scholarly journals Effect of hydroxylamine on formation of phospholipid molecular species containing cyclopropane fatty acid in Escherichi coli.

1985 ◽  
Vol 49 (3) ◽  
pp. 875-876
Author(s):  
Masataka ISHINAGA ◽  
Eiko FUJISHIGE ◽  
Akiko KAKIMOTO
Keyword(s):  
Fatty Acid ◽  
Molecular Species ◽  
Cyclopropane Fatty Acid ◽  
Phospholipid Molecular Species ◽  
Escherichi Coli

scholarly journals INVESTIGATION ON CONTENT OF FATTY ACIDS, PHOSPHOLIPIDS, AND PHOSPHOLIPID MOLECULAR SPECIES COMPOSITION OF THE VIETNAMESE CORAL Bebryce sp.

2018 ◽  
Vol 18 (2) ◽  
pp. 178-186
Author(s):  
Dang Thi Phuong Ly ◽  
Pham Minh Quan ◽  
Trinh Thi Thu Huong ◽  
Valeria P. Grigorchuk ◽  
Pham Quoc Long ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Molecular Species ◽  
Soft Coral ◽  
Unsaturated Fatty Acids ◽  
Total Phospholipid ◽  
Saturated Fatty Acids ◽  
Phospholipid Content ◽  
Fatty Acids Composition ◽  
Phospholipid Molecular Species

In the fatty acid composition of total lipid of the soft coral Bebryce sp., the concentration of unsaturated fatty acid predominates. The composition of saturated fatty acids is very diverse, including all saturated fatty acids from C14 to C26. The unsaturated fatty acids with high concentration are C20: 4n-6, 20:5n-3, 22:6n-3, 24:5n-6, 26:3n-6, 26:2n-6, 26:2n-7, 28:3n-6. In the fatty acids composition of the studied coral, there is presence of characterized fatty acids for the existence of sponges C25-C28 (demospongic fatty acids) with total content 29,86%. Most of the Bebryce coral species do not have zooxanthellae, therefore, in the fatty acids composition, either it is lack or contains only a small amount of markers fatty acids for zooxanthellae such as 18:5n-3, 18:4n-3, 18:3n-6, 20:4n-3. In the phospholipid content of the soft coral Bebryce sp., there is presence of characterized classes for Cnidarian animals such as phosphatidylethanolamine (PE), phosphatidylchonline (PC), phosphatidylserine (PS), phosphatidylinositol (PI), phosphonolipid is ceramide aminoethylphosphonate (CAEP). PC account for the highest concentration (37,20% of total phospholipid). The molecular species of phospholipid classes of Bebryce sp. for the first time were investigated. In the results, there we 60 phospholipid molecular species identified in 5 classes. The molecular species with high content in the classes were PE 20:4/18:1e, PE 20:4/19:1, PC 20:4/18:0e, PC 20:4/16:0e, PS 24:5/18:0e, PI 24:5/18:0, CAEP18:2base/16:0 and CAEP 18:1base/16:0.

Replacement of molecular species of phosphatidylcholine: influence on erythrocyte Na transport

1990 ◽  
Vol 258 (4) ◽  
pp. C682-C691 ◽  
Author(s):  
B. Engelmann ◽  
J. A. Op den Kamp ◽  
B. Roelofsen
Keyword(s):  
Exchange Rate ◽  
Fatty Acid ◽  
Molecular Species ◽  
Bovine Liver ◽  
Prolonged Incubation ◽  
Phospholipid Molecular Species ◽  
Transfer Protein ◽  
Pump Rate ◽  
Large Populations ◽  
Selective Incorporation

The phosphatidylcholine-specific transfer protein (PC-Tp) from bovine liver was used to replace endogeneous erythrocyte phosphatidylcholine (PC) with various amounts of five different molecular species of PC. Furosemide-sensitive (FS) Rb uptake, Na-Li exchange, and Na-K pump rates were considered in relation to the nature and extent of those replacements. Changes in fatty acid contents of PC after incorporation of different molecular species fell within a variation range (10-30%) similar to that found in large populations of healthy individuals. Di16:0-PC accelerated Na-Li exchange and FS Rb uptake by approximately 40 and 25%, respectively. Some reduction (20%) in FS Rb uptake was seen in 16:0/18:2-PC-enriched erythrocytes. Incorporation of 16:0/22:6-PC accelerated Na-Li exchange and FS Rb uptake by greater than 40 and 20%, respectively. Apart from inhibitory effects of 16:0/18:1-PC and di16:0-PC (24 and 19%, respectively) the Na-K pump rate was virtually unchanged by incorporation of different PC molecular species. Exogeneous PC molecules are exclusively inserted in the outer membrane leaflet and, particularly in the case of di16:0-PC, migrate slowly to the cytoplasmic leaflet. Prolonged incubation of cells (up to 21 h) after replacement with di16:0-PC showed that both Na-Li exchange and FS Rb uptake rates responded differently to redistribution of newly inserted molecules over both bilayer halves. Compared with cells exhibiting a selective incorporation of di16:0-PC in the outer monolayer, additional enrichment with disaturated species in the inner monolayer accelerated FS Rb uptake, whereas Na-Li exchange rate reverted to control values. It is concluded that small changes in fatty acid composition of PC induced by limited replacement of phospholipid molecular species can cause considerable changes in Na-Li exchange rate and FS Rb uptake. Differences in phospholipid molecular species composition could contribute to known interindividual variability of both Na-Li exchange and Na-K cotransport.

Multiple Erythroid Isoforms of Human Long-Chain acyl-CoA Synthetases Are Produced by a Switch of the Fatty Acid Gate-Domains.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1672-1672
Author(s):  
Eric Soupene ◽  
Frans A. Kuypers
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Substrate Specificity ◽  
Species Composition ◽  
Molecular Species ◽  
Molecular Species Composition ◽  
Phospholipid Molecular Species ◽  
Chain Acyl ◽  
Long Chain ◽  
Plasma Membrane Phospholipid

Abstract In mammals, long-chain acyl-CoA synthetases (ACSL) are necessary for fatty acid degradation, phospholipid remodeling, and production of long acyl-CoA esters that regulate various physiological processes. These enzymes play a crucial role in plasma membrane phospholipid turnover in erythrocytes, maintaining the complex phospholipid molecular species composition essential for proper membrane function. The mechanism by which this highly dynamic turnover together with an ever-changing plasma fatty acid pool maintains phospholipid composition is poorly understood. We have previously cloned Acyl-CoA Synthetase Long-chain member 6 (ACSL6), the isoform responsible for activation of long-chain fatty acids in erythrocytes. Two additional transcript variants of this protein were subsequently isolated from brain and testis. We report the expression of four different variants of ACLS6 in reticulocytes, one as we originally reported, two of which are novel, one as was identified in brain cells. PCR amplifications using primers for the predicted variable regions were performed from cDNAs of CD34 positive erythroid progenitors, K562 cells, fetal blood cells, reticulocytes and placenta. ACSL variants were expressed in E. coli host BL21DE3 cells using the pET28a vector, and detected by His tag immuno detection. Sequence alignments were generated using sequences retrieved from RefSeq and GenBank databases on the NCBI site. Exon and intron definition for ACSL members were obtained using evidence viewer and model maker available at the map viewer page of each gene. We identified four different spliced variants of ACSL6 in erythroid cells based on a mutually exclusive exon pair. Each exon of this pair encodes a slightly different short motif that contains the fatty acid Gate domain, a conserved structural domain found in all vertebrate and invertebrate ACSL homologs. The motif differs in the presence of either the aromatic residue phenylalanine (Phe) or tyrosine (Tyr), and seems to play a role in substrate specificity. One of the new forms contained an exon not found in any other ACSL isoforms. Erythroid precursors also express the closely related ACSL1, and we characterized two additional isoforms of this protein, similar to ACSL6. When analyzed on denaturing SDS polyacrylamide gel both ACSL1 and 6 appeared to exist as a dimer. Based on our results, we propose the generation of two different Gate-domains by alternative splicing of the two exons in these proteins. One represents a switch of the Phe to the Tyr Gate-domain motif, the other resulted of the exclusion of both. Swapping of this motif appears to be common to all mammalian homologs of ACSL1 and 6. We conclude that the Phe to a Tyr substitution in the Gate-domain, or its removal, together with the formation of homo or heterodimers will allow ACSL6 the structural diversity to define substrate specificity that maintains the complex plasma membrane phospholipid molecular species composition in erythrocytes.

scholarly journals Comprehensive study of the lipid from whelk (Chlorostoma rusticum and Neverita didyma) with emphasis on characterization of phospholipid molecular species by shot-gun strategy

2019 ◽  
Vol 8 ◽  
Author(s):  
Zhong-Yuan Liu ◽  
Kai-Qi Gang ◽  
Fa-Wen Yin ◽  
Hong-Kai Xie ◽  
Liang Song ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Species ◽  
Chain Polyunsaturated Fatty Acid ◽  
Total Lipids ◽  
Phospholipid Molecular Species ◽  
Promising Source ◽  
Long Chain ◽  
Fatty Acid Compositions ◽  
Comprehensive Study

In the present study, an effective shot-gun lipidomic methodology was established to determine the glycerophospholipid (GP) molecular species of two species of edible marine whelks (Chlorostoma rusticum and Neverita didyma). Simultaneously, the lipid content, lipid classes, phospholipid (PL) subclasses and fatty acid compositions were also investigated. Over 210 molecular species of GP including glycerophosphocholine, lysoglycerophosphocholine, glycerophosphoethanolamine, lysoglycerophosphoethanolamine, glycerophosphoserine, lysoglycerophosphoserine, glycerophosphoinositol and lysoglycerophosphoinositol were characterized in the two abovementioned whelk species. The predominant GP molecular species contained n-3 long chain polyunsaturated fatty acid (n-3 LC-PUFA), especially docosahexaenoic acid and eicosapentaenoic acid. Meanwhile, PL (57.70-58.86% of total lipids) and PUFA (21.69-37.68% of total FA) take large proportions in whelk lipids. Among PL, phosphatidylcholine (50.58-52.41 mol%) and phosphatidylethanolamine (27.67-32.73 mol%) were dominant. Therefore, marine whelks turn out to be promising source of n-3 LC-PUFA existed in PL form and thus directly contribute to the health benefits of consumer.

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