scholarly journals Proliferative activity of salivary tumor cells

2016 ◽  
Vol 1 (5) ◽  
pp. 68-70
Author(s):  
Немчинова ◽  
Svetlana Nemchinova ◽  
Раевская ◽  
Lidiya Raevskaya ◽  
Казимирский ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Proliferative Activity ◽  
Morphological Study ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Differential Diagnostics ◽  
Early Diagnostics ◽  
Fine Needle ◽  
High Proliferative Activity

Salivary carcinomas comprise 2–3 % of malignant tumors of the head and neck. The basic method of morphological study in early diagnostics is aspiration puncture with a fine needle (FNAB). Complex histologic structure and great diversity of morphological items complicate cytologic diagnostics of salivary tumors considerably. Immunocytochemical examination must be used to reduce the errors, it enables to reveal neoplastic cells at early stages of malignization. High proliferative activity of tumor cells is one of their biological peculiarities. Accuracy in differential diagnostics between benign and malignant tumors is improved through determination of mitotic index combined with a fine needle aspiration puncture.

scholarly journals A Method to Monitor mRNA Levels in Human Breast Tumor Cells Obtained by Fine-needle Aspiration

1997 ◽  
Vol 6 (6) ◽  
pp. 353-360 ◽  
Author(s):  
Majella S. de Lange ◽  
Bert Top ◽  
Caro Lambrechts ◽  
Riks A. Maas ◽  
Hans L. Peterse ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fine Needle Aspiration ◽  
Breast Tumor ◽  
Needle Aspiration ◽  
Mrna Levels ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Breast Tumor Cells ◽  
Fine Needle

scholarly journals Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergei E. Titov ◽  
Mikhail K. Ivanov ◽  
Pavel S. Demenkov ◽  
Gevork A. Katanyan ◽  
Eugenia S. Kozorezova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Molecular Markers ◽  
Fine Needle Aspiration ◽  
Real Time Pcr ◽  
Thyroid Nodules ◽  
Nuclear Dna ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Thyroid Tumors ◽  
Fine Needle

Abstract Background Analysis of molecular markers in addition to cytological analysis of fine-needle aspiration (FNA) samples is a promising way to improve the preoperative diagnosis of thyroid nodules. Nonetheless, in clinical practice, applications of existing diagnostic solutions based on the detection of somatic mutations or analysis of gene expression are limited by their high cost and difficulties with clinical interpretation. The aim of our work was to develop an algorithm for the differential diagnosis of thyroid nodules on the basis of a small set of molecular markers analyzed by real-time PCR. Methods A total of 494 preoperative FNA samples of thyroid goiters and tumors from 232 patients with known histological reports were analyzed: goiter, 105 samples (50 patients); follicular adenoma, 101 (48); follicular carcinoma, 43 (28); Hürthle cell carcinoma, 25 (11); papillary carcinoma, 121 (56); follicular variant of papillary carcinoma, 80 (32); and medullary carcinoma, 19 (12). Total nucleic acids extracted from dried FNA smears were analyzed for five somatic point mutations and two translocations typical of thyroid tumors as well as for relative concentrations of HMGA2 mRNA and 13 microRNAs and the ratio of mitochondrial to nuclear DNA by real-time PCR. A decision tree–based algorithm was built to discriminate benign and malignant tumors and to type the thyroid cancer. Leave-p-out cross-validation with five partitions was performed to estimate prediction quality. A comparison of two independent samples by quantitative traits was carried out via the Mann–Whitney U test. Results A minimum set of markers was selected (levels of HMGA2 mRNA and miR-375, − 221, and -146b in combination with the mitochondrial-to-nuclear DNA ratio) and yielded highly accurate discrimination (sensitivity = 0.97; positive predictive value = 0.98) between goiters with benign tumors and malignant tumors and accurate typing of papillary, medullary, and Hürthle cell carcinomas. The results support an alternative classification of follicular tumors, which differs from the histological one. Conclusions The study shows the feasibility of the preoperative differential diagnosis of thyroid nodules using a panel of several molecular markers by a simple PCR-based method. Combining markers of different types increases the accuracy of classification.

Fine-Needle Aspiration Biopsies of Ovarian Masses: A Reliable Technique

2016 ◽  
Vol 60 (5) ◽  
pp. 465-474 ◽  
Author(s):  
Maral Mokhatri ◽  
Golsa Shekarkhar ◽  
Zahra Sarraf
Keyword(s):  
Diagnostic Accuracy ◽  
Fine Needle Aspiration ◽  
Malignant Tumors ◽  
Invasive Procedure ◽  
Needle Aspiration ◽  
Histological Diagnosis ◽  
Diagnostic Modality ◽  
Reliable Technique ◽  
Fine Needle ◽  
Ovarian Masses

Objective: In gynecology, fine-needle aspiration (FNA) has an overall accuracy of 94.5% in differentiation between benign and malignant tumors. The purpose of this study was to determine reliable cytological criteria for categorizing ovarian masses into benign and malignant categories, their subtypes, and also to evaluate FNA accuracy in the diagnosis of ovarian tumors in relation to histopathological findings. Study Design: A prospective study was performed on all patients with a preoperative diagnosis of ovarian tumor who were referred to our hospital between August 2013 and August 2015. During surgery, FNA was performed using an 18-gauge needle by a pathologist. Aspirated material was spread on clean glass slides and stained with Papanicolaou and Wright-Giemsa stains. The cytological findings and results were compared with the histological diagnosis. Results: Of the 81 cases in this study, there was a discrepancy between the cytological and histological diagnosis in 9 cases. The overall cytological diagnostic accuracy in our study was 88.9% with a sensitivity and specificity of 78.1 and 95.5%, respectively. Conclusion: FNA of an ovarian mass is a minimally invasive procedure with acceptable diagnostic accuracy, especially when differentiating between benign and malignant lesions, and can be considered as a useful diagnostic modality for choosing an appropriate management course.

Degenerated Keratinized Tumor Cells in Oropharyngeal Human Papilloma Virus-Associated Squamous Cell Carcinoma: A Pitfall in p16 Immunostaining of Fine-Needle Aspiration Specimens

2018 ◽  
Vol 62 (5-6) ◽  
pp. 339-345 ◽  
Author(s):  
Shuanzeng Wei ◽  
Hormoz Ehya
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Nodes ◽  
Human Papilloma Virus ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Papilloma Virus ◽  
Fine Needle ◽  
Viable Tumor

Objective: High-risk human papilloma virus (HPV) testing should be performed on all patients with newly diagnosed oropharyngeal HPV-associated squamous cell carcinoma (OPHPVSCC), and p16 immunostaining can be used as a surrogate marker. Although in surgical pathology specimens p16 staining in > 70% of the tumor cells is considered a positive result, the interpretation in fine-needle aspiration (FNA) specimens has remained controversial. Study Design: FNA of neck lymph nodes and corresponding surgical specimens from 42 patients with OPHPVSCC were reviewed. Results: In FNA specimens, 38 cases (90.5%) had viable tumor cells, 32 (76.2%) had keratin debris, and 36 (85.7%) had degenerated keratinized tumor cells. Twenty-seven of 27 (100%) had positive p16 staining in > 70% of viable tumor cells, while the degenerated tumor cells were negative. Twenty of 24 (83.3%) primary OPHPVSCC exhibited focal degenerated keratinized tumor cells and/or keratin debris. Conclusions: This study showed that the majority of the OPHPVSCC metastases in lymph nodes had degenerated keratinized tumor cells and keratin debris. Many primary OPHPVSCC also demonstrated focal keratinization and/or degeneration. The degenerated tumor cells showed no immunoreactivity to p16. The same 70% cutoff used in histologic specimens should be applied in cytologic specimens, but only the viable tumor cells should be counted.

Value of EUS-Guided Fine Needle Aspiration via Rectum for the Diagnosis of Recurrent Rectal Carcinoma and Peri-Rectal Malignant Tumors

2004 ◽  
Vol 59 (5) ◽  
pp. P219
Author(s):  
Atsushi Irisawa ◽  
Manoop S. Bhutani ◽  
Takuto Hikichi ◽  
Goro Shibukawa ◽  
Tadayuki J. Takagi ◽  
...  
Keyword(s):  
Rectal Carcinoma ◽  
Fine Needle Aspiration ◽  
Malignant Tumors ◽  
Needle Aspiration ◽  
Fine Needle
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