made in the zona pellucida (ZP) during nuclear transfer experiments, such as enucleation of maternal genome or injection of nuclear donor. Hatching from the ZP is a prerequisite for mammalian embryo implantation, and the condition of the ZP has a lot of influence on hatching. The present studies were performed to investigate the effects of artificial holes in the ZP, because of the nuclear transfer procedure, on hatching of clone embryos in pigs. All experiments were done in triplicate. Statistical analysis was performed using SPSS statistical software (SPSS Inc., Chicago, IL, USA). First, we made a slit in the ZP of porcine parthenote that was identical to the artificial holes of nuclear transfer experiment and compared in vitro development of Day 7 embryos with control group with intact ZP. Of slit blastocysts, 89.9% (80/89) were trapped at a slit, which looked like typical figure-eight shape, and did not complete the hatching process until Day 8, though 68.8% (64/93) of control blastocysts completed the hatching at Day 7. Then, to solve these abnormal hatchings caused by a slit in the ZP, we applied partial zona dissection (PZD) to porcine clone embryos and compared the hatching process with that of conventional clone embryos. Contrary to conventional clone blastocysts that were trapped at slit in the ZP (91.4%; 43/47), 89.5% (60/67) of clone blastocysts in PZD group were preferentially hatched through dissected hole at Day 7. These results suggest that trapping of conventional clone blastocyst in a slit of the ZP could be avoided by PZD. Through this study, we demonstrated that a slit in the ZP would hinder a blastocyst from hatching from the ZP and that partial dissection at the ZP could help clone blastocyst to hatch without trapping at a slit in the ZP. This assisted hatching in clone embryos would be useful for the successful hatching of clone blastocysts with a capacity of full-term development, so that the efficiency of animal cloning might be improved.

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