scholarly journals Structures of integrated DNA containing human adenovirus E1A gene in transgenic mice.

1988 ◽  
Vol 52 (10) ◽  
pp. 2537-2546 ◽  
Author(s):  
Takashi NINOMIYA ◽  
Masaki HOSHI ◽  
Atsushi YUKI
Keyword(s):  
Transgenic Mice ◽  
Human Adenovirus ◽  
Adenovirus E1a ◽  
E1a Gene

Structures of Integrated DNA Containing Human Adenovirus E1A Gene in Transgenic Mice

1988 ◽  
Vol 52 (10) ◽  
pp. 2537-2546
Author(s):  
Takashi Ninomiya ◽  
Masaki Hoshi ◽  
Atsushi Yuki
Keyword(s):  
Transgenic Mice ◽  
Human Adenovirus ◽  
Adenovirus E1a ◽  
E1a Gene

Transcription of the human adenovirus E1a gene in Saccharomyces cerevisiae

Gene ◽  
1985 ◽  
Vol 33 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Handa Hiroshi ◽  
Mizumoto Kiyohisa ◽  
Oda Kinichiro ◽  
Okamoto Takashi ◽  
Fukasawa Toshio
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Human Adenovirus ◽  
Adenovirus E1a ◽  
E1a Gene

scholarly journals Introduction of human adenovirus E1A gene into fertilized mouse eggs.

1987 ◽  
Vol 33 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Masaki HOSHI ◽  
Takashi NINOMIYA ◽  
Yasunari SAITO ◽  
Atsushi YUKI
Keyword(s):  
Human Adenovirus ◽  
Adenovirus E1a ◽  
E1a Gene ◽  
Mouse Eggs

Disruption of the coordinate expression of muscle genes in a transfected BC3H1 myoblast cell line producing a low level of the adenovirus E1A transforming protein

1992 ◽  
Vol 70 (10-11) ◽  
pp. 1268-1276 ◽  
Author(s):  
Joe S. Mymryk ◽  
Janice D. Oakes ◽  
Senthil K. Muthuswamy ◽  
Pietro D'Amico ◽  
Stanley T. Bayley ◽  
...  
Keyword(s):  
Myogenic Differentiation ◽  
Calf Serum ◽  
Adenovirus E1a ◽  
Myoblast Cell Line ◽  
Muscle Genes ◽  
E1a Gene ◽  
H1 Cells ◽  
Myoblast Cell ◽  
Muscle Gene ◽  
Adenovirus 5

Mouse BC3H1 myoblasts were stably transfected with the adenovirus 5 E1A gene. One clonal line, BC3E7, was found to differ in some important respects from those previously reported for E1A-transformed myoblasts. In contrast to BC3H1 cells which differentiate when confluent in medium containing 0.5% fetal calf serum (FCS), BC3E7 cells failed to elongate and align, to express acetylcholine receptor and creatine kinase, and to down-regulate expression of β- and γ-actins and tropomyosin isoform (TM) 1. However, increased synthesis of TMs 2, 3, and 4, and myosin light chain 1 associated with differentiation in BC3H1 still occurred in BC3E7 cells, and most surprisingly, α-actin was produced at a significant level in both proliferating and confluent BC3E7 cells. Interestingly, myogenin was expressed in confluent BC3E7 cells in 0.5% FCS, but not in 20%. The level of E1A expression in BC3E7 cells was found to be very low by analysis of mRNA, by immunoprecipitation of E1A protein, and by the ability of BC3E7 cells to complement the E1A-deficient adenovirus mutant dl312. These results suggest that different levels of E1A may be needed to repress different promoters and that E1A does not block myogenic differentiation by repressing myogenin expression, but represses each muscle gene independently.Key words: actin, adenovirus 5 E1A, BC3H1 myoblasts, myogenin.

scholarly journals Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells.

1988 ◽  
Vol 8 (11) ◽  
pp. 4799-4807 ◽  
Author(s):  
L J Brunet ◽  
A J Berk
Keyword(s):  
Adenovirus Type ◽  
In Vitro Transcription ◽  
Mrna Levels ◽  
Adenovirus E1a ◽  
Transcription System ◽  
Nuclear Extracts ◽  
Tumor Virus ◽  
E1a Gene ◽  
High Level

The adenovirus E1A proteins are essential for the normal temporal activation of transcription from every other adenoviral early promoter. High-level E1A expression in the absence of viral infection would facilitate biochemical studies of E1A-mediated transactivation. Toward this end, we introduced the adenovirus type 2 E1A gene under the control of the murine mammary tumor virus promoter into HeLa cells. Uninduced cells expressed little or no detectable E1A mRNA. Upon induction, mRNA levels accumulated to about 50% of the level observed in 293 cells. The level of E1A expression in these cells could be controlled by varying the concentration of the inducing glucocorticoid. Under these conditions of varying E1A concentrations, it was observed that activation of the E2, E3, and E4 promoters of H5dl312 initiated at the same E1A concentration and that transcription from each promoter increased as the E1A concentration increased. These results indicate that E1A-mediated transactivation is proportional to the concentration of E1A protein. E1A-dependent transcriptional stimulation of the E4 promoter was reproduced in an in vitro transcription system, demonstrating that expression of only the E1A proteins was sufficient to increase the transcriptional activity of nuclear extracts.

Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells

1988 ◽  
Vol 8 (11) ◽  
pp. 4799-4807
Author(s):  
L J Brunet ◽  
A J Berk
Keyword(s):  
Adenovirus Type ◽  
In Vitro Transcription ◽  
Mrna Levels ◽  
Adenovirus E1a ◽  
Transcription System ◽  
Nuclear Extracts ◽  
Tumor Virus ◽  
E1a Gene ◽  
High Level

The adenovirus E1A proteins are essential for the normal temporal activation of transcription from every other adenoviral early promoter. High-level E1A expression in the absence of viral infection would facilitate biochemical studies of E1A-mediated transactivation. Toward this end, we introduced the adenovirus type 2 E1A gene under the control of the murine mammary tumor virus promoter into HeLa cells. Uninduced cells expressed little or no detectable E1A mRNA. Upon induction, mRNA levels accumulated to about 50% of the level observed in 293 cells. The level of E1A expression in these cells could be controlled by varying the concentration of the inducing glucocorticoid. Under these conditions of varying E1A concentrations, it was observed that activation of the E2, E3, and E4 promoters of H5dl312 initiated at the same E1A concentration and that transcription from each promoter increased as the E1A concentration increased. These results indicate that E1A-mediated transactivation is proportional to the concentration of E1A protein. E1A-dependent transcriptional stimulation of the E4 promoter was reproduced in an in vitro transcription system, demonstrating that expression of only the E1A proteins was sufficient to increase the transcriptional activity of nuclear extracts.

scholarly journals Induction of cell cycle progression by adenovirus E1A gene 13S- and 12S-mRNA products in quiescent rat cells.

1987 ◽  
Vol 7 (10) ◽  
pp. 3846-3852 ◽  
Author(s):  
T Nakajima ◽  
M Masuda-Murata ◽  
E Hara ◽  
K Oda
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Cell Cycle Progression ◽  
Adenovirus Type ◽  
Inducible Promoter ◽  
Similar Rate ◽  
Cycle Progression ◽  
Adenovirus E1a ◽  
Tumor Virus ◽  
E1a Gene

Rat 3Y1 cell lines that express either adenovirus type 12 E1A 13S mRNA or 12S mRNA in response to dexamethasone treatment were established by introduction of recombinant vector DNA containing the E1A 13S- or 12S-mRNA cDNA placed downstream of the hormone-inducible promoter of mouse mammary tumor virus. These cell lines were growth arrested, and the induction of cell cycle progression was analyzed by flow cytometry after switch on of the cDNA by the addition of dexamethasone. The results indicate that the 13S- or 12S-mRNA product alone has the ability to cause progression of the cell cycle at a similar rate. The simultaneous addition of epidermal growth factor accelerated the rate of cell cycle progression in the transition from the G0/G1 phase to the S phase.

scholarly journals Comparative Sequence Analysis of the Largest E1A Proteins of Human and Simian Adenoviruses

2002 ◽  
Vol 76 (16) ◽  
pp. 7968-7975 ◽  
Author(s):  
Nikita Avvakumov ◽  
Russ Wheeler ◽  
Jean Claude D'Halluin ◽  
Joe S. Mymryk
Keyword(s):  
Gene Expression ◽  
Human Adenovirus ◽  
Detailed Comparison ◽  
Adenovirus Type ◽  
Comparative Sequence Analysis ◽  
Regulatory Proteins ◽  
Carboxyl Terminus ◽  
Adenovirus E1a ◽  
Simian Adenovirus ◽  
Adenovirus Type 5

ABSTRACT The early region 1A (E1A) gene is the first gene expressed after infection with adenovirus and has been most extensively characterized in human adenovirus type 5 (hAd5). The E1A proteins interact with numerous cellular regulatory proteins, influencing a variety of transcriptional and cell cycle events. For this reason, these multifunctional proteins have been useful as tools for dissecting pathways regulating cell growth and gene expression. Despite the large number of studies using hAd5 E1A, relatively little is known about the function of the E1A proteins of other adenoviruses. In 1985, a comparison of E1A sequences from three human and one simian adenovirus identified three regions with higher overall levels of sequence conservation designated conserved regions (CR) 1, 2, and 3. As expected, these regions are critical for a variety of E1A functions. Since that time, the sequences of several other human and simian adenovirus E1A proteins have been determined. Using these, and two additional sequences that we determined, we report here a detailed comparison of the sequences of 15 E1A proteins representing each of the six hAd subgroups and several simian adenoviruses. These analyses refine the positioning of CR1, 2, and 3; define a fourth CR located near the carboxyl terminus of E1A; and suggest several new functions for E1A.

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