The study of prooxidant properties of cyanide complexes of zinc (II) while the experiment

2016 ◽  
Vol 21 (32) ◽  
pp. 23-27
Author(s):  
Пухаева ◽  
Elena Pukhaeva ◽  
Чопикашвили ◽  
Lidiya Chopikashvili ◽  
Скупневский ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Zinc Chloride ◽  
Catalase Activity ◽  
Toxic Effects ◽  
Control Group ◽  
Defence Studies ◽  
Antioxidant Defence ◽  
Zinc Compounds ◽  
Lipid Peroxidation Products ◽  
Cyanide Complexes

The aim of this paper is to study the toxic effects of zinc compounds and cyanide ions on the system of lipid peroxidation and antioxidant defence. Studies were conducted on 24 rats of Wistar line, divided into three groups (control group and two experimental, having received respectively 0.1 mg / kg of zinc chloride and cyanide complexes of zinc, calculated on the Zn2+). It was found that the administration of cyanide complexes of zinc leads to the increased level of lipid peroxidation products (methaemoglobin, hydroperoxide) on the background of the decreased activity of ceruloplasmin. In addition the content of malon dialdehyde and catalase activity does not undergo significant changes.

scholarly journals Dynamics of the Antioxidant System and Endogenous Intoxication in Patients Being Treated for Microbial Eczema

2019 ◽  
Vol 26 (4) ◽  
pp. 56-65
Author(s):  
М. M. Tlish ◽  
Е. К. Popandopulo
Keyword(s):  
Molecular Weight ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Control Group ◽  
Low Molecular Weight ◽  
Antioxidant Defence ◽  
Endogenous Intoxication ◽  
Experimental Group

Aim. The paper studies the dynamics of the indicators defi ning the antioxidant system (AOS) and endogenous intoxication in patients with microbial eczema (ME) prior to and following treatment.Materials and methods. The experimental group consisted of patients with ME (n=30) and the control group comprising somatically healthy individuals (n=30). In this study, we determined the components of the antioxidant defence system in blood plasma and erythrocyte suspension, as well as assessed endogenous intoxication.Results. The study revealed an imbalance of indicators refl ecting the severity of oxidative stress and endogenous intoxication in patients suffering from ME. Prior to receiving treatment, the experimental group, as compared to the control group, exhibited an increase in catalase activity (CAT) up to 35.93 [32.50; 38.22] mmol / (min • l) (p < 0.001) and glutathione peroxidase (GPx) up to 1194.27 [1069.50; 1375.78] μmol / (min • l) (p < 0.001). In addition, there was a decrease in the activity of superoxide dismutase (SOD) to 13.28 [11.35; 14.10] u/l (p < 0.001), glutathione reductase (GR) to 501.46 [479.77; 542.52] mmol / (min • l) (p < 0.001) and the concentration of reduced glutathione to 1.64 [1.59; 1.78] μmol / ml (p = 0.043). We observed no statistically signifi cant changes in the total antioxidant activity (TAA) of blood plasma (0.59 [0.55;0.62] mg/l; р = 0.375) and the sulphhydryl groups (0.44 [0.41;0.47] ODU; р =0.111). The patients with ME exhibited the syndrome of endogenous intoxication (SEI), accompanied by an increase in the content of medium and low molecular weight molecules in plasma to 12.19 [11.20; 13.37] arb. u. (p < 0.001), with their content in erythrocytes being relatively normal – 15.23 [14.26; 16.44] arb. u. (p = 0.005). Following the treatment, changes in the studied parameters were of a multidirectional character in relation to the control values. An increased level of CAT and GPx (р <0,001), as well as lower values of SOD and GR, continued to be observed (р < 0.001). The TAA in serum (p = 0.308), sulphhydryl groups (p = 0.111) and reduced glutathione (p = 0.107) did not differ signifi cantly from the control values. The treatment did not reverse the SEI: the content of medium and low molecular weight molecules continued to increase in both plasma and erythrocytes, as compared to the control values (p < 0.001 in both cases).Conclusion. The obtained data confi rm the failure of mechanisms underlying antioxidant defence in patients with ME. Despite the attempt of its compensation by increasing the catalase activity, SOD control values were still not reached. An increase in the GPx activity along with a decrease in GR was observed, which could have lead, in turn, to a decrease in the concentration of reduced glutathione. The failure of the antioxidant defence mechanisms is also indicated by an increase in the content of medium and low molecular weight molecules, as well as by the development of the SEI, resulting from the excessive formation of reactive oxygen species and the inability of the antioxidant system to neutralise them.

Lipid peroxidation and antielastase activity in the lung under oxidant stress: role of antioxidant defenses

1991 ◽  
Vol 70 (4) ◽  
pp. 1456-1462 ◽  
Author(s):  
V. Mohsenin
Keyword(s):  
Lipid Peroxidation ◽  
Oxidant Stress ◽  
Lavage Fluid ◽  
Conjugated Dienes ◽  
Antioxidant Defenses ◽  
Vitamin Supplementation ◽  
Control Group ◽  
Early Event ◽  
Lipid Peroxidation Products

The role of lipid peroxidation in the inactivation of alpha 1-protease inhibitor (alpha 1-PI) in the alveolar lining fluid of human subjects has been examined under oxidant stress. Exposure to nitrogen dioxide (NO2) at 4 ppm for 3 h resulted in a significant increase in the amount of lipid peroxidation products in the alveolar lining fluid, with conjugated dienes the predominant species. Four-week supplementation with vitamins C and E before NO2 exposure markedly decreased the levels of conjugated dienes (control 804 +/- 103 pmol/micrograms total phospholipids vs. vitamin-supplemented 369 +/- 58, P = 0.003). Malondialdehydes, although detectable in the lavage fluid, contributed little to the total amount of lipid peroxidation products, and the levels were comparable in both groups. NO2 exposure in the absence of vitamin supplementation caused a significant decrease in the elastase inhibitory capacity (EIC) of the alveolar lining fluid in the control group but not in the vitamin-supplemented group [control 3.67 +/- 0.32 micrograms alpha 1-PI/micrograms porcine pancreatic elastase (PPE) vs. vitamin-supplemented 2.75 +/- 0.17, P less than 0.03]. The vitamin-supplemented group had a lower level of conjugated dienes and a higher EIC. Conversely, the control group had higher levels of conjugated dienes and a lower EIC in their lavage fluid. These studies demonstrate that lipid peroxidation occurs as an early event during oxidant exposure in the lungs of normal subjects. The appearance of lipid peroxidation products in the lavage fluid is associated with a decrease in the EIC of the alveolar lining fluid. Vitamins C and E diminish lipid peroxidation and preserve the EIC of the lower respiratory tract fluid during oxidant stress.

EFFECT OF LIPID PEROXIDES ON PROSTACYCLIN AND THROMBOXANE GENERATION IN HYPERCHOLESTEROLEMIC RABBITS

1987 ◽  
Author(s):  
Wang Jian ◽  
Lu Yungcai ◽  
Zhen Erzhen ◽  
Guo Zhaozheng ◽  
Shi Fang
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Lipid ◽  
Lipid Peroxides ◽  
Atherogenic Diet ◽  
Control Group ◽  
Atherosclerotic Plaques ◽  
Platelet Aggregability ◽  
Lipid Peroxidation Products ◽  
Vessel Walls ◽  
High Level

Rabbits feeding on atherogenic diet for 60 days resulted in high level of plasma lipid peroxides as well as extreme hypercholesterolemia. Both of them kept at high level until 35 days after atherogenic diet stopped. At the same time, as compared with the control group, plasma PGI2 level was remarkably decreased while TXA2 and platelet aggregability were increased. Atherosclerotic vessel walls contain high levels of lipid peroxides associated with decreased PGI2 and increased TXA2 generation. Atherosclerotic plaques had the highest level of lipid peroxides and TXA2 while PGI production was the least, as compared with non-plaque tissue of the same artery and the normal arteries. The condition of normal arteries was just reverse. It was concluded that the concurrent presence of lipid peroxidation products may be seriously considered when evaluating the hyperlipidemia as a cause of atherosclerosis, and the elevation of TXA2 generation in arteries might be taken into consideration when evaluating an imbalance of PGI2/TXA2 in plasma.

Folic acid supplemented goat milk has beneficial effects on hepatic physiology, haematological status and antioxidant defence during chronic Fe repletion

2014 ◽  
Vol 82 (1) ◽  
pp. 86-94 ◽  
Author(s):  
María JM Alférez ◽  
Emilio Rivas ◽  
Javier Díaz-Castro ◽  
Silvia Hijano ◽  
Teresa Nestares ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Folic Acid ◽  
Goat Milk ◽  
Cow Milk ◽  
Control Group ◽  
Antioxidant Defence ◽  
Folate Supplementation ◽  
Dietary Folate ◽  
Enzymatic Antioxidant ◽  
Beneficial Effects

The aim of the current study was to asses the effect of goat or cow milk-based diets, either normal or Fe-overloaded and folic acid supplement on some aspects of hepatic physiology, enzymatic antioxidant defence and lipid peroxidation in liver, brain and erythrocyte of control and anaemic rats after chronic Fe repletion. 160 male Wistar rats were placed on 40 d in two groups, a control group receiving normal-Fe diet and the Fe-deficient group receiving low Fe diet. Lately, the rats were fed with goat and cow milk-based diets during 30 d, with normal-Fe content or Fe-overload and either with normal folic or folic acid supplemented. Fe-overload increased plasma alanine transaminase and aspartate transaminase levels when cow milk was supplied. Dietary folate supplementation reduced plasma transaminases levels in animals fed goat milk with chronic Fe overload. A remarkable increase in the superoxide dismutase activity was observed in the animals fed cow milk. Dietary folate supplement lead to a decrease on the activity of this enzyme in all the tissues studied with both milk-based diets. A concomitant increment in catalase was also observed. The increase in lipid peroxidation products levels in rats fed cow milk with Fe-overload, suggest an imbalance in the functioning of the enzymatic antioxidant defence. In conclusion, dietary folate-supplemented goat milk reduces both plasma transaminases levels, suggesting a hepatoprotective effect and has beneficial effects in situation of Fe-overload, improving the antioxidant enzymes activities and reducing lipid peroxidation.

scholarly journals CONTENT OF PRIMARY AND SECONDARY LIPID PEROXIDATION PRODUCTS IN SUBCELLULAR FRACTIONS OF CARDIOMYOCYTES DURING MYOCARDIAL INFARCTION IN RATS IN AN EXPERIMENT AND THEIR CORRECTION BY TRANSPLANTATION OF MESENCHYMAL STEM CELLS

2021 ◽  
Vol 11 (4) ◽  
pp. 89-92
Author(s):  
Vyacheslav Mykhaylichenko ◽  
Andrey Pilipchuk ◽  
Dmitry Parshin ◽  
Yuri Kostyamin
Keyword(s):  
Myocardial Infarction ◽  
Lipid Peroxidation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Subcellular Fractions ◽  
Control Group ◽  
Diene Conjugates ◽  
Mesenchymal Stem Cell Transplantation ◽  
Lipid Peroxidation Products ◽  
Group I

Experimental modeling of myocardial infarction in rats was carried out by ligation of the anterior intergastric artery after the first division. There were 3 groups of 20 animals each: control group I — to verify normal parameters, group II — a model of myocardial infarction, and group III — animals which, after modeling myocardial infarction, underwent transplantation of mesenchymal stem cells. The level of lipid peroxidation products — diene conjugates and malondialdehyde — was studied by spectrophotometry. Comparison of the content and their ratio in the cytoplasm and mitochondria of myocardiocytes was carried out. It turned out that transplantation of mesenchymal stem cells significantly levels the activation of lipid peroxidation processes in subcellular fractions of cardiomyocytes, which is accompanied by a decrease in the primary and secondary products of oxidative stress. The ratio of malondialdehyde to diene conjugates both in the cytoplasm and in the mitochondria of cardiomyocytes after transplantation returned to control values. This indicates the normalization of physiological processes with underlying ischemic heart damage. The results indicate the cytoprotective effect of mesenchymal stem cell transplantation and the preservation of a larger number of cell pools, compared with the control group of animals that did not receive any treatment.

scholarly journals Protective Effect of Dorema glabrum on Induced Oxidative Stress by Diazinon in Hippocampus of Rat

2019 ◽  
pp. 1-7
Author(s):  
Mina Adampourezare ◽  
Parisa Sistani ◽  
Homeira Hatami Nemati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Male Wistar Rats ◽  
Pre Treatment

Introduction: Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Dorema glabrum has antioxidant properties, so can be used as an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D. glabrum against DZN-induced oxidative stress in hippocampus. Methods: Twenty-four adult male Wistar rats were used in this study. The rats randomly were divided into four groups including a control group, and two groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrum usage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼ hippocampus. Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D. glabrum and DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN. Conclusion: Our study demonstrated that D. glabrum had an amelioratory effect on oxidative stress induced by DZN.

EFFECT OF LOW INTENSITY LASER THERAPY (WAVELENGTH 1265 NM) ON THE LEVEL OF ACTIVE OXYGEN AND LIPID PEROXIDATION-ANTIOXIDANT SYSTEM IN CHO-K1 AND A875 CELLS

2020 ◽  
pp. 122-132
Author(s):  
A.V. Khokhlova ◽  
L.V. Poludnyakova ◽  
A.Yu. Fedotova ◽  
D.A. Stolyarov ◽  
A.K. Gil'mutdinova ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Cell Line ◽  
Laser Therapy ◽  
Catalase Activity ◽  
Chinese Hamster ◽  
Reactive Oxygen ◽  
Infrared Laser ◽  
Oxygen Species ◽  
Antioxidant Defence

Near-infrared laser therapy has a significantly greater ability to penetrate living tissues compared to visible range optical radiation and often has the opposite effect on normal and malignant cells, thus being a promising tool for superficial neoplasm therapy. However, the mechanisms underlying the interaction between laser and biological structures can be of a different nature due to the chosen wavelength, power, and other radiation parameters. The aim of the paper is to study the efficacy of laser radiation with a wavelength of 1265 nm (9.45–18.9 J/cm2) on the generation of reactive oxygen species (ROS), the enzyme level of the antioxidant defence (AOD) and lipid peroxidation (LPO) in normal and cancer cells of Chinese hamster ovary epithelial cell line CHO-K1 and human melanoma A875. Materials and Methods. The experiment was carried out on cell lines CHO-K1 and A875, which were submitted to laser irradiation (wavelength 1265 nm, power 4.2–100 mW) for 30–60 min. Fluorescent staining with DCFH-DA was used to study intracellular ROS concentration; ImageJ software was used for image processing. The level of LPO products and the activity of AOD enzymes were determined by biochemical methods. Statistical processing of the results was carried out using the Mann–Whitney U-test (p<0.05). Results. The authors revealed an increase in ROS level, a decrease in superoxide dismutase and catalase activity, and a decrease in malondialdehyde level in CHO-K1 cells. An increase in catalase activity and malondialdehyde level was observed in A875 cells. Conclusion. Laser therapy (wavelength 1265 nm) enhances ROS generation and suppresses LPO-AOD system in the noncancerous cell line CHO-K1. However, opposite irradiation effects are observed in melanoma A875 cells. Keywords: cell culture, cancer, melanoma, low level laser therapy, infrared laser therapy, oxidative stress, mitochondria, reactive oxygen species, lipid peroxidation, antioxidant defence. Ближнее инфракрасное лазерное излучение обладает значительно большей способностью проникать в живые ткани по сравнению с оптическим излучением видимого диапазона и нередко оказывает противоположное действие на нормальные и злокачественные клетки, являясь, таким образом, перспективным инструментом для терапии поверхностных новообразований. Однако механизмы, лежащие в основе взаимодействия лазера и биологических структур, могут иметь различную природу в силу выбранной длины волны, мощности и некоторых других параметров излучения. Цель работы – исследовать влияние лазерного излучения с длиной волны 1265 нм в дозах 9,45–18,9 Дж/см2 на генерацию активных форм кислорода (АФК), уровень ферментов системы антиоксидантной защиты (АОЗ) и перекисного окисления липидов (ПОЛ) в нормальных и раковых клетках на примере линий эпителия яичника китайского хомячка CHO-K1 и меланомы человека A875. Материалы и методы. Эксперимент выполнен на клеточных линиях CHO-K1 и A875, которые облучались источниками лазерного излучения с длиной волны 1265 нм, мощностью 4,2–100 мВт в течение 30–60 мин. Внутриклеточная концентрация АФК изучалась с помощью флуоресцентного окрашивания DCFH-DA, обработка изображений осуществлялась в программе ImageJ. Уровень продуктов ПОЛ и активность ферментов АОЗ определялись биохимическими методами. Статистическая обработка результатов проводилась с использованием U-критерия Манна–Уитни (p<0,05). Результаты. В клетках линии CHO-K1 выявлено повышение уровня АФК, снижение активности супероксиддисмутазы и каталазы, а также снижение уровня малонового диальдегида. В клетках линии A875 установлено повышение активности каталазы и уровня малонового диальдегида. Выводы. Лазерное излучение с длиной волны 1265 нм способствует усилению генерации АФК и угнетению системы ПОЛ – АОЗ в нераковой клеточной линии CHO-K1, в то время как в клетках меланомы A875 наблюдаются противоположные эффекты облучения. Ключевые слова: культура клеток, рак, меланома, низкоинтенсивное лазерное излучение, инфракрасное лазерное излучение, окислительный стресс, митохондрии, активные формы кислорода, перекисное окисление липидов, антиоксидантная защита.

scholarly journals Lipid peroxidation, activity of Na+,K+-ATPase and exzymes of antioxidant defence in rats with nephropathy induced by cobalt chloride

2010 ◽  
Vol 56 (5) ◽  
pp. 540-544
Author(s):  
A.I. Tedtoeva ◽  
F.S. Dzugkoeva ◽  
I.V. Mozhaeva ◽  
S.G. Dzugkoev
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Atpase Activity ◽  
Catalase Activity ◽  
Cobalt Chloride ◽  
Male Rats ◽  
Water Reabsorption ◽  
Electrolyte Excretion ◽  
Antioxidant Defence ◽  
Renal Functions

Chronic parenteral administration of cobalt chloride (6 mg/kg) to male rats for 2 weeks or 1 month was accompanied by activation of lipid peroxidation (LPO), a decrease of superoxide dismutase activity and an increase of catalase activity. The membrane toxic action also resulted in a decrease of cortical and medullar Na+,K+-ATPase activity of kidneys, and the decrease in renal functions (glomerular filtration, renal water reabsorption, spontaneous diuresis, electrolyte excretion).

scholarly journals Effect of iron nanoparticles on free radical oxidation process in blood of rats with Pliss lymphosarcoma

2016 ◽  
Vol 62 (5) ◽  
pp. 555-560 ◽  
Author(s):  
P.S. Kachesova ◽  
I.A. Goroshinskaja ◽  
V.B. Borodulin ◽  
E.V. Shalashnaja ◽  
A.V. Chudilova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Normal Values ◽  
Biological Effects ◽  
Free Radical Oxidation ◽  
Main Group ◽  
Careful Examination ◽  
Control Group ◽  
Sod Activity ◽  
Lipid Peroxidation Products ◽  
Intact Rats

The use of metal nanoparticles (NPs) for cancer treatment requires careful examination of their biological effects. The aim of this study was to determine parameters of oxidative processes in the blood of tumor-bearing animals treated with metallic iron NPs only. The markers of antioxidant status and accumulation of lipid peroxidation products were measured in erythrocytes and blood plasma of rats with Pliss lymphosarcoma (PLS) and intact rats. PLS animals were treated eight times with iron NPs (at a dose of 1.25 mg/kg bw (main group), rats of the control group received saline (0.3 ml). In control animals, an increase in malondialdehyde (MDA) was observed in red blood cells (RBC) by 45%; this was accompanied by compensatory increase in reduced glutathione (GSH) and catalase by 24% and 14.3%, respectively (p<0.05). In plasma an increase in MDA by 167.4% (p<0.01) and a decrease in oxidase activity of ceruloplasmin (CP) by 36.8% (p<0.001) were found. In the main group there was a decrease of accumulation of lipid peroxidation products in the blood. Intensity of detected changes depended on the antitumor effect: rats with growing LSP showed a tendency to the decrease in the RBC MDA level and normalization of plasma MDA; in animals with LSP regression this marker did not differ from normal values. In all animals of the main group the CP content was basically the same as in intact rats while GSH increased in the group without therapeutic effect (by 218.6%) and in the group with the effect by 69% (versus normal values; p<0.01). SOD activity in the rats with LSP growth significantly increased (by 42%), in the rats with regression decreased (by 30%) with subsequent normalization. Thus, administration of iron NPs caused activation of the antioxidant system in blood and a significant decrease in the manifestations of oxidative stress associated with tumor growth.

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