Plasticity of the Muscles in the Protein Sensitization. Involvement of Cholinergic asnd Purinergic Mechanisms

10.12737/3300 ◽  
2014 ◽  
Vol 21 (1) ◽  
pp. 6-12
Author(s):  
Теплов ◽  
O. Teplov ◽  
Миннебаев ◽  
M. Minnebaev ◽  
Фархутдинов ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Extensor Digitorum Longus ◽  
Striated Muscle ◽  
Contractile Function ◽  
Experimental Models ◽  
Extensor Digitorum ◽  
Chronic Obstructive ◽  
Respiratory Muscle Fatigue ◽  
Development Of Resistance ◽  
Protein Sensitization

The authors investigated the involvement of the adenosine triphosphate (ATF) in the mechanisms of plasticity in the striated muscle in condition of protein sensitization. Contractile function and non-quantum secretion of the acetylcholine in the endplate zone were studied in isolated skeletal muscles of mouse legs (musculus soleus and musculus extensor digitorum longus) and strips musculus diaphragm. The authors studied the soleus and diaphragm dynamics of the force vector of muscle contraction after effects of exogenous adenosine triphosphate correlated with changes non-quantum secretion of acetylcholine in all the experimental models. However, the extent of these changes in sensitized animals is less pronounced than in the controls. It is suggested that adenosine is a party change mechanisms and functional properties soleus and diaphragm at the protein sensitization. The basis of the development of resistance, stability to long as the external stress, and at the initial stages of the experimental allergic these muscles are dependent adenosine mechanisms regulating their sensitivity to acetylcholine. These processes provide a reduction in respiratory muscle fatigue in hypoxia that occurs in asthma, chronic obstructive pulmonary disease and broncho-spastic syndrome, as well in increase their performance during prolonged physical activity. The reasons for changes in the force reduction extensor digitorum longus at a protein sensitization doesn’t associated with the mechanisms of muscle excitation mediated by adenosine triphosphate.

scholarly journals Possible involvement of adenosine triphosphate in the mechanisms of protein sensibilization effect on the functional properties of the diaphragm and skeletal muscles

2012 ◽  
Vol 93 (1) ◽  
pp. 113-116
Author(s):  
A Yu Teplov ◽  
A M Farkhutdinov ◽  
S N Grishin ◽  
M M Minnebaev ◽  
V I Torshin
Keyword(s):  
Adenosine Triphosphate ◽  
Functional Properties ◽  
Skeletal Muscles ◽  
Contractile Function ◽  
Muscular Contraction ◽  
Postsynaptic Membrane ◽  
Experimental Models ◽  
Force Vector ◽  
Leg Muscles ◽  
Before And After

Aim. To study the effect of adenosine triphosphate on the contractile function and non-quantum secretion of acetylcholine at the endplate zone (H-effect) of isolated mouse muscles on the background of protein sensibilization. Methods. The experiments were performed on white mice. Sensibilization was carried out by ovalbumin with an aluminum hydroxide gel. Mechanomyography studies were performed on isolated preparations of the diaphragm and of two leg muscles in isometric conditions. The contractions were recorded by a photoelectric converter. In order to study the condition of the postsynaptic membrane of the muscle fibers measured was the non-quantum secretion of acetylcholine. Compared were the parameters of muscle contraction before and after 5 min of perfusion with a solution of adenosine triphosphate. Results. In the diaphragm and in the soleus muscle the dynamics of the force vector of the muscular contraction correlated with the changes in the H-effect in all the studied experimental models. However, the extent of these changes in the sensibilized animals is less pronounced. Conclusion. It is possible that adenosine triphosphate affects the functional properties of both muscle during protein sensibilization; the change in the contraction force of the long extensor digitorum muscle during sensibilization is not related to the mechanisms of muscle excitation, mediated by adenosine triphosphate.

Simultaneous Assessment of Isometric Forces in Fast- and Slow-Twitch Muscles of Single Rat Hindlimbs in Situ

1996 ◽  
Vol 21 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Andrew J. Carvalho ◽  
Nancy H. McKee
Keyword(s):  
Extensor Digitorum Longus ◽  
Mechanical Performance ◽  
Contractile Function ◽  
Small Strain ◽  
Extensor Digitorum ◽  
Published Data ◽  
Slow Twitch ◽  
Isometric Twitch ◽  
Simultaneous Assessment

An apparatus, consisting of a pair of small strain gauge transducers, was designed for the simultaneous assessment of isometric contractile function in two muscles, composed of a predominance of either fast- or slow-twitch fibers, and within a single rat hindlimb in situ. This facilitates assessment of mechanical performance of two separate muscles under identical conditions. In anesthetized rats (N = 10), the voltages and frequencies required to produce isometric twitch and tetanic forces from the soleus (SOL) and extensor digitorum longus (EDL) muscles were determined. The apparatus was then used to demonstrate the simultaneous assessment of forces produced by the SOL and EDL from the same hindlimbs (n = 5) during 30 min of fatigue and 30 min of recovery. With this apparatus, data collected were comparable to published data. The apparatus can be used for the simultaneous assessment of isometric contractile function and fatigue in both a fast- and a slow-twitch muscle of a single rat hindlimb in situ. Key words: model, fatigue, ischemia, soleus, extensor digitorum longus

scholarly journals The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study

2007 ◽  
Vol 21 (3) ◽  
pp. 151-160 ◽  
Author(s):  
Ozlem Bozkurt ◽  
Mehmet Dincer Bilgin ◽  
Feride Severcan
Keyword(s):  
Diabetes Mellitus ◽  
Extensor Digitorum Longus ◽  
Structural Changes ◽  
Experimental Models ◽  
Extensor Digitorum ◽  
Nucleic Acid Content ◽  
Type I ◽  
Muscle Tissues ◽  
Chronic Disorder ◽  
Edl Muscle

Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in medical research. Type I DM was induced in rats by injection of streptozotocin (STZ) which is one of the most popular experimental models. In diabetes, a significant increase was observed in lipid order together with an increase in hydration of phospholipid molecules in membrane structure. There was a decrease in lipid and nucleic acid content in diabetic EDL muscles. A dramatic increase in the bandwidth of amide II band (1540 cm−1) and shifting of the position of this band to lower frequency values in diabetes was observed indicating structural changes occurring in proteins of diabetic EDL muscles.

Profiles of connectin (titin) in atrophied soleus muscle induced by unloading of rats

2003 ◽  
Vol 94 (3) ◽  
pp. 897-902 ◽  
Author(s):  
Katsumasa Goto ◽  
Ryoko Okuyama ◽  
Masanori Honda ◽  
Hiroshi Uchida ◽  
Tatsuo Akema ◽  
...  
Keyword(s):  
Extensor Digitorum Longus ◽  
Contractile Function ◽  
Muscle Fibers ◽  
Extensor Digitorum ◽  
Hindlimb Suspension ◽  
Molecular Weights ◽  
Band Region ◽  
Wet Weight ◽  
Fast Twitch ◽  
Significant Increment

Responses of the properties of connectin molecules in the slow-twitch soleus (Sol) and fast-twitch extensor digitorum longus muscles of rats to 3 days of unloading with or without 3-day reloading were investigated. The wet weight (relative to body wt) of Sol, not of extensor digitorum longus, in the unloaded group was significantly less than in the age-matched control ( P < 0.05). Immunoelectron microscopic analyses showed that a monoclonal antibody against connectin (SM1) bound to the I-band region close to the edge of the A band at resting length and moved reversibly away from the Z line as the muscle fibers were stretched. In Sol, the displacement of the SM1-bound dense spots in response to stretching decreased after hindlimb suspension. There were no changes in the molecular weights and the percent distributions of α- and β-connectin in both muscles after hindlimb suspension. A significant increment of percent β-connectin in Sol was observed after 3 days of reloading after hindlimb suspension ( P < 0.05). It is suggested that the elasticity of connectin filaments in the I-band region of the atrophied Sol fibers was reduced relative to that of the control fibers. The lack of the elasticity in atrophied muscle fibers may cause a decrease in contractile function.

scholarly journals Effects of glucose on contractile function, [Ca2+]i, and glycogen in isolated mouse skeletal muscle

2002 ◽  
Vol 282 (6) ◽  
pp. C1306-C1312 ◽  
Author(s):  
Ingrid Helander ◽  
Håkan Westerblad ◽  
Abram Katz
Keyword(s):  
Skeletal Muscle ◽  
Extensor Digitorum Longus ◽  
Contractile Function ◽  
Extensor Digitorum ◽  
Fluorescence Ratio ◽  
Tetanic Force ◽  
Mouse Skeletal Muscle ◽  
Control Value ◽  
Extracellular Glucose ◽  
Initial Force

Extensor digitorum longus muscles were stimulated to contract to fatigue and allowed to recover for 2 h in the absence or presence of 5.5 or 11 mM extracellular glucose. This was followed by a second fatigue run, which ended when the absolute force was the same as at the end of the first run. During the first fatigue run, the fluorescence ratio for indo 1 increased [reflecting an increase in myoplasmic free Ca2+ concentration ([Ca2+]i)] during the initial tetani, peaking at ∼115% of the first tetanic value, followed by a continuous decrease to ∼90% at fatigue. During the first fatigue run, myofibrillar Ca2+ sensitivity was significantly decreased. During the second run, the number of tetani was 57 ± 6% of initial force in muscles that recovered in the absence of glucose and 110 ± 6 and 119 ± 2% of initial force in muscles that recovered in 5.5 and 11 mM glucose, respectively. Fluorescence ratios during the first, peak, and last tetani did not differ significantly between the first and second fatigue runs during any of the three conditions. Glycogen decreased by almost 50% during the first fatigue run and did not change further after recovery in the absence of glucose. After recovery in the presence of 5.5 and 11 mM glucose, glycogen increased 32 and 42% above the nonstimulated control value ( P < 0.01). These data demonstrate that extracellular glucose delays the decrease of tetanic force and [Ca2+]i during fatiguing stimulation and that glycogen supercompensation following contraction can occur in the absence of insulin.

CONDITION OF HEMODYNAMICS IN THE PULMONARY CIRCULATION OF PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE (COPD) CONCURRENT WITH METABOLIC SYNDROME WITH HYPERTROPHY AND ATROPHY OF THE MYOCARDIUM

2019 ◽  
Vol 72 (8) ◽  
pp. 1491-1493
Author(s):  
Viktor P. Boriak ◽  
Svitlana V. Shut’ ◽  
Tetiana A. Trybrat ◽  
Olena V. Filatova
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Metabolic Syndrome ◽  
Right Ventricular ◽  
Pulmonary Disease ◽  
Pulmonary Circulation ◽  
Contractile Function ◽  
Ventricular Myocardium ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
The Right

Introduction: In recent years, COPD is observed as not an isolated, but an associated pathology, in particular, concurrent with metabolic syndrome. The aim of the research is to identify the differences in changes of the rheopulmonography parameters (RPG) depending on the presence of hypertrophy or atrophy of the right ventricular myocardium in patients with COPD concurrent with metabolic syndrome.. Materials and methods: We studied changes in rheopulmonography (RPG) in 145 patients with chronic obstructive pulmonary disease (COPD) concurrent with metabolic syndrome. Results: We detected precapillary hypertension of the pulmonary circulation in patients with right ventricular myocardial hypertrophy: anacrotism serration; flattened peak of the systolic wave; decreased Vcp; high placement of incisura; horizontal course of catacrotism; decreased amplitude of the systolic wave (in this case, due to a greater increase in the resistance of the blood flow in the pulmonary vessels than the decreased impact volume of the right ventricle); prolonged Q-a (in this group of patients, it depends more on hypertension of the pulmonary circulation than on the reduction of contractile function of the myocardium). In atrophy of the right ventricular myocardium, the following changes in the RPG were revealed: decreased systolic wave at its dramatic rise; prolonged Q-a (in this case, due to the weakened heart contraction); Vmax reduction (it reflects the reduction of myocardial contractility); in hypertrophy of the myocardium, Vcp., unlike RPG, does not decrease, which is explained by the decrease in the pressure of the pulmonary circulation. Conclusions: We believe that these changes in RPG allow differentiating hypertrophy and right ventricular myocardial atrophy along with established diagnostic criteria, and can be used as markers for the diagnosis and treatment of COPD concurrent with metabolic syndrome.

Human growth hormone treatment of hypophysectomized rats increases the proportion of type-1 fibres in skeletal muscle

1989 ◽  
Vol 123 (3) ◽  
pp. 429-NP ◽  
Author(s):  
C. M. Ayling ◽  
B. H. Moreland ◽  
J. M. Zanelli ◽  
D. Schulster
Keyword(s):  
Skeletal Muscle ◽  
Fibre Type ◽  
Extensor Digitorum Longus ◽  
Human Growth ◽  
Hormone Treatment ◽  
Pituitary Hormones ◽  
Extensor Digitorum ◽  
Replacement Treatment ◽  
Hypophysectomized Rats

ABSTRACT The studies describe alterations after hypophysectomy in the proportion of the type-1 and type-2 fibres in rat skeletal muscles, and the effects of replacement treatment with pituitary human (h) GH. Cytochemical analysis of myosin ATPase, succinate dehydrogenase and lactate dehydrogenase activities in sections of rat hind limb muscles were used as markers of fibre type and revealed that hypophysectomy reduced the proportion of type-1 fibres by 50% in soleus and in extensor digitorum longus muscles. This reduction in the proportion of type-1 fibres was accompanied by the appearance of transitional fibres (type 2C/1B). Following seven daily injections of hGH (60 mIU/day) to hypophysectomized rats, the proportion of type-1 fibres in both soleus and in extensor digitorum longus was increased with a concomitant reduction in the number of transitional fibres. After 11 days of treatment, all these transitional fibres had reverted back to type-1 fibres. Only hGH was observed to elicit this effect; injections of other pituitary hormones had no effect on the proportions of these transitional fibres. These alterations in fibre type occurred more rapidly than the changes reported after prolonged electrical stimulation of muscle or following extended exercise. These findings suggest that hypophysectomy and GH injection can result in a rapid alteration in the fibre composition of skeletal muscle, which may have important implications in terms of the resistance to fatigue and speed of contraction of the muscle. Journal of Endocrinology (1989) 123, 429–435

scholarly journals Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis

1971 ◽  
Vol 121 (5) ◽  
pp. 817-827 ◽  
Author(s):  
R. C. Hider ◽  
E. B. Fern ◽  
D. R. London
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Incubation Medium ◽  
Extensor Digitorum Longus ◽  
Extensor Digitorum ◽  
Longus Muscle ◽  
Kinetics Of

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

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