AMPA Ionotropic Glutamate Receptors Pathogenesis Symptomatic Epilepsy in Gliomas of the Cerebral Hemispheres

10.12737/5909 ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 97-100
Author(s):  
Костюкевич ◽  
A. Kostyukevich ◽  
Очколяс ◽  
V. Ochkolyas ◽  
Скоромец ◽  
...  
Keyword(s):  
Ampa Receptors ◽  
Maximum Level ◽  
Epileptic Syndrome ◽  
Symptomatic Epilepsy ◽  
Glur1 Subunit ◽  
Growth Features ◽  
Morphological Research ◽  
Nr2a Subunit

The purpose of the research is to study the role of AMPA receptors of glutamate in the mechanisms of the development of epi-lepsy in the patients with gliomas of the big hemispheres of a brain. Materials and methods: 92 patients with gliomas of the big hemispheres of a brain have been examined. Immune enzyme method of semi-quantitative determination of the level of auto-antibodies to NR2A subunit of NMDA and GluR1 subunit of AMPA receptors of glutamate was used. Results: The frequency and clinical features of symptomatic epilepsy have been studied. The reaction of NMDA and of AMPA receptors of glutamate depending on localization and degree of malignance and features of clinical course of the disease, have been examined. Conclusion: A high-technology of patho-morphological research of brain tumors made in recent years has revealed that glial tumors start releasing glutamate by themselves, as their anaplasia level increases, which causes exitotoxical effect in anatomical lines of peritumoral area. Such blastomastoma growth features form specificity of clinical aspect of disease, including epileptic syndrome. It is shown dominative increase the level of auto-antibodies to GluR1 subunit of AMPA receptors of glutamate in the patients with gliomas, current with epileptic syndrome. Under the effect of the tumor on the frontal and temporal share the maximum level of autoantibodies, mainly to GluR1 subunit of AMPA receptors of glutamate is registered.

scholarly journals Аlterationdegree of NMDA and AMPA receptors of glutamate in the pathogenesis of the disease and technological peculiarities of surgical treatment of the hemispheric glioma-swith the epileptic syndrome

2014 ◽  
Vol 21 (2) ◽  
pp. 23-29
Author(s):  
V. N. Ochkolyas ◽  
M. B. Volov
Keyword(s):  
Glutamate Receptors ◽  
Ampa Receptors ◽  
Molecular Level ◽  
Radical Surgery ◽  
Total Removal ◽  
Epileptic Syndrome ◽  
Immunoenzyme Method ◽  
Glur1 Subunit ◽  
Nr2a Subunit

The development of effective approaches to the treatment of patients with symptomatic tumor epilepsy was determined by the level of our fundamental knowledge of the basic mechanisms of epileptogenesis on cellular and molecular level. 92 patients with gliomas of the cerebral hemisphereswere examined. Immunoenzyme method ofsemiquantitative determination of the level of autoantibodies to NR2A subunit of NMDA and GluR1 subunit of AMPA receptors of glutamate was used. The reaction of NMDA and AMPA glutamate receptors depending on the availability of an epileptic syndrome in the clinical picture of the disease and the extent of radical surgery has been studied. Pre-emptive increase of the level of autoantibodies to GluR1 subunit of AMPA receptors of glutamate in patients with hemispheric gliomaswith epileptic syndrome was demonstrated. It was foundonly the total removal of the tumor reduced significantly the level of autoantibodies to ionotropic glutamate receptors.

scholarly journals Epileptic syndrome in the clinical picture of the diseaseand the level of antibodies to GluR1 subunit of AMPA glutamate receptors in the patients with gliomas in the cerebral hemispheres of a brain

2013 ◽  
Vol 20 (4) ◽  
pp. 73-76
Author(s):  
V. N. Ochkolyas ◽  
A. F. Gurchin ◽  
A. A. Skoromets ◽  
A. V. Kostyukevich
Keyword(s):  
Glutamate Receptors ◽  
Ampa Receptors ◽  
Clinical Course ◽  
Cerebral Hemispheres ◽  
Maximal Level ◽  
Ampa Glutamate Receptors ◽  
Epileptic Syndrome ◽  
Immunoenzyme Method ◽  
Symptomatic Epilepsy ◽  
Glur1 Subunit

The authors examined 92 patients with gliomas in the cerebral hemispheres. The immunoenzyme method was used for semiquantitative assay of the level of autoantibodies to GluR1 subunit of AMPA receptors of glutamate. Frequency and clinical features of symptomatic epilepsy, the reaction of AMPA receptors of glutamate depending on localization and degree of malignance and features of clinical course of the disease wereinvestigated as well. Marked increase of the level of autoantibodies to GluR1 subunit of AMPA receptors of glutamate was noticed in the patients with gliomas associated with epileptic syndrome. Maximal level of autoantibodies to GluR1 subunit of AMPA receptors of glutamate seen under the effect of the tumor on the frontal and temporal lobels.

THE SIGNIFICANCE OF THE CONTENT OF LOW AND MEDIUM MOLECULAR WEIGHT SUBSTANCES AND OLIGOPEPTIDES IN THE PATHOGENESIS OF VARICELLA

2021 ◽  
pp. 185-192
Author(s):  
Margity M.M. ◽  
Marzhokhova M.Y. ◽  
Hadzegova S.B.
Keyword(s):  
Molecular Weight ◽  
Infectious Diseases ◽  
Blood Plasma ◽  
Blood Cells ◽  
Maximum Level ◽  
Body Fluids ◽  
Endogenous Intoxication ◽  
Complicated Course

Endogenous intoxication syndrome is an important link in the pathogenesis of many infectious diseases. The role of low and medium molecular weight substances and oligopeptides acting as indicators of endogenous intoxication syndrome in the pathogenesis of varicella was studied, depending on the period of the disease, the severity of the course and the presence of complications. Based on this goal, 125 patients with varicella aged 18 to 49 years were examined. Determination of the content of substances of low and medium molecular weight in biological body fluids (blood plasma, red blood cells and urine) was carried out by the method of M. Ya.Malakhova (1995) on a spectrophotometer SF-46. The Lowru (1951) method was used to determine the level of oligopeptides in body fluids. The maximum level of substances of low and medium molecular weight was observed in patients with a complicated course of varicella during the height of the disease, as well as in patients with a severe course of the disease during the height. When determining the oligopeptides, the highest values were also obtained at the height of the disease in the severe course, as well as in the complicated course at admission. During the examination, the dependence of the studied indicators on the period of the disease, the severity, as well as the presence of complications was revealed. Thus, the most pronounced changes in the level of low and medium molecular weight substances and oligopeptides were observed in severe, and the least – in the mild course of varicella in all the studied media. In patients with developed complications, the disease was more severe, while the changes in the studied parameters were more pronounced.

The Role of Quercetin in Gene Expression of GluR1 Subunit of AMPA Receptors, and NR2A and NR2B Subunits of NMDA Receptors in Kainic Acid Model of Seizure in Mice

2016 ◽  
Vol 19 (5) ◽  
Author(s):  
Sahar Moghbelinejad ◽  
Ghazaleh Mohammadi ◽  
Fatemeh Khodabandehloo ◽  
Reza Najafipour ◽  
Taghi Naserpour ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nmda Receptors ◽  
Kainic Acid ◽  
Ampa Receptors ◽  
Glur1 Subunit

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

On The Mechanism Of Heparin-Induced Potentiation Of Platelet Aggregation

1981 ◽  
Author(s):  
M Yamamoto ◽  
K Watanabe ◽  
Y Ando ◽  
H Iri ◽  
N Fujiyama ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Platelet Activation ◽  
Coagulation Factors ◽  
Marked Enhancement ◽  
Matrix Bound ◽  
Induced Aggregation ◽  
Aggregation Of Platelets ◽  
Plasma Heparin

It has been suggested that heparin caused potentiation of aggregation induced by ADP or epinephrine. The exact mechanism of heparin-induced platelet activation, however, remained unknown. In this paper, we have investigated the role of anti-thrombin III ( AT ) in heparin-induced platelet activation using purified AT and AT depleted plasma. When ADP or epinephrine was added to citrated PRP one minute after addition of heparin ( 1 u/ml, porcine intestinal mucosal heparin, Sigma Co. USA ), marked enhancement of platelet aggregation was observed, compared with the degree of aggregation in the absence of heparin. However, in platelet suspensions prepared in modified Tyrode’s solution, heparin exhibited no potentiating effect on platelet aggregation induced by epinephrine or ADP. Potentiation of epinephrine- or ADP-induced platelet aggregation by heparin was demonstrated when purified AT was added to platelet suspensions at a concentration of 20 μg/ml. AT depleted plasma, which was prepared by immunosorption using matrix-bound antibodies to AT, retained no AT, while determination of α1-antitrypsinα2- macroglobulin and fibrinogen in AT depleted plasma produced values which corresponded to those of the original plasma when dilution factor was taken into account. The activities of coagulation factors were also comparable to those of the original plasma. Heparin exhibited potentiating effect on ADP- or epinephrine-induced aggregation of platelets in original plasma, but no effect in AT depleted plasma. When purified AT was added back to AT depleted plasma at a concentration of 20 μg/ml, potentiation of platelet aggregation by heparin was clearly demonstrated.Our results suggest that effect of heparin on platelet aggregation is also mediated by anti-thrombin III.

ІНТЕРПРЕТАЦІЯ КАТЕГОРІЇ РОЗВИТКУ В СУЧАСНІЙ ПСИХОЛОГІЇ

1970 ◽  
Vol 8 (1) ◽  
pp. 22-30
Author(s):  
Анжеліка Шамне
Keyword(s):  
Cultural Context ◽  
Developmental Psychology ◽  
Structure And Dynamics ◽  
Modern Psychology ◽  
Development Site ◽  
Approaches To Study ◽  
Methodological Approaches ◽  
Self Motion

У статті розглянуто сучасні підходи до інтерпретації категорії розвитку, розкрито теоретичні  та методологічні підходи до вивчення категорії розвитку у сучасній психології, визначено її психологічний  зміст,   моделі,   структуру   та   динаміку.   Категорія   розвитку   розглядається   як   епіцентр   наукової  проблематики у психології та як поняття інтегративного типу. Розвиток проаналізовано як категорію,  явище і проблему психології розвитку в різних аспектах аналізу. Розглянуто місце розвитку в системі  споріднених психологічних понять. У статті також аналізуються психологічні аспекти теоретичних та  методологічних  постнекласичних  тенденцій  вивчення  природи,  характеру  та  визначення  психічного  розвитку. Постнекласична парадигма та плюралістична методологія пізнання визначають розмитість  дисциплінарної мови  та  врахування  ролі  соціокультурного  контексту  при  вивченні  психологічних явищ.  Важливими тенденціями сучасного теоретико-методологічного стану психологічних досліджень розвитку  також є визнання неефективності моністичного підходу до його вивчення, взаємозв'язок теоретичних ідей  та   спроби   створення   метатеоретичних   схем,   постнекласичне   розуміння   розвитку   як   принципово  незавершеного   процесу   саморуху,   актуалізація   антропологічного   діапазону   проблем   та   посилення  спрямованості на роль культурного контексту в дослідженні розвитку людини.  The article deals with the modern approaches to the interpretation of the category of development, reveals  the theoretical and methodological approaches to study of development in modern psychology, its psychological  content, patterns, structure and dynamics. Category of development is viewed as an epicenter of scientific issues in  modern  psychology  and  the  concept  of  the  integrative  type.  Category  of  development  is  considered  as  the  phenomenon  and  the  problem  of  developmental  psychology  in  various  aspects  of  the  analysis.  Analyzed  the  development site in the related psychological concepts. The article analyzes the psychological aspects of theoretical  and methodological postnonclassical contemporary trends in the study of nature, character, and determination of  mental  development.  Postnonclassical  paradigm  and  pluralistic  methodology  of  knowledge  determine  the  disciplinary blurring and increase of the role of the analysis of socio-cultural context in the study of psychological  phenomenon. The important tendencies of modern theoretical and methodological state of psychological researches  of development are facts of inefficiency of the monistic approach to its study, interconnection of theoretical ideas  and   attempts   of  creating   metatheoretical   schemes,   postnonclassical   understanding   of   development   as   a  fundamentally  uncompleted  process  of  self-motion,  actualization  of  anthropological  range  of  problems  and  strengthening of focus on the role of cultural context in research of human development.   

Role of the family focused technologies in the clinical course of pregnancy at women of high obstetric risk

2016 ◽  
pp. 64-66
Author(s):  
S.Yu. Vdovichenko ◽  
Keyword(s):  
Comparison Group ◽  
Traditional Approach ◽  
Married Couple ◽  
Premature Births ◽  
Individual Support ◽  
The Family ◽  
Risk Patients ◽  
Individual Preparation

The objective: to show a role of the family focused technologies in depression of frequency of pathology of pregnancy at women of high obstetric risk. Patients and methods. For determination of efficiency of prophylaxis of pathology of pregnancy on the basis of use of the family focused technologies complex clinical-psychological and laboratory and tool examination of 300 women with factors of obstetric risk which were divided into two groups was conducted. In the main group – 182 women with motivation on partner labors to which provided training on system of individual preparation of married couple to labors. The comparison group consisted of 118 women who were not in prenatal training and had individual support in childbirth, with the traditional approach to pain management. Results. Use of the family focused technologies during pregnancy allows to reduce significantly the frequency of the main complications of pregnancy, especially not incubation and premature births. Conclusion. In our opinion, the technique is simple, available and can widely be used in practical health care at women with high obstetric risk. Key words: obstetric risk, the family focused technologies, prophylaxis.

PERANAN INSPEKTORAT KABUPATENSUMENEP DALAM MENCEGAH DAN MENDETEKSI FRAUD DI DINAS PENDIDIKAN SUMENEP

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Astri Furqani ◽  
Hafidhah .
Keyword(s):  
Local Government ◽  
Qualitative Study ◽  
Asset Management ◽  
Sampling Technique ◽  
Research Data ◽  
Qualitative Approach ◽  
Primary Data ◽  
The Government

In this era, a lot of activities that can not be separated from the practice of cheating or fraud , no exception In the government . Inspektorat Sumenep is the leading institution of internal Local Government in preventing and detecting fraud in the Local Government appropiate Perbup Sumenep No. 29 of 2008 . Dinas Pendidikan Sumenep need attention on the issue . This is due to Dinas Pendidikan Sumenep an agency with the largest number of assets and managing large budgets . These conditions led to the formulation of the problem is How Inspektorat Sumenep role in preventing and detecting fraud in Dinas Pendidikan Sumenep. This study used a qualitative approach in which the focus of this study is Inspektorat role in preventing and detecting fraud at Dinas Pendidikan Sumenep . Primary data obtained by direct interviews with the parties directly related to the determination of the source of research data in a qualitative study using nonprobability sampling . The sampling technique used was purposive sampling . The conclusion of this study, role of the Inspektorat Sumenep in the prevention of fraud in Dinas Pendidikan Sumenep still not maximal . This is due to Inspektorat Sumenep not supervise from the planning / budgeting and not optimal in overseeing and assisting the implementation of the SPIP as an instrument of fraud prevention in Dinas Pendidikan Sumenep . The role of Inspektorat Sumenep in the detection of fraud in Dinas Pendidikan Sumenep done by conducting an audit of financial and asset management in each financial year.Keywords: fraud, government, inspektorat.

Sign in / Sign up

Export Citation Format

Share Document