The role of carbohydrate-protein biopolymers and hyaluronic acid at the resistance formation of hard dental tissues against pathological processes of carious and non-carious genesis

10.12737/7378 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 0-0
Author(s):  
Лещева ◽  
E. Leshcheva ◽  
Ипполитов ◽  
I. Ippolitov ◽  
Ипполитов ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Soft Tissues ◽  
Human Tooth ◽  
Cationic Protein ◽  
Thin Sections ◽  
Tooth Structure ◽  
Dental Tissues ◽  
Protein Nature ◽  
Hard Dental Tissues ◽  
Special Studies

At the series of histochemical studies hypothesis of existence of a "cationic protein" in human tooth structure and set of its amino acids components and hyaluronic acid has been checked. Taking into account histogenesis and cytogeny of tooth structure and the earlier results received from the epidermis and multilayer partly keratinous epithelium of the oral mucosa, special studies had been created, based on the original equipment preparation of paraffin sections, and thin sections of the whole teeth and modified histochemical identification technology of carbohydrate-protein biopolymers in hard dental tissues. Histological slides are prepared from extracted for orthodontic indications teeth. Sectioned and examined thin sections of teeth has investigated in transmitted light for issues related to topochemistry of carbohydrate-protein biopolymers and hyaluronic acid concerning the distribution in structures of enamel, dentin, predentin, cellular and acellular cement. Identified carbohydrate-protein biopolymers and substances of protein nature objectively fill inter prismatic enamel areas, dentin tubules and their walls, localized in the cement structure of tooth and its soft tissues forming a physiologi-cal barrier to microorganisms and their metabolic products as in the demineralizing processes as for the mechan-ical interventions during the vital activity and medical interventions.

scholarly journals CLINICAL CASES INDICATING THE ADVANTAGES OF RESTORATIONS BY CERAMIC DEPOSITS OVER COMPOSITE SEALS

2019 ◽  
Vol 15 (1) ◽  
pp. 104-108
Author(s):  
Виктория Иванова ◽  
Viktoria Ivanova ◽  
Екатерина Макарова ◽  
Ekaterina Makarova ◽  
Максим Никоноров ◽  
...  
Keyword(s):  
Modern Technology ◽  
Practical Significance ◽  
Modern World ◽  
Tooth Structure ◽  
Dental Tissues ◽  
Ceramic Inlays ◽  
All Ceramic ◽  
Hard Dental Tissues ◽  
High Level ◽  
Clinical Cases

Background. In prosthetic dentistry, the replacement of a damaged tooth structure with ceramics has become increasingly common today. The possibility of achieving a high level of aesthetics, durability and cost-effectiveness in the restoration of the coronal part of the teeth with all-ceramic inlays by the method of indirect restoration is one of the topical issues for dentists. Objectives. This article gives an idea of the possibilities of modern technology in orthopedic dentistry. The aim of the work is to identify the quality of the restoration result as compared to fillings in cavities with a degree of destruction according to TOSFI (Tooth Occlusal Surface Failure Index offered by V.Y. Milikevich) -0.3- 0.6 in the teeth of the chewing group. Methods. The article deals with the clinical cases of the replacement of insolvent fillings made of a light-cured composite with cast ceramic microprostheses. For the restoration of the molars, 2 all-ceramic inlays were made on a 5-axis open-type milling system Wieland dental zenotec select hybrid. Results. Based on dental microscopic examination and a comparative analysis of the properties and behavior of the composite fillings and ceramic inlays, one can be sure that the restoration with ceramics is much superior to the composite filling, as it represents a more reliable, durable and aesthetic option for restoring the destroyed tissues of the crowns of chewing teeth. Conclusions. In the modern world, various technologies are being created for prosthetics of hard dental tissues that could create more and more perfect restorations. Looking at the investigated examples of clinical cases and relying on the statements of many authors of works on orthopedic dentistry, we can conclude that tabs are the best alternative to fillings. The practical significance of the work consists in identifying the greater efficacy of treatment for people with significant dental defects due to microprosthetics with ceramic inserts as compared with classical treatment with composite materials.

Green Pigmentation in Human Teeth. A Stereomicroscopic Study

2014 ◽  
Vol 38 (4) ◽  
pp. 355-361 ◽  
Author(s):  
A Rakauskaite ◽  
G Juodzbalys ◽  
DH Pauza ◽  
M Cicciù
Keyword(s):  
Current Knowledge ◽  
Current Status ◽  
Primary Tooth ◽  
Human Teeth ◽  
Thin Sections ◽  
Dental Tissues ◽  
Microscopic Features ◽  
Proper Diagnosis ◽  
Uncommon Condition ◽  
Hard Dental Tissues

Green pigmentation in teeth is an uncommon condition associated with bilirubin deposits in hard dental tissues. Its occurrence can cause anxiety to both the child and parents and is not diagnosed easily by clinicians. The aim of this study is to analyze the current knowledge about the etiology, the intraoral alterations, and the macroscopic and microscopic features of green teeth pigmentation related to a high bilirubin levels. A primary tooth was extracted and manually sliced into 600 microns thin sections. The slenderized slices were examined with a light microscope AxioImager M1 to evaluate the microscopic teeth structure. The clinical characteristics of teeth may help in the diagnosis of current or past systemic diseases. Pediatricians should be able to quickly note the signs in order to perform the proper diagnosis. This study may help clinicians gain more knowledge about the current status of this uncommon pathology.

scholarly journals Forensic Dentistry – the key to the truth

2017 ◽  
Vol 64 (3) ◽  
pp. 113-120
Author(s):  
Vancho Spirov ◽  
Oliver Dimitrovski ◽  
Žaklina Menceva ◽  
Aleksej Duma ◽  
Zlatko Jakjovski
Keyword(s):  
Dna Sequences ◽  
Magnetic Particles ◽  
Soft Tissues ◽  
Dna Isolation ◽  
Human Organism ◽  
Common Source ◽  
Dna Molecules ◽  
Pulp Tissue ◽  
Dental Tissues ◽  
Hard Dental Tissues

Summary Introduction Human organism can be identified through testing and analysis of DNA sequences. The most common source of DNA for analysis is blood, soft tissues, hair, bones and teeth. Teeth represent a tissue of choice for analysis in those cases where there is high degree of degradation of other tissues. Hard tooth structure provides protection and preservation of DNA molecules. The aim was to investigate which group of teeth and dental tissue (pulp or hard dental tissues) has the greatest amount of DNA. Material and method Forty-five extracted teeth were analyzed. In the first examination 30 teeth were divided into the three groups (10 teeth each): first group were incisors, second premolars and third molars. The teeth were measured before and after the procedure of DNA isolation using special scale with precision of 0.02-0.000005ng. The procedure included grinding teeth in a blender and DNA isolation using commercial kits (isolation with magnetic particles). For the second test 15 teeth divided into two groups were used. In the first group isolation of DNA molecules was performed from pulp tissue, and in the second group from hard dental tissues. The quantification of samples was done with Quantifiler® Duo DNA Quantification Kit by Applied Biosystems. Results The greatest amount of DNA was obtained from molars (0.230011ng/μl/g) while the smallest amount of DNA was obtained from incisors and it was 0.06437ng/μl/g. In addition, the amount of DNA isolated from pulp tissue was significantly greater than that from hard dental tissues (pulp of molars obtained quantitatively the largest amount of DNA). Conclusion Main tissue to be used for the isolation of DNA from a tooth is pulp, but in those cases when it is not present (endodontic treatment), hard dental tissues provide sufficient quantity of DNA for identification procedures. The group of teeth that provides the greatest amount of DNA is molars.

Vital Tooth Whitening Techniques. A literature Review

2020 ◽  
Vol 02 ◽  
Author(s):  
Pia Chatterjee Kirk
Keyword(s):  
Soft Tissues ◽  
Treatment Time ◽  
Tooth Bleaching ◽  
Tooth Whitening ◽  
Easy Method ◽  
Tooth Structure ◽  
Dental Esthetics ◽  
Total Treatment Time ◽  
Restorative Materials ◽  
Total Treatment

Background: Vital tooth whitening has become an integral part of esthetic dentistry and remains one of the safest and most economic options today to improve dental esthetics without removing tooth structure. The tooth whitening materials have evolved into three categories: dentist-prescribed/dispensed (in office and patient home-use), and over-thecounter purchased and applied by patients. Objective: This review outlines the latest advances in dentist prescribed vital teeth whitening techniques, effects on tooth structure, soft tissues, and dental restoratives. Areas requiring additional research are also discussed. Methods: Electronic and manual literature search was conducted for key words such as tooth bleaching, and dental bleaching techniques using PubMed/MEDLINE, followed by manual selection of the studies that included whitening procedures in vital teeth. Results: The two main whitening agents are carbamide peroxide (CP) and hydrogen peroxide (HP or H2O2) whose concentration, duration of contact, and total treatment time can alter results. In addition, factors including the type of stain, and age of patient can affect results. Although whitening agents can affect tooth structure, restorative materials, and gingival tissues, the changes are temporary or can be treated using minimally invasive techniques. Conclusion: Areas requiring further research include the actual mechanism of whitening, its effect on tooth structure and restorative materials, and the development of an easy method to quantitate the degree of whitening in the dental office.

scholarly journals Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2558
Author(s):  
Mihaela Olaru ◽  
Liliana Sachelarie ◽  
Gabriela Calin
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Present Review ◽  
Modern Concept ◽  
Dental Tissues ◽  
Tissue Engineering Approach ◽  
Hard Dental Tissues ◽  
Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

The effectiveness of early local administration of hyaluronic acid in experimental traumatic ischemia of the limb muscles

2021 ◽  
Author(s):  
A.V. Shulepov ◽  
I.A. Shperling ◽  
Yu.V. Yurkevich ◽  
N.V. Shperling
Keyword(s):  
Oxygen Consumption ◽  
Hyaluronic Acid ◽  
Oxygen Saturation ◽  
Wistar Rats ◽  
Soft Tissues ◽  
Local Administration ◽  
Compression Injury ◽  
Doppler Flowmetry ◽  
Muscle Ischemia ◽  
Male Wistar Rats

The experiments were performed on 280 male Wistar rats weighing 300-340 g. The design of the study included: modeling of compression injury of the soft tissues of the thigh, local injection of the preparation of hyaluronic acid "Gialift 3.5" into the area of damage 3 hours after the cessation of compression, taking into account the results after 7, 14 and 28 days. The introduction of hyaluronic acid into the area of compression injury of soft tissues reduces the severity of myoglobinemia, increases the immunohistochemical density of VEGF-positive cells, improves the perfusion characteristics of microcirculation, oxygen saturation, and specific oxygen consumption by tissues. Keywords: traumatic muscle ischemia, hyaluronic acid, myoglobin, microcirculation, metabolism, laser Doppler flowmetry, morphometry.

scholarly journals Soft Tissue Applications of Er,Cr:YSGG Laser in Pediatric Dentistry

2017 ◽  
Vol 10 (2) ◽  
pp. 188-192 ◽  
Author(s):  
Ferah Rehman ◽  
Vivek Chaturvedy
Keyword(s):  
Soft Tissue ◽  
Laser Energy ◽  
Case Series ◽  
Pediatric Dentistry ◽  
Patient Comfort ◽  
Operating Efficiency ◽  
Hard Tissue ◽  
Contact Mode ◽  
Tooth Structure ◽  
Dental Tissues

ABSTRACT Soft tissue laser, such as diode and Nd:YAG lasers were initially used in soft tissue lesions because of its increased success rate. It was because of the fact that these lasers were well-absorbed by chromophores, such as hemoglobin and melanin which are found abundant in the oral mucosa. The introduction of erbium family in 1990 comprising the Er:YAG and Er,Cr:YSGG lasers made the hard tissue laser a boon for dentistry. Erbium, chromium-doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG) was introduced in 1997 for the surgical needs of clinical dentistry in general practice. The erbium belongs to the rare earth which is embedded in a host crystal. The actual lasing process takes place in the Er ion Er3+. Two host crystals consisting of yttrium, aluminum, and garnet (Y3A5O12) and yttrium, scandium, gallium, and garnet (Y3Sc2Ga3O12) are added to the erbium. The interest to use these hard tissue laser in the treatment of soft tissue lesion was because of the properties of these lasers which are well-absorbed by chromophore water apart from hydroxy appetite crystals. Erbium laser energy is absorbed by collagen, hydroxyapatite, and water components. It allows the laser to cut soft tissue, tooth structure, and bone. In the noncontact mode, the incision is scalpel-like, with very little hemostasis. In contact mode, it performs soft tissue sculpting with adequate hemostasis. The Er,Cr:YSGG is the world's most advanced dental laser, which is ideal all-tissue laser because all dental tissues contain water, for the multidisciplinary dentist who performs a broad spectrum of procedures. It delivers the highest level of clinician control, operating efficiency, flexibility in tip, and accessory selection. For optimal clinical results and patient comfort in hard and soft tissue procedures, the erbium lasers have set a new standard of clinical performance. The present case series aims to highlight the various soft tissue applications of Er,Cr:YSGG (Waterlase Biolase®, Biolase, Inc, San Clemente, California, USA) in pediatric patients. How to cite this article Kumar G, Rehman F, Chaturvedy V. Soft Tissue Applications of Er,Cr:YSGG Laser in Pediatric Dentistry. Int J Clin Pediatr Dent 2017;10(2):188-192.

scholarly journals Radiopacity of restorative materials using digital images

2006 ◽  
Vol 14 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Leda Maria Pescinini Salzedas ◽  
Mário Jefferson Quirino Louzada ◽  
Antonio Braz de Oliveira Filho
Keyword(s):  
Restorative Material ◽  
Radiographic Image ◽  
Equivalent Thickness ◽  
Tooth Structure ◽  
Suitable Material ◽  
Dental Tissues ◽  
Permanent Molar ◽  
Restorative Materials ◽  
Phosphor Plate ◽  
The Mean

The radiopacity of esthetic restorative materials has been established as an important requirement, improving the radiographic diagnosis. The aim of this study was to evaluate the radiopacity of six restorative materials using a direct digital image system, comparing them to the dental tissues (enamel-dentin), expressed as equivalent thickness of aluminum (millimeters of aluminum). Five specimens of each material were made. Three 2-mm thick longitudinal sections were cut from an intact extracted permanent molar tooth (including enamel and dentin). An aluminum step wedge with 9 steps was used. The samples of different materials were placed on a phosphor plate together with a tooth section, aluminum step wedge and metal code letter, and were exposed using a dental x-ray unit. Five measurements of radiographic density were obtained from each image of each item assessed (restorative material, enamel, dentin, each step of the aluminum step wedge) and the mean of these values was calculated. Radiopacity values were subsequently calculated as equivalents of aluminum thickness. Analysis of variance (ANOVA) indicated significant differences in radiopacity values among the materials (P<0.0001). The radiopacity values of the restorative materials evaluated were, in decreasing order: TPH, F2000, Synergy, Prisma Flow, Degufill, Luxat. Only Luxat had significantly lower radiopacity values than dentin. One material (Degufill) had similar radiopacity values to enamel and four (TPH, F2000, Synergy and Prisma Flow) had significantly higher radiopacity values than enamel. In conclusion, to assess the adequacy of posterior composite restorations it is important that the restorative material to be used has enough radiopacity, in order to be easily distinguished from the tooth structure in the radiographic image. Knowledge on the radiopacity of different materials helps professionals to select the most suitable material, along with other properties such as biocompatibility, adhesion and esthetic.

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