The insulin effects on the morphological structure of the kidneys in wistar rats

2015 ◽  
Vol 9 (2) ◽  
pp. 0-0
Author(s):  
Макишева ◽  
R. Makisheva ◽  
Субботина ◽  
Tatyana Subbotina
Keyword(s):  
Negative Impact ◽  
Morphological Changes ◽  
Venous Blood ◽  
Morphological Structure ◽  
Renal Parenchyma ◽  
Venous Congestion ◽  
Control Group ◽  
Insulin Effect ◽  
Hydropic Degeneration ◽  
White Rats

The study of morphological changes in the kidneys of white rats after intramuscular injection of insulin at a dose of 1 IU/kg of body weight was carried out. The insulin effect was amplified using stress models of immobilization and swimming. Blood samples from tail vein of rats were taken to confirm the action of insulin. At autopsy, the authors conducted the fence venous blood flowing from the kidneys. Comparison with a control group showed that morphological changes in the renal parenchyma were formed after the insulin introduction and characterized by venous congestion in the microvasculature, ischemic changes in the renal parenchyma with areas of hemorrhage, thickening of the basement membrane, expansion of tubules, hydropic degeneration of the epithelium, hypertrophy and enlargement of the loops of the glomeruli. Measurement of glucose in venous blood flowing from the kidneys revealed hyperglycemia. The morphological changes in the tissues of the kidney indicate the development of venous congestion, glomerular hyperfiltration and hypovolemia, hypertension, glycosuria, glycosylation membranes, the deposition of glycogen in the epithelium of kidney tubules, microalbuminuria. The obtained results allow to draw attention to the fact that among the factors of damage during the development of diabetic nephropa-thy, a hyperinsulinemia may have a negative impact

scholarly journals Morphological alterations in the heart and aorta of rats treated with glucocorticoids

2015 ◽  
Vol 32 (04) ◽  
pp. 231-235
Author(s):  
R. Dantas ◽  
K. Souza ◽  
D. Santos ◽  
V. Feitosa ◽  
E. Fioretto ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Histological Analysis ◽  
Saline Solution ◽  
Morphological Changes ◽  
Morphological Structure ◽  
Control Group ◽  
Morphological Alterations ◽  
Heart Chamber ◽  
Male Wistar Rats ◽  
Synthetic Glucocorticoid

Abstract Introduction: The objective of this study was to analyze the morphological structure of the heart and aorta of rats treated with the synthetic glucocorticoid dexamethasone. Material and Methods: Male Wistar rats were divided into two groups: 08 control rats undergoing treatment with a 0.9% saline solution for 10 days and 08 rats treated for 10 days with dexamethasone (2mg/kg animal weight). Results: Histological analysis detected a mild cardiac hypertrophy and 15% reduction of collagen located in the aorta of animals treated with glucocorticoid when compared to the control group. Conclusion: We conclude that treatment with dexamethasone for a period of 10 consecutive days is able to promote morphological changes in the structure of the heart chamber and, impair morphological structure of aorta.

scholarly journals Features of the morphological structure of the placenta in pregnant women with preeclampsia

2021 ◽  
pp. 10-15
Author(s):  
Volodymyr I. Chermak ◽  
Ihor I. Chermak ◽  
Chukwuanyinonso O. Ikeotuonye ◽  
Anatolii Ya. Senchuk
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
High Frequency ◽  
Morphological Changes ◽  
Morphological Structure ◽  
Morphological Features ◽  
Placental Insufficiency ◽  
Control Group ◽  
Basic Group ◽  
Adaptive Reactions

To study morphological features of the placenta in women in labor, pregnancy which complicated by preeclampsia we studied 26 placentae, of which 17 were from pregnant women with mild to moderate preeclampsia (basic group) and 9 from healthy women (control group). Histologically the study was conducted with methodically recommendations of T.D. Zadorozhnaya et al. for light microscopy. During the study of morphological features of the placenta in women in labor with preeclampsia were installed morphological changes in the placenta, which reflect a tendency to develop compensatory-adaptive reactions following their stress and exhaustion, which is a sign of placental insufficiency. Received data allow claiming a high frequency of development placental insufficiency in pregnant women with risk factors such as chronic salpingo-oophoritis, anemia, late preeclampsia, and inflammation diseases kidney.

scholarly journals APPLICATION OF CELLULAR TECHNOLOGIES FOR REGENERATION OF PERIODONTAL TISSUES IN EXPERIMENT

2018 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Тatiana E. Vladimirskaya ◽  
Vasilina A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Morphological Changes ◽  
Fibrous Tissue ◽  
Laboratory Animals ◽  
Control Group ◽  
Cell Transplant ◽  
Periodontal Tissues ◽  
White Rats

A promising scope of modern scientific research is the use of autologous and allogeneic mesenchymal stem cells for regeneration of periodontal tissues. The aim of the study was to evaluate the nature of morphological changes in the pathologically altered periodontal tissues after injection of a biotransplant containing mesenchymal stem cells of the adipose tissue immobilized on a collagen carrier in an animal experiment. In the experiment, 60 randobbred females of white rats were used as a model, whose adipose tissue was taken to obtain allogenic mesenchymal stem cells. All animals were divided into 5 groups, depending on the planned method of treatment – 10 rats each. The control group consisted of 10 laboratory animals with healthy gingiva. The experimental gingival recession model was created by the V-shaped excision of periodontal tissues. The bioplastic collagen material “Collost” gel 7 % in isolated form determines the fibrosis intensification and serves as a “matrix” for the formation of a fibrous tissue, ensures the adhesion of stem cells and their transformation into pro- and fibroblasts. Injection of a cell transplant suspension into physiological saline activates the processes of cell proliferation and transformation of fibroblast differentiating cells. Suspension of the cell transplant on a sterile bioplastic collagen material “Collost” gel 7 % enhances the effects of gel and stem cells, promotes the leveling of atrophic and dystrophic changes in the gum, strengthening a mechanical component, reducing the recession of the gum and the depth of the gingival pocket.

scholarly journals THE INFLUENCE OF OZONE THERAPY ON THE MORPHOLOGIC CHANGES IN THE PATIENTS PRESENTING WITH PURULENT INFLAMMATION OF THE LUNGS IN THE EXPERIMENT

2017 ◽  
Vol 16 (3) ◽  
pp. 137-140
Author(s):  
Gulora A. Sadykova ◽  
Kh. U Rakhmatullaev ◽  
R. Sh Mavlyan-Khodjaev ◽  
Z. S Zalyalova ◽  
Yu. Kh Tadjikhodjaeva
Keyword(s):  
Respiratory Tract ◽  
Chronic Inflammation ◽  
Saline Solution ◽  
Negative Impact ◽  
Morphological Changes ◽  
Laboratory Animals ◽  
Control Group ◽  
Experimental Animals ◽  
Mechanical Irritation ◽  
Inflammation Of The Lungs

We have created the experimental model of chronic inflammation of lungs by means of prolonged mechanical irritation of the bronchi in 30 outbred rats and studied the morphological changes in the lung tissue of these animals rats in three series of experiments. Each rat was given an intraperitoneal injection of an ozonised saline solution produced by a «Binafsha» ozonator. The objective of the study was to compare a control group of healthy animals and the group of experimental animals with chronic purulent pneumonia. The prolonged irritation of the respiratory tract in experimental animals was found to induce the structural changes in the tissues of the lungs characteristic of chronic purulent inflammation. The course of treatment with the ozonised saline solution in healthy animals with experimentally modelled chronic purulent inflammation of the lungs did not have a negative impact on the general condition and the behaviour of the animals. The treatment of experimental chronic inflammation of lungs caused by prolonged mechanical irritation of the respiratory tract resulted in the improvement of the morphological status of the laboratory animals, but the purulent inflammation process failed to be completely resolved after the treatment which needs to be taken into consideration in the clinical practice.

scholarly journals Hydropic degeneration of the sensorimotor cortex of white rats in the context of the formation of dark neurons and changes in neuroglial relationships following short-term common carotid arteries occlusion

2021 ◽  
pp. 66-81
Author(s):  
A.V. Gorbunova ◽  
◽  
S.S. Stepanov ◽  
V.A. Akulinin ◽  
D.B. Avdeev ◽  
...  
Keyword(s):  
Sensorimotor Cortex ◽  
Carotid Arteries ◽  
Control Group ◽  
Numerical Density ◽  
Relative Area ◽  
Hydropic Degeneration ◽  
Functional Changes ◽  
Dark Neurons ◽  
White Rats ◽  
Common Carotid Arteries

Introduction. Neurodegenerative processes are key in the development of a number of diseases — strokes, Parkinson’s and Alzheimer’s diseases, epilepsy, and traumatic brain injury. They include primary and secondary structural and functional changes in the nervous tissue, as well as the death of neurons and complete loss of functions. Aim. To study the manifestations of hydropic degeneration and reorganization of glio-cytoarchitectonics during the formation of dark neurons in the sensorimotor cortex (SMC) of the cerebrum of mature white rats in 40 min after the common carotid arteries occlusion (CCAO). Materials and methods. A 40-minute CCAO was simulated in white Wistar rats. The brain was fi xed by perfusion method. Morphometric assessment of manifestations of edema — swelling, cyto- and glio-cyto-architectonics of SMC was performed normally (n = 6, control group), on the 1st (n = 6), 3rd (n = 6) and 7th days (n = 6) after CCAO. The Nissl staining, hematoxylin and eosin, immunohistochemical typing NSE, MAP-2, GFAP and AIF1 were applied. The relative area of edema-swelling zones, the numerical density of normochromic and dark pyramidal neurons, oligodendrocytes (OD), microgliocytes (MG) were determined. Results. The high content (20–50%) of dark neurons after CCAO was accompanied by a 3.3-fold increase in the relative area of edema-swelling zones (r = 0.82, p = 0.01). Specifi c proteins (NSE, MAP-2) of most dark neurons were preserved. The total numerical density of SMC neurons decreased by 26.4% (layer III, p = 0.001) and 18.5% (layer V, p = 0.01) after 7 days of CCAO. The content of astrocytes, MG and OD increased. The peak in the number density of MG was observed in the 1st day, and OD — in the 7th day after acute subtotal ischemia (p ≤ 0.001). The revealed changes were of a diffuse-focal nature. Conclusion. After a 40-minute CCAO, the content of dark neurons in SMC increased and, as a result, signs of hydropic degeneration appeared. Against this background, the number of satellite OD, astrocytes and MG increased. Probably, edema-swelling, active MG and astrocytes previously (on the 1st – 3rd day) sanitate the nerve tissue, ensuring its subsequent (on the 7th day) structural and functional recovery with the participation of OD

scholarly journals THE IMPACT OF CENTRAL DEPRIVATION OF TESTOSTERONE ON THE STRUCTURE OF RED BONE MARROW IN MONOCYTOPOESIS IN THE EARLY TERMS OF THE EXPERIMENT

2021 ◽  
Vol 21 (2) ◽  
pp. 142-146
Author(s):  
R.V. Martynenko
Keyword(s):  
Bone Marrow ◽  
Morphological Changes ◽  
Hematopoietic Cells ◽  
Test Group ◽  
Control Group ◽  
Hematopoietic Tissue ◽  
Red Bone Marrow ◽  
White Rats ◽  
The Impact ◽  
Testosterone Synthesis

The effect of androgen deficiency on haematopoiesis has not been sufficiently studied yet. It is known that androgens have a double effect on the hematopoietic system. Hematopoietic tissue is a continuously regenerating dynamic system; therefore, the issues of hematopoietic kinetics and its regulation are key ones to understand the pathogenesis of blood diseases in general. It has been determined that the development of all hematopoietic cells results from the proliferation and differentiation of a single pluripotent blood stem cell under the influence of surrounding cells. The effect of prolonged central deprivation of testosterone synthesis on morphological changes in hematopoietic organs is still not enough described in the scientific literature. The aim of this study is to evaluate the microscopic structure of hematopoietic cells in rats during the central deprivation of testosterone synthesis by diferelin injection on the 30 day of the experiment. Materials and methods. The experiment was performed on 20 adult male white rats divided into 2 groups of 10 animals in each group: control and test group. The animals in the experimental group were injected with diferelin (Triptorelin embonate) subcutaneously in a dose of 0.3 mg of active substance per kg / body wt. The rats in the control group received a saline injection. The experiment lasted 30 days. Results. The morphological study of the red bone marrow in the test group of animals on the 30 day of the experiment has demonstrated the structure of the organ is represented by two components: stromal and parenchymal. The stroma consists of reticular cells forming a network of reticular fibbers, most of which are threaded with sinusoidal capillaries. In the middle of the capillaries there is a dense arrangement of erythrocytes in the form of a "column of coins". The pericapillary space is filled with hematopoietic cells at different stages of differentiation. The monoblastic clone is represented by monocyte cells, promonocytes, monoblasts, and progenitor cells that are of the same mophrological structure and structure under light microscopy.

Structural and Morphological Changes in the Liver Due to Intestinal Endotoxins

2020 ◽  
Vol 15 (3) ◽  
pp. 205-213
Author(s):  
Alexander L. Malev ◽  
Anna N. Zakharova ◽  
Vitalii B. Kaliberdenko ◽  
Tatyana A. Fominykh ◽  
Shanmugaraj Kulanthaivel ◽  
...  
Keyword(s):  
Structural Changes ◽  
Morphological Changes ◽  
The Body ◽  
Oxidative Modification ◽  
Oxidation Products ◽  
Control Group ◽  
Potent Inducer ◽  
Hydropic Degeneration ◽  
Oxidation Stress ◽  
Lipid Oxidation Products

Background: Under normal physiological conditions, endotoxin (ET) released during self-renewal of the colibacillus pool is an obligate stimulus for the formation of the immune system and homeostasis of the body. Violation of the barrier function of the intestinal wall and the mechanisms of neutralization of endotoxin lead to systemic endotoxemia of intestinal origin. Its development is facilitated by stress, intoxication, a decrease in nonspecific resistance of the body, as well as damage to the intestinal mucosa and dysbiosis, where the mucous membrane is more vulnerable and permeable to endotoxin. Purpose of the Research: The aim of this study is to compare and assess the severity and nature of hepatocyte damage from endotoxin exposure and the degree of manifestation of stress due to oxidation, to determine the characteristics of structural changes in hepatocytes and to assess the oxidation stress during endotoxin intoxication in the experiment with biochemical markers. Materials and Methods: The experiments were conducted on 40 non-linear rats, divided into two groups of 20 animals. Group 1 animals received intraperitoneal injections of ET of Escherichia coli drug (Sigma USA K-235) for seven days at a rate of 0.1 mg/kg of the body weight. Animals of the second group served as the control group. Character and stage of liver damage were studied using morphological methods, including electron and light microscopy. In studying oxidizing stress, biochemical methods were used to define the changes, such as conjugated dienes and dienketones, spontaneous oxidizing modification of proteins. Results and Conclusion: 1. The severity and depth of morphological changes in the liver during endotoxin intoxication were correlated with the dynamics of the content of lipid oxidation products (CD and DK, MDA) and proteins. There was a tendency for a more significant increase in the oxidative modification of proteins in serum. This confirms the data on the primary damage of proteins by free radicals. 2. When exposed to intestinal microflora endotoxin, pronounced dyscirculatory changes, fatty and hydropic degeneration of hepatocytes with signs of toxic damage to their nuclei were determined, but at the same time, the increased hyperplastic activity of sinusoidal cells remained associated with the effects of endotoxin. These changes are associated with both the direct toxic effect of endotoxin, and the effects of oxidative stress, in which endotoxin is a potent inducer.

scholarly journals Efek Protektif Penggunaan Antiradiasi Alpha Spin Terhadap Histologi Hipokampus Tikus Putih (Rattus novergicus) Galur Sprague dawley yang Terpapar Gelombang Elektromagnetik Ponsel

2020 ◽  
Vol 2 (1) ◽  
pp. 88-95
Author(s):  
Ahmad Rizki Dwi Prasetia ◽  
Waluyo Rudiyanto ◽  
Merry Indah Sari
Keyword(s):  
Treatment Group ◽  
Protective Effect ◽  
Electromagnetic Radiation ◽  
Rattus Norvegicus ◽  
Electromagnetic Waves ◽  
Pyramidal Cells ◽  
Control Group ◽  
Sprague Dawley ◽  
Hydropic Degeneration ◽  
White Rats

Exposure to cellphone electromagnetic radiation can cause oxidative stress which can later lead to changes in the hippocampal pyramidal cells. This change can be characterized by a reduction in the number of pyramidal cells and a change in cell morphology characterized by occurrence of picnosis or hydropic degeneration in the hippocampal pyramidal cell nucleus. Alpha Spin can inhibit cell phone electromagnetic radiation exposure through the quantum energy field formed by Alpha Spin. This study aims to determine the protective effect of the use of Alpha spin antiradiation on the histology of the hippocampus of white rats (Rattus norvegicus) Sprague dawley strains exposed to mobile electromagnetic waves. Laboratory experimental research using randomized controlled methods with a post test only control group design. Samples were 30 white rats divided into 3 groups. The control group (K) was only given food and drink, the treatment group 1 (P1) was exposed to cellphone electromagnetic waves and the treatment group 2 (P2) was exposed to cellphone electromagnetic waves and placed Alpha Spin. Exposure is done 2 hours / day for 30 days. There were mean differences in all groups with a significant value, which was p <0.05. There is a protective effect of Alpha Spin antiradiation on the histology of the hippocampus white rat (Rattus norvegicus) Sprague dawley strain that is exposed to cellphone electromagnetic waves.

scholarly journals THE MORPHOLOGICAL STRUCTURE OF THE LIVER AND ITS FUNCTIONAL STATE AT MEDICINAL DAMAGE AND TREATMENT BY THE NEW PHARMACEUTICAL COMPOSITION

2018 ◽  
Vol 26 (2) ◽  
pp. 12-16
Author(s):  
M. A. Morozov ◽  
E. V. Blinova ◽  
G. A. Ovsepyan ◽  
S. S. Dydykin ◽  
D. S. Blinov ◽  
...  
Keyword(s):  
Functional Activity ◽  
Morphological Structure ◽  
The Body ◽  
Synthetic Activity ◽  
Control Group ◽  
Histological Structure ◽  
Drug Induced ◽  
Intact Group ◽  
Blood Indices ◽  
White Rats

The study presented results of exploration of liver functional activity and morphological structure on the experimental model of drug-induced hepatitis by twice administration of 1000 mg/kg acetaminophen to nonlinear both sexes white rats treated by preventive administration of novel pharmaceutic formulation with laboratory code LHT-8-16. The substance was administered to the animals intra-gastrically 1 hour prior to acetaminophen intake. We studied changes in biochemical blood indices, which characterize the functional activity of the liver, assessed the organ histological structure, and performed macro- and micro-morphometric analysis. It has been shown that the substance LHT-8-16 prevents the toxic effect of acetaminophen on the functional activity of the liver, which was displayed in the decrease of activity of drug-induced cytolytic and cholestatic syndromes, normalizing the level of total bilirubin in rats’ serum. The appearance of the liver, its relative and absolute weight in animals with drug-induced hepatitis, who received LHT-8-16, was comparable with the intact rats. On histological specimens stained with hematoxylin and eosin we observed a decrease in the area of necrosis of hepatic parenchyma, preservation of tissue structure. Hyperemia and extending sinusoids occurred on the periphery of the body and were less pronounced in comparison with the control group. The area of the cytoplasm, the nuclei and the nuclear-cytoplasmic ratio approximated the values of the intact group. The appearance of multi-nuclear hepatocytes referred to the activation of the synthetic activity of the liver and onset of regeneration mechanisms. Thus, it can be concluded that the studied formulation possesses hepatoprotective property.

scholarly journals Pengaruh Pemberian Fraksi Etil Asetat Buah Kasturi (Mangifera casturi Kosterm.) Terhadap Gambaran Makroskopis-Mikroskopis Organ Hati Tikus Putih Jantan

2019 ◽  
Vol 6 (1) ◽  
pp. 106
Author(s):  
Sutomo Sutomo ◽  
Muhammad Rafi ◽  
Arnida Arnida
Keyword(s):  
Ethyl Acetate ◽  
Histopathological Examination ◽  
Ethyl Acetate Fraction ◽  
Control Group ◽  
Control Group Design ◽  
Hydropic Degeneration ◽  
Treatment Groups ◽  
Group Design ◽  
White Rats ◽  
Post Test

ABSTRAK             Kasturi (Mangifera casturi Kosterm) merupakan tumbuhan khas Kalimantan Selatan yang memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk menentukan gambaran makroskopis (warna dan volume) organ hati serta perubahan histopatologi sel hati (degenerasi parenkimatosa, degenerasi hidropis, dan nekrosis) pada tikus setelah diberikan fraksi etil asetat buah M. casturi secara peroral. Penelitian ini berupa penelitian eksperimental dengan rancangan post test only control group design. Hewan dibagi menjadi 5 kelompok yaitu kelompok kontrol (Na-CMC 0,5%) dan kelompok perlakuan (5, 50, 300, dan 2000 mg/kgBB). Setiap kelompok terdiri dari 5 tikus putih jantan yang diberikan perlakuan 1 kali selama 14 hari, setelah itu tikus dibedah dan diambil organ hati untuk diamati. Hasil analisis Kruskal Wallis menunjukkan bahwa pemberian fraksi etil asetat buah M. casturi pada gambaran makroskopis (volume) hati tidak berbeda bermakna antara kelompok kontrol dan perlakuan (p > 0,05). Hasil analisis One-Way ANOVA pada gambaran mikroskopis hati menunjukkan bahwa kelompok kontrol dan perlakuan tidak terdapat perbedaan bermakna. Kata kunci : Mangifera casturi, fraksi etil asetat, toksisitas, degenerasi, nekrosis  ABSTRACT Kasturi (Mangifera casturi Kosterm.) is the typical plants of South Kalimantan that contains antioxidant activity. The aim of this study was to determine the macroscopical representative (color and volume) of rats liver and the microscopical representative of hepar cells (parenchymatous degeneration, hydropic degeneration, and necrosis) after oral administration of ethyl acetate fraction of M. casturi fruit. This study was an experimental study with post-test only control group design. Test animals were divided into 5 groups consisted of a control group (CMC Na 0,5%), and treatment groups (5, 50, 300, and 2000 mg/kg bw). Each group consisted of 5 the male white rats which were treated once for 14 days. And then the rats were dissected, the liver were taken out to make preparations of histopathological examination and observation. The results of Kruskal Wallis showed that ethyl acetate fraction of M. casturi fruit had no difference on macroscopical representation of the liver. Keywords : Mangifera casturi, ethyl acetate fraction, toxicity, degeneration, necrosis

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