scholarly journals Properties of the agarase from a luminous bacterium, vibrio harveyi.

1987 ◽  
Vol 51 (1) ◽  
pp. 269-270 ◽  
Author(s):  
Shigeki FUKASAWA ◽  
Hidesaburo KOBAYASHI
Keyword(s):  
Vibrio Harveyi ◽  
Luminous Bacterium

scholarly journals Properties of the proteinase from a luminous bacterium, Vibrio harveyi strain FLN-77.

1989 ◽  
Vol 37 (1) ◽  
pp. 204-205 ◽  
Author(s):  
Shigeki FUKASAWA ◽  
Masaru MIYAHARA ◽  
Masayo HOSODA ◽  
Munetsugu KURATA
Keyword(s):  
Vibrio Harveyi ◽  
Luminous Bacterium

scholarly journals Properties of the Agarase from a Luminous Bacterium,Vibrio harveyi

1987 ◽  
Vol 51 (1) ◽  
pp. 269-270
Author(s):  
Shigeki Fukasawa ◽  
Hidesaburo Kobayashi
Keyword(s):  
Vibrio Harveyi ◽  
Luminous Bacterium

scholarly journals Immunogold labeling of luciferase in the luminous bacterium Vibrio harveyi after fast-freeze fixation and different freeze-substitution and embedding procedures.

1989 ◽  
Vol 37 (5) ◽  
pp. 663-674 ◽  
Author(s):  
M T Nicolas ◽  
J M Bassot ◽  
G Nicolas
Keyword(s):  
Vibrio Harveyi ◽  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Immunogold Labeling ◽  
Chemical Fixation ◽  
Luminous Bacterium ◽  
Immunogold Staining ◽  
Bioluminescent Bacterium ◽  
Lr White ◽  
Freeze Fixation

We studied the ultrastructural localization of luciferase on sections of the bioluminescent bacterium Vibrio harveyi by indirect immunogold staining, using a polyclonal antiluciferase antibody and the usual control tests, after chemical fixation or fast-freeze fixation (FFF) followed by different freeze-substitution (FS) procedures and embedding in either Epon or LR White. After liquid fixation with glutaraldehyde and paraformaldehyde and LR White embedding, labeling occurred over the cytoplasm but not over the condensed nucleoid. Epon embedding almost abolished it. FFF-FS considerably improved the morphological preservation and revealed cytoplasmic "patches" with a complex ultrastructure in Epon sections. The preservation was always less good in LR White. The patches were densely labeled, even in Epon sections, after FS in acetone. However, labeling intensity was 3.7 times greater in LR White than in Epon. With both resins, labeling diminished similarly when fixative agents were present in the FS medium. The localization of luciferase in the cytoplasm and particularly in the patches is discussed.

scholarly journals Purification and properties of a proteinase from a marine luminous bacterium, Vibrio harveyi strain FLA-11.

1988 ◽  
Vol 52 (2) ◽  
pp. 435-441 ◽  
Author(s):  
Shigeki FUKASAWA ◽  
Kenji NAKAMURA ◽  
Atsushi KAMII ◽  
Yoshitaka OHYAMA ◽  
Masako OSUMI
Keyword(s):  
Vibrio Harveyi ◽  
Luminous Bacterium ◽  
Marine Luminous Bacterium ◽  
Purification And Properties

scholarly journals Some properties of two proteinases from a luminous bacterium, Vibrio harveyi strain FLN-108.

1988 ◽  
Vol 52 (12) ◽  
pp. 3009-3014 ◽  
Author(s):  
Shigeki FUKASAWA ◽  
Kenji NAKAMURA ◽  
Masaru MIYAHIRA ◽  
Munetsugu KURATA
Keyword(s):  
Vibrio Harveyi ◽  
Luminous Bacterium

Effects of abiotic and biotic factors on Vibrio harveyi ATCC 14126T survival dynamics in seawater microcosms

2019 ◽  
Vol 83 (2) ◽  
pp. 109-118 ◽  
Author(s):  
M Orruño ◽  
C Parada ◽  
E Ogayar ◽  
VR Kaberdin ◽  
I Arana
Keyword(s):  
Vibrio Harveyi ◽  
Biotic Factors ◽  
Abiotic And Biotic Factors

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

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