Investigation of Genotoxicity and Cytotoxicity of Nanocomposite Highly Dispersed Silica with Silver Nanoparticles

10.12737/2725 ◽  
2013 ◽  
Vol 20 (4) ◽  
pp. 44-47
Author(s):  
Савченко ◽  
D. Savchenko
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Agents ◽  
High Efficiency ◽  
Practical Interest ◽  
Eukaryotic Cell ◽  
New Drugs ◽  
Carcinoma Larynx ◽  
Highly Dispersed Silica ◽  
Toxicological Studies ◽  
Highly Dispersed

Provision of high efficiency and quality of drugs developed on the basis of nanotechnology is possible only under the requirements for the development, research, production and introduction of new pharmacological agents. Determination of genotoxicity and cytotoxicity is the first stage of toxicological studies of newly synthetized substances, the aim of which is to determine the ability to induce primary DNA and cell cultures damages. Such studies provide information about primary toxic effects of substances and constitute a basis for confirming the safety of new drugs. Considering a constant need for the introduction of new antimicrobial agents for treatment of intestinal infections, it is expedient to develop combinative drugs which will have better efficacy and safety. Significant scientific and practical interest has study of drugs based on nanosilver, which is active against antibiotic-resistant microorganisms. By the Department of Pharmacology and Clinical Pharmacology of Bogomoletz National Medical University and Chuiko Institute of Surface Chemistry NAS of Ukraine was specifically designed and synthesized nanocomposite highly dispersed silica with silver nanoparticles, which is considered as a promising drug with a complex of antibacterial and sorption-detoxication properties. The purpose of this work is to study the cytotoxicity and genotoxicity of nanocomposite highly dispersed silica with silver nanoparticles. The degree of DNA damage by nanocomposite was studied by alkaline gel electrophoresis of isolated cell test culture CHO-Kl. Investigation of cytotoxicity was carried out on passaged eukaryotic cell culture of human epidermoid carcinoma larynx (Hep-2). It is shown that nanocomposite highly dispersed silica with silver nanoparticles does not have genotoxic properties, and its cytotoxicity disappears at concentrations below 0,007%.

scholarly journals Endophytic Bacteria Enterobacter hormaechei Fabricated Silver Nanoparticles and Their Antimicrobial Activity

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 511
Author(s):  
Tahmina Monowar ◽  
Md. Sayedur Rahman ◽  
Subhash J. Bhore ◽  
Kathiresan V. Sathasivam
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
New Drugs ◽  
Biological Synthesis ◽  
Green Approach ◽  
Vis Spectroscopy ◽  
Enterobacter Hormaechei

Antimicrobial resistance (AMR), one of the greatest issues for humankind, draws special attention to the scientists formulating new drugs to prevent it. Great emphasis on the biological synthesis of silver nanoparticles (AgNPs) for utilization in single or combinatorial therapy will open up new avenues to the discovery of new antimicrobial drugs. The purpose of this study was to synthesize AgNPs following a green approach by using an endophytic bacterial strain, Enterobacter hormaechei, and to assess their antimicrobial potential against five pathogenic and four multidrug-resistant (MDR) microbes. UV-Vis spectroscopy, fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and zeta potential (ζ) were used to characterize the synthesized AgNPs. Endophytic E. hormaechei-mediated AgNPs (Eh-AgNPs) were represented by a strong UV-Vis absorbance peak at 418 nm within 5 min, forming spherical and polydispersed nanoparticles in the size range of 9.91 nm to 92.54 nm. The Eh-AgNPs were moderately stable with a mean ζ value of −19.73 ± 3.94 mV. The presence of amine, amide, and hydroxyl functional groups was observed from FTIR analysis. In comparison to conventional antibiotics, the Eh-AgNPs were more effective against Bacillus cereus (ATCC 10876) and Candida albicans (ATCC 10231), exhibiting 9.14 ± 0.05 mm and 8.24 ± 0.05 mm zones of inhibition (ZOIs), respectively, while displaying effective inhibitory activity with ZOIs ranging from 10.98 ± 0.08 to 13.20 ± 0.07 mm against the MDR bacteria. Eh-AgNP synthesis was rapid and eco-friendly. The results showed that Eh-AgNPs are promising antimicrobial agents that can be used in the development and formulation of new drugs to curb the menace of antimicrobial resistance in pathogenic and MDR microbes.

Interlayer confinement synthesis of Ir nanodots/dual carbon as an electrocatalyst for overall water splitting

2020 ◽  
Author(s):  
Yaru Li ◽  
Yu-Quan Zhu ◽  
Weili Xin ◽  
Song Hong ◽  
Xiaoying Zhao ◽  
...  
Keyword(s):  
Water Splitting ◽  
Noble Metal ◽  
High Efficiency ◽  
Overall Water Splitting ◽  
Highly Dispersed

Rationally designing low-content and high-efficiency noble metal nanodots offers opportunities to enhance electrocatalytic performances for water splitting. However, the preparation of highly dispersed nanodots electrocatalysts remains a challenge. Herein, we...

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

scholarly journals Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 667
Author(s):  
Pavel Rudenko ◽  
Yuriy Vatnikov ◽  
Nadezhda Sachivkina ◽  
Andrei Rudenko ◽  
Evgeny Kulikov ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
High Efficiency ◽  
Biological Marker ◽  
High Sensitivity ◽  
Antagonistic Activity ◽  
Adhesive Properties ◽  
Surgical Infection ◽  
Surgical Infections ◽  
Causative Agents ◽  
Inflammatory Processes

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum “Victoria” No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum “Victoria” No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum “Victoria” No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

An insight into the determination of size and number concentration of silver nanoparticles in blood using single particle ICP-MS (spICP-MS): Feasibility of application to samples relevant to in vivo toxicology studies

2021 ◽  
Author(s):  
Isabel Abad-Álvaro ◽  
Diego Leite ◽  
Dorota Bartczak ◽  
Susana Cuello ◽  
Beatriz Gomez-Gomez ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Single Particle ◽  
Number Concentration ◽  
Biological Matrices ◽  
Icp Ms ◽  
Toxicological Studies ◽  
Insight Into

Toxicological studies concerning nanomaterials in complex biological matrices usually require a carefully designed workflow that involves handling, transportation and preparation of a large number of samples without affecting the nanoparticle...

scholarly journals Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 852
Author(s):  
Tárcio S. Santos ◽  
Tarcisio M. Silva ◽  
Juliana C. Cardoso ◽  
Ricardo L. C. de Albuquerque-Júnior ◽  
Aleksandra Zielinska ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Filamentous Fungi ◽  
Entomopathogenic Fungi ◽  
Antimicrobial Agents ◽  
Insect Pests ◽  
Insect Control ◽  
Biological Synthesis ◽  
Biological Methods ◽  
Potential Use

Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.

scholarly journals Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

RSC Advances ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 2673-2702 ◽  
Author(s):  
Anupam Roy ◽  
Onur Bulut ◽  
Sudip Some ◽  
Amit Kumar Mandal ◽  
M. Deniz Yilmaz
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Mode Of Action ◽  
Antimicrobial Agents ◽  
Recent Advances

In this review, we discuss the recent advances in green synthesis of silver nanoparticles, their application as antimicrobial agents and mechanism of antimicrobial mode of action.

scholarly journals Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature

RSC Advances ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 2804-2837
Author(s):  
Chhangte Vanlalveni ◽  
Samuel Lallianrawna ◽  
Ayushi Biswas ◽  
Manickam Selvaraj ◽  
Bishwajit Changmai ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Plant Extracts ◽  
Antimicrobial Agents ◽  
Recent Literature ◽  
Antimicrobial Activities ◽  
New Findings ◽  
Potential Applications ◽  
Research Domain

Herein, we have reviewed new findings in the research domain of the green synthesis of silver nanoparticles using different plant extracts and their potential applications as antimicrobial agents covering the literature since 2015.

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