scholarly journals Purification and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans.

1991 ◽  
Vol 55 (8) ◽  
pp. 2091-2097 ◽  
Author(s):  
Tsuyoshi Sucio ◽  
Hiroyuki SUZUKI ◽  
Aya OTO ◽  
Kenji INAGAKI ◽  
Hidehiko TANAKA ◽  
...  
1991 ◽  
Vol 55 (8) ◽  
pp. 2091-2097 ◽  
Author(s):  
Tsuyoshi Sugio ◽  
Hiroyuki Suzuki ◽  
Aya Oto ◽  
Kenji Inagaki ◽  
Hidehiko Tanaka ◽  
...  

1985 ◽  
Vol 49 (6) ◽  
pp. 1401-1406 ◽  
Author(s):  
Tsuyoshi Sugio ◽  
Chitoshi Domatsu ◽  
Osamu Munakata ◽  
Tatsuo Tano ◽  
Kazutami Imai

1966 ◽  
Vol 12 (5) ◽  
pp. 957-964 ◽  
Author(s):  
J. Landesman ◽  
D. W. Duncan ◽  
C. C. Walden

Oxidation of various inorganic sulfur compounds by Thiobacillus ferrooxidans was studied, and conditions necessary for maximum respiration rates were established. Optimum oxidation of elemental sulfur occurred at pH 5.0 and gave a Qo2(N) of 726; oxidation of thiosulfate gave a maximum Qo2(N) of 514 at pH 4.0; tetra- and tri-thionate, when oxidized at pH 6.0, gave a maximum Qo2(N) of 103 and 113, respectively. Polythionates accumulated during thiosulfate oxidation, but did not during oxidation of elemental sulfur. Metallic sulfide minerals were oxidized optimally as follows: chalcopyrite, pH 2.0, maximum Qo2(N) 3200; bornite, pH 3.0, maximum Qo2(N) 450; pyrite, pH 2.0, maximum Qo2(N) 1600. Maximum temperature for oxidation of all inorganic sulfur compounds tested was 40 C.The effect of a variety of organic compounds on sulfur oxidation is presented.T. ferrooxidans requires growth adaptation on iron for maximum respiration on that substrate; however, sulfur oxidation is not inducible. Iron and sulfur can be oxidized simultaneously, giving a rate equal to the sum of the maximum rates of oxidation of the two substrates individually.


Author(s):  
Hong Liu ◽  
Xun Liu ◽  
Ning Ding

Microelectrodes can be used to obtain chemical profiles within biofilm microenvironments. For example, sulfate (SO42−) and hydrogen sulfide (H2S) microelectrodes can be used to study sulfate reduction activity in this context. However, there is no SO42− microelectrode available for studying sulfate reduction in biofilms. In this study, SO42− and H2S microelectrodes were fabricated and applied in the measurement of a wastewater membrane-aerated biofilm (MAB) to investigate the in situ sulfate reduction activity. Both the SO42− and H2S microelectrodes with a tip diameter of around 20 micrometers were successfully developed and displayed satisfying selectivity to SO42− and H2S, respectively. The Nernstian slopes of calibration curves of the fabricated SO42− electrodes were close to −28.1 mV/decade, and the R2 values were greater than 98%. Within the selected concentration range from 10−5 M (0.96 mg/L) to 10−2 M (960 mg/L), the response of the SO42− microelectrode was log-linearly related to its concentration. The successfully fabricated SO42− microelectrode was combined with the existing H2S microelectrode and applied on an environmental wastewater biofilm sample to investigate the sulfate reduction activity within it. The H2S and SO42− microelectrodes showed stable responses and good performance, and the decrease of SO42− with an accompanying increased of H2S within the biofilm indicated the in situ sulfate reduction activity. The application of combined SO42− and H2S microelectrodes in wastewater biofilms could amend the current understanding of sulfate reduction and sulfur oxidation within environmental biofilms based on only H2S microelectrodes.


1986 ◽  
Vol 50 (11) ◽  
pp. 2755-2761 ◽  
Author(s):  
Tsuyoshi SUGIO ◽  
Wataru MIZUNASHI ◽  
Tatsuo TANO ◽  
Kazutami IMAI

1996 ◽  
Vol 178 (19) ◽  
pp. 5776-5780 ◽  
Author(s):  
N Ohmura ◽  
K Tsugita ◽  
J I Koizumi ◽  
H Saika

1986 ◽  
Vol 52 (6) ◽  
pp. 507-518 ◽  
Author(s):  
W. Hazeu ◽  
W. Bijleveld ◽  
J. T. C. Grotenhuis ◽  
E. Kakes ◽  
J. G. Kuenen

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