scholarly journals Bone Marrow Regeneration Using Adipose-Derived Stem Cells

2006 ◽  
Vol 73 (1) ◽  
pp. 45-47 ◽  
Author(s):  
Rei Ogawa ◽  
Hiroshi Mizuno ◽  
Hiko Hyakusoku ◽  
Takashi Shimada
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Derived Stem Cells ◽  
Bone Marrow Regeneration

Regeneration of Hematopoietic Microenvironment (Bone Marrow) Using Adipose-Derived Stem Cells - The 2nd Report.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4304-4304
Author(s):  
Rei Ogawa ◽  
Juri Fujimura ◽  
Hiroshi Mizuno ◽  
Hiko Hyakusoku ◽  
Takashi Shimada
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Transgenic Mice ◽  
Bone Matrix ◽  
Hematopoietic Cells ◽  
High Porosity ◽  
Adipose Derived Stem Cells ◽  
Bone Marrow Diseases ◽  
Bone Marrow Regeneration

Abstract Backgrounds: We have reported bone marrow regeneration using adipose-derived stem cells (ASCs) since 2003. In our study using mice, we showed that not only bone matrix but also bone marrow could be regenerated subcutaneously in vivo. Transplanted ASCs proliferated and differentiated into osteoblasts in micropores on the surface of scaffolds made of hydroxyapatites (HA). We have not yet obtained incontrovertible proof that the ASCs differentiated into hematopoietic cells. However, we could regenerate a subcutaneous microenvironment in which hematopoietic cells survived, which may lead to new treatments for fibrotic bone marrow diseases, such as idiopathic myelofibrosis and osteopetrosis. Purposes: In this report, we examined the optimal shapes of scaffolds for bone and bone marrow regeneration using ASCs. Methods: Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, ASCs were isolated from the inguinal fat pads of GFP transgenic mice. ASCs were cultured in vitro and after three passages were seeded into the small pores of scaffolds of various shapes made of HA. The ASC-containing scaffolds were then subsequently implanted into immunocompetent mice subcutaneously. Two months later, the scaffolds were extirpated for histological, immunohistochemical and flow cytometry analyses. Results: Histological examination showed that the pores were filled with bone matrix and that the bone marrow was composed of adipocytes, hematopoietic cells, and vasculatures. Bone marrow regeneration on small, thin scaffolds of high porosity was better than on other scaffolds. Immunohistochemical analysis confirmed that the GFP+ ASCs that had differentiated into osteoblasts were composed of bone matrix. Flow cytometry analyses showed that the bone marrow was composed of blood cells, including populations of monocytes, lymphocytes and granulocytes. Conclusions: Bone marrow engineering has great potential for hematopoietic disease therapy. The regeneration of the hematopoietic system using ASCs might be useful in the future for treating hematopoietic stem cell diseases such as leukemia as well as fibrotic bone marrow diseases. We plan to continue looking for suitable scaffolds and methods for bone and bone marrow regeneration using ASCs.

scholarly journals Morphological, molecular and functional differences of adult bone marrow- and adipose-derived stem cells isolated from rats of different ages

2012 ◽  
Vol 318 (16) ◽  
pp. 2034-2048 ◽  
Author(s):  
Cristina Mantovani ◽  
Stefania Raimondo ◽  
Maryam S. Haneef ◽  
Stefano Geuna ◽  
Giorgio Terenghi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Derived Stem Cells ◽  
Adult Bone Marrow ◽  
Functional Differences ◽  
Different Ages ◽  
Adult Bone

scholarly journals Inferior In Vivo Osteogenesis and Superior Angiogenesis of Human Adipose-Derived Stem Cells Compared with Bone Marrow-Derived Stem Cells Cultured in Xeno-Free Conditions

2018 ◽  
Vol 7 (3) ◽  
pp. 315-315 ◽  
Author(s):  
Meadhbh A. Brennan ◽  
Audrey Renaud ◽  
Fabien Guilloton ◽  
Miryam Mebarki ◽  
Valerie Trichet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Derived Stem Cells ◽  
Cells Cultured

scholarly journals Tracking intracavernously injected adipose-derived stem cells to bone marrow

2011 ◽  
Vol 23 (6) ◽  
pp. 268-275 ◽  
Author(s):  
G Lin ◽  
X Qiu ◽  
T Fandel ◽  
L Banie ◽  
G Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Derived Stem Cells

Comparison of Human Adipose-Derived Stem cells and Bone Marrow-Derived Stem cells in a Myocardial Infarction Model

2014 ◽  
Vol 23 (2) ◽  
pp. 195-206 ◽  
Author(s):  
Jeppe Grøndahl Rasmussen ◽  
Ole Frøbert ◽  
Claus Holst-Hansen ◽  
Jens Kastrup ◽  
Ulrik Baandrup ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Derived Stem Cells

scholarly journals Expression of surface markers and myogenic potential of rat bone marrow- and adipose-derived stem cells: a comparative study

2013 ◽  
Vol 46 (2) ◽  
pp. 113 ◽  
Author(s):  
Vahid Bayati ◽  
Mahmoud Hashemitabar ◽  
Roohollah Gazor ◽  
Reza Nejatbakhsh ◽  
Dariush Bijannejad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Comparative Study ◽  
Surface Markers ◽  
Adipose Derived Stem Cells ◽  
Myogenic Potential ◽  
Rat Bone

Metabolic Analysis of Bone Marrow Mesenchymal Stem Cells and Adipose-Derived Stem Cells Using NMR-Based Metabolomics.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4244-4244
Author(s):  
Rei Ogawa ◽  
Juri Fujimura ◽  
Keiko Hirakawa ◽  
Kyoko Uekusa ◽  
Kohtaro Yuta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Computer Software ◽  
Principal Component ◽  
Adipose Derived Stem Cells ◽  
Detection Analysis ◽  
Marrow Mesenchymal Stem Cells ◽  
Metabolic Activities ◽  
And Control

Abstract Background: Nuclear magnetic resonance (NMR) -based metabolomics has been suggested to be useful for exhaustive analysis of metabolic pathways of cells or tissues. We report our study on bone marrow mesenchymal stem cells (BSCs) and adipose-derived stem cells (ASCs) using NMR-based metabolomics. Materials and Methods: Five-week-old Wister rats were used for this study. BSCs were harvested from the femur, ASCs from inguinal fat pads, and control fibroblasts from the abdominal dermis. The cells were cultured in DMEM with 10% fetal bovine serum and harvested after two passages of the subculture. Then, the cells were subjected to freezing in liquid nitrogen and crushed to extract the aqueous metabolites. 1H-NMR spectra was measured and analyzed by a computer software (Alice2 for metabolome™ and ADMEWORKS/ModelbuilderTM). Results: BSCs, ASCs and fibroblasts were clearly separated into three groups on a principal component analysis (PCA) plot. Conclusion: ASCs, BSCs and fibroblasts were considered to have different metabolic activities, and NMR-based metabolomics will henceforth be useful for the detection, analysis, and characterization of various kinds of stem cells.

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