scholarly journals Inhibition of Nitric Oxide Synthase in Hyperdynamic Circulation of Rats with Early or Late Cirrhosis Secondary to Common Bile Duct Ligation

2011 ◽  
Vol 78 (3) ◽  
pp. 146-155 ◽  
Author(s):  
Yoshihito Kato ◽  
Yasumi Katsuta ◽  
Xue-Jun Zhang ◽  
Masaru Ohsuga ◽  
Toshio Akimoto ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bile Duct ◽  
Nitric Oxide Synthase ◽  
Common Bile Duct ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Hyperdynamic Circulation ◽  
Duct Ligation ◽  
Oxide Synthase

Effects of chronic nitric oxide activation or inhibition on early hepatic fibrosis in rats with bile duct ligation

1999 ◽  
Vol 96 (3) ◽  
pp. 297-305 ◽  
Author(s):  
Paula MAYORAL ◽  
Manuela CRIADO ◽  
Froilan HIDALGO ◽  
Olga FLORES ◽  
Miguel A. ARÉVALO ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bile Duct ◽  
Nitric Oxide Synthase ◽  
Hepatic Fibrosis ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Western Blots ◽  
Circulatory Effects ◽  
Duct Ligation ◽  
Oxide Synthase

Hepatic fibrosis or increased liver collagen contents drive functional abnormalities that, when extensive, may be life threatening. The purpose of this study was to assess the effects of the chronic stimulation or inhibition of nitric oxide synthesis in rats with hepatic fibrosis induced by permanent common bile duct ligation (3 weeks) and the role of expression of the different nitric oxide synthase isoforms. Bile duct ligation led to an important accumulation of collagen in the hepatic parenchyma, as shown both histologically and by the hydroxyproline contents of livers. Bilirubin and serum enzyme activities (measured as markers of cholestasis) increased several-fold after bile duct ligation. The area of fibrotic tissue, liver hydroxyproline content and serum markers of cholestasis were clearly related in obstructed rats. The absence of modifications in haemodynamic parameters excludes circulatory changes from being responsible for the development of liver alterations. In animals treated with NG-nitro-L-arginine methyl ester (L-NAME) the area of fibrosis was similar to that of untreated animals, the signs of cholestasis and cellular injury being more evident. In rats treated with L-arginine the area of fibrosis was almost three times larger than that found in bile duct ligated rats and in L-NAME-treated bile duct ligated rats, although the observed biochemical changes were similar to those seen in rats treated with L-NAME. Our results with inducible nitric oxide synthase, obtained by Western blots and immunohistochemistry, indicate a greater expression of the inducible enzyme in bile duct ligated and L-arginine-treated animals and a lower expression in the L-NAME and control groups. Constitutive nitric oxide synthase expression, obtained by Western blots, was very similar in all groups, except for the L-arginine-treated rats in which it was lower. These results suggest that nitric oxide production may be a key factor in the development of fibrosis in bile duct ligated rats. They also support the hypothesis of a dual role for nitric oxide; one beneficial, mediated by its circulatory effects, and the second negative, through its local toxic effects.

scholarly journals Pathophysiology of Lung Injury Induced by Common Bile Duct Ligation in Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94550 ◽  
Author(s):  
Fumiaki Shikata ◽  
Tomohisa Sakaue ◽  
Koh-ichi Nakashiro ◽  
Mikio Okazaki ◽  
Mie Kurata ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Lung Injury ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

scholarly journals A new model of reversible obstructive jaundice using rapidly absorbable suture materials

2012 ◽  
Vol 35 (6) ◽  
pp. 351 ◽  
Author(s):  
Nurettin Kahramansoy ◽  
Hayri Erkol ◽  
Edip E Yilmaz ◽  
Mustafa Şit ◽  
Fahri Yilmaz ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Obstructive Jaundice ◽  
Surgical Intervention ◽  
Bile Duct Ligation ◽  
Absorbable Suture ◽  
Suture Materials ◽  
Common Bile Duct Ligation ◽  
Duct Ligation ◽  
Morphologic Changes

Purpose: Reversible obstructive jaundice models have some limiting features, including the need for a second anaesthesia, re-laparotomy and surgical intervention after common bile duct ligation. The present study investigates the feasibility of a new application that can eliminate these limitations. Rapidly absorbable suture materials were used for ligation; therefore, spontaneous biliary decompression was anticipated by the self release of these rapidly degrading materials. Methods: Common bile ducts in Wistar Albino rats were ligated with silk, polyglytone 6211, or irradiated polyglactine 910 (n=7 for each group). Rats were grouped according to both the suture materials and the experiments termination date: 5 days (sham, silk5, polyglytone5, polyglactine5) and 21 days (silk21, polyglytone21, polyglactine21) after the ligation. Biochemical and morphologic changes of liver were assessed. Results: The group polyglactine21 showed significantly lower mean ALT, AST, GGT, total and direct bilirubin values when compared with the group polyglactine5 (p=0.004-0.037). Morphologic changes did not correlate with the biochemical amelioration. In the group polyglytone21, not only the biochemical but also the morphologic changes significantly ameliorated when compared with the group polyglytone5 (p=0.003-0.043). No procedure associated mortality was observed. Conclusion: Common bile duct ligation with polyglytone offers a new reversible model for prolonged obstructive jaundice which abolishes the need for relaparotomy and a second surgical intervention and significantly reduces mortality.

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