Comparative Experimental Evaluation of the Effectiveness of Composition and PTFE Prostheses in Plastic Hiatal Hernia

10.12737/9088 ◽  
2015 ◽  
Vol 22 (1) ◽  
pp. 101-106
Author(s):  
Пряхин ◽  
A. Pryakhin ◽  
Калинина ◽  
E. Kalinina ◽  
Кулаев ◽  
...  
Keyword(s):  
Experimental Study ◽  
Connective Tissue ◽  
Hiatal Hernia ◽  
Postoperative Period ◽  
Collagen Fibers ◽  
Synthetic Material ◽  
Operative Complications ◽  
Morphological Picture ◽  
Ptfe Prosthesis ◽  
Orderly Arrangement

The purpose of this study was to determine the optimal synthetic material for prosthetic hiatal hernia. Materials and methods. Experimental study was carried out in 24 adult rabbits of different sexes. In the ex-periment prosthetic hernioplasty hiatal hernia was simulated. The animals were divided into two groups: 12 hernioplasty with implantation of a monofilament macroporous lightweight composite prosthesis (50% polypropylene, 50% monocryl) and 12 hernioplasty with implantation of a microporous prosthesis from polytetrafluoroethylene PTFE. Comparative analysis of reparative processes in the implantation of endoprosthesis was carried out in the 7th, 30th, 90th and 180th postoperative day. Results. Intra-operative complications in the groups didn’t registered. In the postoperative period after implanta-tion of the composite prosthesis in all periods, the complication wasn’t identified. There are the complications in 3 cases (25%) of the use of PTFE prosthesis in the late postoperative. One patient was found to displacement of the implant on 90th day of the experiment, and 2 observations - cigar-shaped deformation of the prosthesis on the 90th and 180th days of the postoperative period. Feature of the morphological picture in the implantation of the prosthesis PTFE was a large amount of thick convoluted collagen fibers, not germinating implant. The implant is made of composite material, was surrounded by a thin, soft fiber, uniform layer of mature connective tissue with an orderly arrangement of collagen fibers sprouting prosthesis. Conclusion. It was established that the most suitable implant for plastic hiatal hernia is a composite prosthesis as firmly fixed to the diaphragm germinating its connective tissue and has no tendency to shift. The implantation of PTFE prosthesis has a more favorable course of the wound process, however, the implant doesn´t invade connective tissue and is encapsulated with the formation of rough scar.

scholarly journals The Symmetric 3D Organization of Connective Tissue around Implant Abutment: A Key-Issue to Prevent Bone Resorption

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Connective Tissue ◽  
Inflammatory Cell ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Collagen Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Oral Epithelium ◽  
Implant Abutment

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.

Efficacy of a Proton Pump Inhibitor Given in the Early Postoperative Period to Relieve Symptoms of Hiatal Hernia After Open Heart Surgery

Surgery Today ◽  
2006 ◽  
Vol 36 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mitsumasa Hata ◽  
Motomi Shiono ◽  
Hisakuni Sekino ◽  
Hidekazu Furukawa ◽  
Akira Sezai ◽  
...  
Keyword(s):  
Proton Pump Inhibitor ◽  
Hiatal Hernia ◽  
Proton Pump ◽  
Postoperative Period ◽  
Heart Surgery ◽  
Open Heart Surgery ◽  
Early Postoperative Period ◽  
Open Heart

scholarly journals Palatal Wound Healing with Primary Intention in a Rat Model—Histology and Immunohistomorphometry

Medicina ◽  
2020 ◽  
Vol 56 (4) ◽  
pp. 200 ◽  
Author(s):  
Liat Chaushu ◽  
Marina Rahmanov Gavrielov ◽  
Gavriel Chaushu ◽  
Marilena Vered
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Physical Properties ◽  
Rat Model ◽  
Single Incision ◽  
Epithelial Differentiation ◽  
Collagen Fibers ◽  
Surgical Trauma ◽  
Inflammatory Infiltration ◽  
Stromal Collagen

Background and objectives: Subepithelial connective tissue graft (SCTG) from the palate has been considered as the “gold standard” for the treatment of deep gingival recessions. A single-incision technique was reported to allow primary wound healing. A palatal single incision was performed in a rat model. The present study assessed the histology and histomorphometry of palatal wound healing following surgical closure with primary intention. Materials and Methods: Twenty-six 6-month-old male Wistar rats weighing 427–650 g. An incision was made on the maxillary palate. A full thickness flap was raised palatally, and then repositioned and sutured. Two experimental groups: S—Study group, I—Intact control group. Half of the animals were sacrificed 7 days and the remaining 14 days postoperatively. Outcome parameters included—epithelial gap; inflammatory infiltration; vascular fraction, expression of myofibroblasts and stem cell markers within the oral epithelium and stromal cells and physical properties of stromal collagen fibers. Investigations were performed at two time-points (7 and 14 days) during the wound healing process. Results: The epithelial gap closed completely after 14 days. The inflammatory reaction and vascular fraction were relatively low. Surgical trauma downregulated the expression of cytokeratin (CK) 14 and CK 15, which returned to normal after 14 days. Epithelial differentiation was mediated through upregulation of connective tissue sex- determining-region-Y-box2 (SOX2). Epithelial SOX2, CD34, alpha smooth muscle actin (αSMA) and physical properties of stromal collagen fibers were not influenced by the surgical trauma. Conclusions: Surgical trauma followed by palatal wound healing with primary intention in a rat model heals within 14 days. It induces minimal inflammatory infiltration and vascular proliferation. Epithelization is exerted through promotion of epithelial differentiation from stem cells by connective tissue SOX2.

scholarly journals Changes of orbital connective tissue after bimatoprost injection. Experimental study in rats

2016 ◽  
Vol 75 ◽  
Author(s):  
Nilson Lopes da Fonseca Junior ◽  
Giuliana Petri ◽  
Juliana Mora Veridiano ◽  
José Ricardo Carvalho Lima Rehder
Keyword(s):  
Experimental Study ◽  
Connective Tissue ◽  
Orbital Connective Tissue

scholarly journals Fibronectin in rat heart: a link between cardiac myocytes and collagen.

1984 ◽  
Vol 32 (4) ◽  
pp. 383-388 ◽  
Author(s):  
G G Ahumada ◽  
J E Saffitz
Keyword(s):  
Connective Tissue ◽  
Cardiac Myocytes ◽  
Rat Heart ◽  
Collagen Fibers ◽  
Antibody Technique ◽  
Adhesion Properties ◽  
Double Antibody ◽  
Rat Hearts ◽  
Contraction And Relaxation ◽  
And Control

Fibronectin, a glycoprotein that binds to collagen and modifies the adhesion properties and motility of cells in culture, is present in the interstitium of rat hearts. To localize fibronectin more precisely and to assess its relationship to the myocyte and to connective tissue elements, we employed a double antibody technique to label myocardial fibronectin with electron-dense ferritin to permit an ultrastructural analysis. Fibronectin was found to be associated with collagen, and in some cases appeared to link collagen fibers. Fibronectin was also found inserted along the surfaces of cardiac myocytes, connecting these cells to perimyocytic collagen. These ultrastructural relationships imply that fibronectin is a major component of the myocardial interstitium, and may affect myocardial compliance and control the motion of myocytes during the contraction and relaxation of the heart.

scholarly journals ULTRASTRUCTURAL STUDIES ON THE LYMPHATIC ANCHORING FILAMENTS

1968 ◽  
Vol 36 (1) ◽  
pp. 129-149 ◽  
Author(s):  
L. V. Leak ◽  
J. F. Burke
Keyword(s):  
Connective Tissue ◽  
Extracellular Space ◽  
Collagen Fibers ◽  
Capillary Wall ◽  
Surrounding Tissue ◽  
Elastic Fibers ◽  
Lymphatic Capillary ◽  
Ultrastructural Studies ◽  
Outer Leaflet ◽  
Tissue Area

The fine structure of the lymphatic capillary and the surrounding tissue areas was investigated. Instead of a continuous basal lamina (basement membrane) surrounding the capillary wall, these observations revealed the occurrence of numerous fine filaments that insert on the outer leaflet of the trilaminar unit membrane of the lymphatic endothelium. These filaments appear as individual units, or they are aggregated into bundles that are disposed parallel to the long axis of the lymphatic capillary wall and extend for long distances into the adjoining connective tissue area among the collagen fibers and connective tissue cells. The filaments measure about 100 A in width and have a hollow profile. They exhibit an irregular beaded pattern along their long axis and are densely stained with uranyl and lead. These filaments are similar to the microfibrils of the extracellular space and the filaments observed in the peripheral mantle of the elastic fibers. Infrequently, connections between these various elements are observed, suggesting that the lymphatic anchoring filaments may also contribute to the filamentous units of the extracellular space. It is suggested that these lymphatic anchoring filaments connect the small lymphatics to the surrounding tissues and represent the binding mechansim that is responsible for maintaining the firm attachment of the lymphatic capillary wall to the adjoining collagen fibers and cells of the connective tissue area.

scholarly journals Interposition of a connective tissue graft or a collagen matrix to enhance wound stability - an experimental study in dogs

2016 ◽  
Vol 43 (4) ◽  
pp. 366-373 ◽  
Author(s):  
Rino Burkhardt ◽  
Vanessa Ruiz Magaz ◽  
Christoph H.F. Hämmerle ◽  
Niklaus P. Lang ◽  
Keyword(s):  
Experimental Study ◽  
Connective Tissue ◽  
Collagen Matrix ◽  
Connective Tissue Graft ◽  
Tissue Graft

scholarly journals Association Between Mast Cells and Collagen Maturation in Chronic Periodontitis in Humans

2018 ◽  
Vol 66 (6) ◽  
pp. 467-475 ◽  
Author(s):  
Lívia S.F. e Ribeiro ◽  
Jean N. dos Santos ◽  
Clarissa A.G. Rocha ◽  
Patricia R. Cury
Keyword(s):  
Mast Cells ◽  
Connective Tissue ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Positive Association ◽  
Collagen Fibers ◽  
Gingival Tissue ◽  
Chi Square ◽  
Probing Depth ◽  
The Relationship

Mast cells (MCs) can influence the maturation of collagen fibers. This study evaluated the relationship between the distribution and degranulation of MCs and collagen maturation in human gingival tissue in chronic periodontitis. A total of 16 specimens of patients clinically diagnosed as periodontitis and 18 controls clinically diagnosed as healthy or gingivitis were included. Immunohistochemistry and Picrosirius staining were performed to identify MCs and assess collagen fibers, respectively. Chi-square, t test, and Pearson’s correlation test ( p<0.05) were used. In control specimens, there was a positive association between MCs in the connective tissue and the presence of immature collagen ( p=0.001); in periodontitis samples, this association was not confirmed ( p≥0.12). There was no significant relationship between periodontal diagnosis and collagen maturation or MC degranulation ( p≥0.35). MC density was significantly higher ( p=0.04) in periodontitis tissue (339.01 ± 188.94 MCs/mm2) than in control tissue (211.14 ± 131.13 MCs/mm2) in the area of connective tissue containing inflammatory infiltrate. There was a correlation between the number of MCs and probing depth ( r = 0.34, p=0.04). MCs are involved in the pathogenesis of periodontal diseases and might be associated with collagen maturation in periodontal tissue during the early stages of periodontal disease pathogenesis.

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