INDICES OF IMMUNE RESPONSES OF BULLS IN THE NECROBACILLOSIS VACCINATION OF THE CATTLE

2015 ◽  
Vol 10 (3) ◽  
pp. 93-97
Author(s):  
Михеева ◽  
Ekaterina Mikheeva ◽  
Бабинцева ◽  
Tatyana Babintseva ◽  
Макаев ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Immune Responses ◽  
Blood Serum ◽  
B Lymphocytes ◽  
Metabolic Disorders ◽  
Specific Antibodies ◽  
Biological Safety ◽  
Digestive Organs ◽  
Udmurt Republic ◽  
And Control

Metabolic disorders and dysfunctional state of the digestive organs lead to the development of distal extremities diseases. The greatest danger is neсrobacrillosis. Vaccination is one of the ways to prevent and control this disease. The article reflects the data on the effect of vaccination (“Nekovak” and formol-emulsion neсrobacrillosis vaccine of Federal Centre for toxicology, radiation and biological safety) on immunological and serological parameters of blood and blood serum of cattle in the Udmurt Republic. After immunization with “Nekovak” vaccine and formol-emulsion neсrobacrillosis vaccine, we determined an increase of the number of leukocytes to 7th day, and also an increase of T-lymphocytes to 14th day. The number of B-lymphocytes reaches a maximum on 21st day. The content of gamma globulins, including specific antibodies against the neсrobacrillosis pathogen, exceeded the control and reached a maximum on the 21st day after vaccination.

scholarly journals Isotype commitment in the in vivo immune responses. I. Antigen-dependent specific and polyclonal plaque-forming cell responses by B lymphocytes induced to extensive proliferation.

1982 ◽  
Vol 156 (3) ◽  
pp. 690-702 ◽  
Author(s):  
M Björklund ◽  
A Coutinho
Keyword(s):  
Immune Responses ◽  
B Lymphocytes ◽  
Antibody Responses ◽  
Regulatory Mechanisms ◽  
Transfer System ◽  
Spleen Cells ◽  
Serial Transfer ◽  
Specific Antibodies ◽  
Cell Responses

The random recombination and deletion hypothesis for the control of isotype commitment in antibody responses was directly tested in a serial transfer system in vivo. Normal or hyperimmune spleen cells were used in weekly serial transfers with antigen into irradiated recipients until clonal senescence was observed. Antigen-specific and -nonspecific plaque-forming cells of all isotypes were determined at each transfer time. No major changes in the isotypes of specific antibodies were observed for the whole life-span of the transferred cells (9-10 wk), and no indication was obtained for the accumulation of cells transcribing the most 3' members of the C-gene cluster with sustained proliferation. Rather, the dominant isotypes were found throughout the response to be IgG1, IgG2b, and IgG2a. The results imply isotype-specific regulatory mechanisms in the control of Ig class production. These appear to operate as well in the antigen-nonspecific component of the immune response.

The neonatal immune system: modulation by regulatory T-cells and CTLA-4 (CD152)1)

2013 ◽  
Vol 37 (3) ◽  
Author(s):  
Katrin Hebel ◽  
Mandy Pierau ◽  
Holger Lingel ◽  
Michael Steiner ◽  
Hardy Krause ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Regulatory T Cells ◽  
Immune Responses ◽  
B Lymphocytes ◽  
Neonatal Immune System ◽  
Immune System Modulation ◽  
B And T Lymphocytes ◽  
Differentiation Of Lymphocytes

AbstractThe dysregulation of CTLA-4 (CD152), a glycoprotein expressed on the surface of lymphocytes, may lead to chronic inflammation. Based on the recent scientific findings, it has become clear that CTLA-4 inhibits the effector function and, thus, shuts down the effector phase of T-lymphocytes. Interestingly, the CTLA-4-expressing cells become resistant to apoptosis (programmed cell death) and increasingly migrate to the lymph nodes and tissues. Studies have shown that regulatory T cells (Tregs), which switch off unwanted immune responses can inhibit in vivo only if they express an intact CTLA-4 gene. Moreover, it was confirmed that CTLA-4 is not only expressed on T-lymphocytes but also on B-lymphocytes. The mice with genetic inactivation of CTLA-4 show in B-lymphocytes an increased production of IgM antibodies after immunization. Interestingly, in particular, B- and T-lymphocytes from newborns and infants show a strongly increased CTLA-4 expression, suggesting a key immunoregulatory role in neonatal immune responses. Molecules such as CTLA-4, which regulate the differentiation of lymphocytes, could provide therapeutic targets during the early childhood to set the course for protection against autoimmunity and allergy.

scholarly journals State of the immune system in women using the “Provag” probiotic

2020 ◽  
Vol 22 (4) ◽  
pp. 785-790
Author(s):  
V. A. Kozlov ◽  
A. G. Borisov ◽  
A. A. Savchenko ◽  
A. E. Kondakov ◽  
I. V. Kudryavtsev
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Lactobacillus Plantarum ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Lactobacillus Fermentum ◽  
Passive Hemagglutination ◽  
Specific Antibodies ◽  
Lactobacillus Gasseri ◽  
Healthy Women

Lactobacilli are  widely used  in clinical  practice as probiotics, biologically  active  additives  and probiotic products for functional nutrition. Some  probiotics can  be considered as bacterial vaccines  due  to induction of immune response, accompanied by production of specific antibodies. The aim of the present study was to evaluate  the state of cellular and humoral immunity in women  by using probiotic strains of lactobacilli. The  study  included 31 healthy  women  aged  25-45  years.  As a source  of probiotic lactobacterial complex, we used  the  “Provag” preparation (RU  77.99.11.003.E.003746.02.11 of 11.02.2011, 1 capsule  contains 109 Lactobacillus  gasseri 57C,  Lactobacillus  fermentum  57A и Lactobacillus  plantarum  57B).  The  drug  was used for 30 days, at a rate  of one  capsule  per day. The  immune system was examined twice: before  administering the drug and after 30 days of treatment. The study of blood  lymphocyte populations and subpopulations was performed by flow cytometry using direct immunofluorescence technique. The concentration of IgA, IgM, IgG in blood serum was determined using enzyme  immunoassay. To determine specific antibodies, we used passive hemagglutination reaction with erythrocyte diagnosticum. The complex of probiotic lactobacilli Lactobacillus gasseri, Lactobacillus  fermentum  and  Lactobacillus  plantarum corresponding to the “Provag” preparation was used as a source  of antigen. It has been revealed  that  the number of T and B lymphocytes in peripheral blood increased after  30 days  of treatment with  the  probiotic preparation “Provag” in  healthy  women. Elevated contents of T cells was due to the T helper  cell fraction. Increased levels of T helpers and B lymphocytes were associated with stimulation of humoral immunity, as evidenced by increasing concentration of IgA and  IgG in blood  serum.  By means  of passive hemagglutination reaction, we have found  that  90% of healthy  women showed increased concentrations of specific IgA in blood after 30 days of treatment with “Provag” preparation.

scholarly journals Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory Response in Male Rats with Different Resistance to Hypoxia

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Dzhuliia Sh. Dzhalilova ◽  
Anna M. Kosyreva ◽  
Mikhail E. Diatroptov ◽  
Natalia A. Zolotova ◽  
Ivan S. Tsvetkov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Inflammatory Response ◽  
Blood Serum ◽  
Nk Cells ◽  
B Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Peripheral Blood ◽  
Absolute Number ◽  
Resistance To Hypoxia ◽  
Functional Changes

On the model of the systemic inflammatory response (SIRS), induced by lipopolysaccharide (LPS), the morphological and functional changes in the thymus and spleen and the subpopulation composition of peripheral blood lymphocytes of rats differing in resistance to hypoxia were studied. It was demonstrated that the level of endotoxin in blood serum after 3 hours of LPS administration in susceptible-to-hypoxia rats was 64 times higher than in the control group, while in tolerant-to-hypoxia animals it was only 8 times higher in 6 hours. After 24 hours of LPS injection, only in susceptible-to-hypoxia rats did the level of C-reactive protein in blood serum increase. There is a difference in the dynamics of morphological changes of lymphoid organs after LPS injection in tolerant- and susceptible-to-hypoxia animals. After 3 hours of LPS administration, the tolerant-to-hypoxia rats showed no changes in the thymus, spleen, and subpopulation composition of lymphocytes in peripheral blood. After 6 hours there was only a decrease in B-lymphocytes and increase in cytotoxic T-lymphocytes and NK cells. After 1 day of LPS injection, the tolerant-to-hypoxia rats had devastation in PALS of the spleen. After 3 hours of LPS injection the susceptible-to-hypoxia animals had reactive changes in the lymphoid organs: decrease of the thymus cortex, narrowing of the marginal zones of spleen lymphoid follicles, widening of their germinal centers, and a decrease in the absolute number of cytotoxic T-lymphocytes, NK cells, and B-lymphocytes. After 24 hours of LPS injection the tolerant-to-hypoxia animals had a greater absolute number of T-lymphocytes and NK cells in comparison with the susceptible rats. Thus, in animals with different resistance to hypoxia the LPS-induced SIRS is characterized by different dynamics of morphological and functional changes of the thymus and spleen. The obtained data will serve as a basis for the development of new individual approaches to the prevention and treatment of infectious and inflammatory diseases.

scholarly journals High levels ofbcl-2 protein in circulating t lymphocytes, but not b lymphocytes, of patients with systemic lupus erythematosus

1994 ◽  
Vol 37 (10) ◽  
pp. 1423-1430 ◽  
Author(s):  
Martin Aringer ◽  
Winfried Wintersberger ◽  
Carl W. Steiner ◽  
Hans Kiener ◽  
Elisabeth Presterl ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Lupus Erythematosus ◽  
Systemic Lupus

Association between Vaspin rs2236242 Gene Polymorphism and Psoriasis Vulgaris

2020 ◽  
pp. 1-6
Author(s):  
Nazli Dizen-Namdar ◽  
Raziye Akcilar ◽  
Zeynep Bayat
Keyword(s):  
Gene Polymorphism ◽  
Metabolic Disorders ◽  
Psoriasis Vulgaris ◽  
Turkish Population ◽  
Inflammatory Skin Disease ◽  
Control Groups ◽  
Diagnostic Biomarkers ◽  
Increased Risk ◽  
Visceral Adipose ◽  
And Control

<b><i>Background:</i></b> Psoriasis known as a chronic inflammatory skin disease is accompanied by metabolic disorders such as obesity, diabetes, and dyslipidemia. Vaspin (a serine protease inhibitor derived from visceral adipose tissue) is a newly identified adipokine and a link between inflammation and obesity has been reported. We aimed to determine whether vaspin gene polymorphism is associated with the development and/or clinical features of psoriasis vulgaris. <b><i>Methods:</i></b> Our study group consisted of 96 psoriasis vulgaris patients and 100 matched controls. Vaspin rs2236242 gene was genotyped using PCR. <b><i>Results:</i></b> The vaspin genotypes showed a meaningful difference between psoriasis and control groups (<i>p</i> = 0.02). The frequency of the vaspin rs2236242 TT genotype was lower in psoriasis patients than in control participants (<i>p</i> &#x3c; 0.05). The TA genotype was associated with a 2.38-fold increased risk of psoriasis compared to the TT genotype (<i>p</i> = 0.007, odds ratio: 2.38; 95% confidence interval: 1.25–4.55), but not the AA genotype. All subjects were the Turkish population, the study in other populations is needed and the sample size was small in number. <b><i>Conclusion:</i></b> Our study demonstrated that vaspin rs2236242 polymorphism is related to psoriasis in the Turkish population. Polymorphisms of the vaspin gene might serve as diagnostic biomarkers of psoriasis.

scholarly journals A REPORT ON ANTIMENINGITIS VACCINATION AND OBSERVATIONS ON AGGLUTININS IN THE BLOOD OF CHRONIC MENINGOCOCCUS CARRIERS

1918 ◽  
Vol 28 (4) ◽  
pp. 449-474 ◽  
Author(s):  
Frederick L. Gates
Keyword(s):  
Blood Serum ◽  
Salt Solution ◽  
Blood Stream ◽  
Severe Reaction ◽  
Meningeal Irritation ◽  
Specific Antibodies ◽  
Individual Susceptibility ◽  
Normal Controls

1. A meningococcus vaccine suspended in salt solution has been given subcutaneously as a prophylactic to about 3,700 volunteers in three injections of 2,000 million, 4,000 million, and 4,000 or 8,000 million cocci at weekly intervals. 2. These doses rarely caused more than the mildest local and general reactions. Exceptionally a more severe reaction emphasized the presence of an unusual individual susceptibility to the vaccine. In such instances the symptoms were in part those of meningeal irritation and sometimes simulated the onset of meningitis. 3. Specific meningococcus agglutinins have been demonstrated in the blood serum of vaccinated men as compared with normal controls. 4. Moreover, agglutinins have been demonstrated in the blood serum of chronic carriers of the meningococcus. Evidence is thus brought forward that the relative immunity of chronic carriers to epidemic meningitis may be due to the presence of specific antibodies in the blood stream.

scholarly journals T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation.

1981 ◽  
Vol 153 (4) ◽  
pp. 871-882 ◽  
Author(s):  
H Y Tse ◽  
J J Mond ◽  
W E Paul
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Antigen Specificity ◽  
Apparent Lack ◽  
The Face ◽  
Stimulation Of

For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.

scholarly journals Role for CD4+ CD25+ Regulatory T Cells in Reactivation of Persistent Leishmaniasis and Control of Concomitant Immunity

2004 ◽  
Vol 200 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Susana Mendez ◽  
Stacie K. Reckling ◽  
Ciriacco A. Piccirillo ◽  
David Sacks ◽  
Yasmine Belkaid
Keyword(s):  
Immune Responses ◽  
Leishmania Major ◽  
Parasite Clearance ◽  
Effector Memory ◽  
Memory Response ◽  
Secondary Challenge ◽  
Concomitant Immunity ◽  
Disease Reactivation ◽  
And Control ◽  
Parasite Leishmania

Reactivation of dormant infections causes an immense burden of morbidity and mortality in the world at large. Reactivation can occur as a result of immunosuppression, environmental insult, or aging; however, the cause of reactivation of such infections is often not clear. We have previously shown that persistence of the parasite Leishmania major is controlled by endogenous CD4+ CD25+ regulatory T (T reg) cells. In this report, we show that despite efficient parasite clearance at secondary sites of infection, Leishmania superinfection can cause disease reactivation at the primary site. Our results strongly suggest that T reg cells, whose numbers increase in sites of reactivation, are directly responsible for such reactivation. Depletion of CD25+ cells at the time of secondary challenge prevented disease reactivation at the site of persistent infection while strengthening the expression of immunity at the site of secondary challenge. Finally, transfer of T reg cells purified from infected mice into chronically infected mice was sufficient to trigger disease reactivation and prevent the expression of an effector memory response. Our results demonstrate that after persistence is achieved, an equilibrium between T reg cells and effector lymphocytes, which can be disturbed by superinfection, controls the efficiency of recall immune responses and disease reactivation.

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