scholarly journals Selection of high ubiquinone 10-producing strain of tobacco cultured cells by cell cloning technique.

1980 ◽  
Vol 44 (4) ◽  
pp. 967-969 ◽  
Author(s):  
Takashi MATSUMOTO ◽  
Tsutomu IKEDA ◽  
Naomi KANNO ◽  
Takuro KISAKI ◽  
Masao NOGUCHI
Keyword(s):  
Cultured Cells ◽  
Cell Cloning ◽  
Cloning Technique ◽  
Ubiquinone 10 ◽  
Selection Of

Selection of High Ubiquinone 10-Producing Strain of Tobacco Cultured Cells by Cell Cloning Technique

1980 ◽  
Vol 44 (4) ◽  
pp. 967-969
Author(s):  
Takashi Matsumoto ◽  
Tsutomu Ikeda ◽  
Naomi Kanno ◽  
Takuro Kisaki ◽  
Masao Noguchi
Keyword(s):  
Cultured Cells ◽  
Cell Cloning ◽  
Cloning Technique ◽  
Ubiquinone 10 ◽  
Selection Of

scholarly journals Selection of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone 10 by a Cell Cloning Technique

1981 ◽  
Vol 45 (7) ◽  
pp. 1627-1633 ◽  
Author(s):  
Takashi Matsumoto ◽  
Naomi Kanno ◽  
Tsutomu Ikeda ◽  
Yukiteru Obi ◽  
Takuro Kisaki ◽  
...  
Keyword(s):  
Tobacco Cell ◽  
Cell Cloning ◽  
Cloning Technique ◽  
A Cell ◽  
Cell Strains ◽  
Ubiquinone 10 ◽  
Selection Of

Characteristics of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone-10 Selected by a Cell Cloning Technique

1981 ◽  
Vol 45 (10) ◽  
pp. 2259-2263 ◽  
Author(s):  
Tsutomu Ikeda ◽  
Takashi Matsumoto ◽  
Yukiteru Obi ◽  
Takuro Kisaki ◽  
Masao Noguchi
Keyword(s):  
Tobacco Cell ◽  
Cell Cloning ◽  
Cloning Technique ◽  
A Cell ◽  
Cell Strains ◽  
Ubiquinone 10

Phenolics from Cell Suspension Cultures of Penstemon serrulatus; Relation to Plant Organs

1997 ◽  
Vol 52 (7-8) ◽  
pp. 426-432 ◽  
Author(s):  
Zuzanna Skrzypek ◽  
Halina Wysokińska
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Naphthaleneacetic Acid ◽  
Phenolic Composition ◽  
Phenylpropanoid Glycosides ◽  
Selection Of

Abstract By repeated selection of pigment portions of tissue the red callus induced from root seed­lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/l), 6-benzylaminopurine (2 mg/l) and sucrose (50 g/l). From the cultured cells eight phenolic compounds were isolated. They were identified as cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, luteolin, luteolin 7-O-glucoside, norartocarpetin 7-O-glucoside, verbascoside, martynoside and leucosceptoside A. The kind of cell line, its age and light irradiation were important factors in flavonoid production, but production of phenylpropanoid glycosides was found to be unaffected by these factors. The phenolic composition found in the cell culture was compared with those in the flowers and leaves of original plants of P. serrulatus.

Plasminogen Activator (PA) Activity In Metastatic Cells: An Amidolytic Approach

1981 ◽  
Author(s):  
D Coen ◽  
A Bini ◽  
G Balconi ◽  
F Delaini ◽  
L Mussoni ◽  
...  
Keyword(s):  
Primary Tumor ◽  
Fibrinolytic Activity ◽  
Tumor Tissue ◽  
Cultured Cells ◽  
Cell Extracts ◽  
Mechanical Disruption ◽  
Metastatic Cells ◽  
Triton X ◽  
Selection Of

It has been proposed that fibrinolytic activity can play an important role in the process of metastasis formation. Nevertheless, it is not yet clear in which phase of the tumor growth and dissemination this activity is involved. We measured the fibrinolytic activity of cells from primary tumor and metastatic nodules of 3LL, an i.m. implanted murine tumor which selectively metastasizes to the lungs. Tumor cells have been studied both immediately after mechanical disruption of tumor tissue and after in vitro culturing to confluence. Their P.A. activity was tested by an amidolytic assay in which cells were incubated with purified plasminogen (3CU/ml) and 4mM S-2251 (Kabi Diagnostica, Stockholm, Sweden), a plasmin specific chromogenic substrate. After 3 hour incubation at 37°C, the reaction was stopped with acetic acid and absorbance read at 405 nm.Cells from the primary tumor and metastatic nodules showed a similar fibrinolytic activity, which was in both cases in- increased 3 to 4 fold in cell extracts obtained after preincubation with TRITON X-100. A dose-response curve plotted with increasing urokinase concentrations showed a parallel course. This data suggests that, in the 3LL model, PA activity is not one of the properties characterizing the selection of metastatic cells.On the other hand,cultured cells presented consistently higher levels of PA than their native counterparts, suggesting that adhesion of cells in culture may stimulate PA production or, alternatively, that cultured cells are a selected population in comparison to the overall number of native cells.

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