scholarly journals Isolation of intact chloroplasts from spinach leaf by centrifugation in gradients of the modified silica "Percoll".

1979 ◽  
Vol 43 (10) ◽  
pp. 2137-2142 ◽  
Author(s):  
Tetsuko TAKABE ◽  
Mikio NISHIMURA ◽  
Takashi AKAZAWA
Keyword(s):  
Modified Silica ◽  
Intact Chloroplasts ◽  
Spinach Leaf

Endogenous biosynthetic precursors of (+)-abscisic acid. VI. Carotenoids and ABA are formed by the ‘non-mevalonate’ triose-pyruvate pathway in chloroplasts

1998 ◽  
Vol 25 (5) ◽  
pp. 507 ◽  
Author(s):  
B.V. Milborrow ◽  
H.-S. Lee
Keyword(s):  
Abscisic Acid ◽  
Leaf Tissue ◽  
Isopentenyl Diphosphate ◽  
Free System ◽  
Cell Free System ◽  
Avocado Fruit ◽  
Intact Chloroplasts ◽  
A Cell ◽  
Spinach Leaf ◽  
Leaf Protoplasts

A cell-free system from avocado fruit which routinely incorporated [14C]mevalonate into ABA (1000 dpm per 5 mL of preparation), and into carotenoids, has now been shown to incorporate [14C]pyruvate even more successfully (1620 dpm). Intact chloroplasts from spinach leaf protoplasts incorporated 2990 dpm of [14C]pyruvate (from 2 x 106 dpm) into ABA compared with 990 dpm from [3-R-5-14C]mevalonate (also from 2 x 106 dpm). The intact chloroplasts also produced [14C]ABA (1575 dpm) when supplied with [14C]isopentenyl diphosphate. This result establishes that the whole pathway of biosynthesis of ABA can occur within chloroplasts. Little [14C]acetate or [14C]alanine was incorporated into ABA by avocado fruit mesocarp. Most of the ABA in leaf tissue now appears to be formed by the triose-pyruvate pathway in chloroplasts and incorporation of [14C]mevalonate occurs after activation in the cytoplasm and importation of a later intermediate into the plastids.

Variation in Chloroplast Lipid Content and its Correlation to Photosynthetic Activities

1978 ◽  
Vol 33 (9-10) ◽  
pp. 685-687 ◽  
Author(s):  
K.-P. Heise
Keyword(s):  
Membrane Lipids ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Lipid Synthesis ◽  
Neutral Lipid Fraction ◽  
Intact Chloroplasts ◽  
Spinach Leaf ◽  
Fixation Capacity ◽  
Photosynthetic Activities ◽  
Proportional Decrease

Abstract During illumination infiltrated spinach leaf sections show after a short log a linearly increasing 14C-incorporation into membrane lipids from H14CO3-.Isolated intact chloroplasts show this increase only for the neutral lipid fraction (NL). Glyco-and phospholipid accumulation declines in the light.In chloroplast suspensions the increase in content and also in its 14C-incorporation rate of the NL fraction is accompanied1. by a proportional decrease of CO2-fixation capacity,2. by a decrease of the P/2 e-quotient.From 14C-incorporation kinetics, this increase of neutral lipids in plastid suspensions even after short illumination periods appears to be a consequence of an inhibited glycolipid - and phospho­ lipid synthesis from diglycerides.

scholarly journals Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf Protoplasts

1976 ◽  
Vol 58 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Mikio Nishimura ◽  
Douglas Graham ◽  
Takashi Akazawa
Keyword(s):  
Cell Organelles ◽  
Intact Chloroplasts ◽  
Spinach Leaf ◽  
Leaf Protoplasts

Synthesis Process and Structure of Al/Mg Modified Silica-gel Particle.

2000 ◽  
Vol 116 (1) ◽  
pp. 56-61
Author(s):  
Tatsuo YAMAGUCHI ◽  
Toshiaki NAKAI ◽  
Kunihiko TAKEDA
Keyword(s):  
Silica Gel ◽  
Synthesis Process ◽  
Modified Silica ◽  
Modified Silica Gel

A novel immobilized trypsin on hydrophilic polymer modified silica beads for proteome characterization

2013 ◽  
Vol 31 (5) ◽  
pp. 423
Author(s):  
Chao FAN ◽  
Zifeng SONG ◽  
Weijie QIN ◽  
Yun CAI ◽  
Xiaohong QIAN
Keyword(s):  
Hydrophilic Polymer ◽  
Modified Silica ◽  
Immobilized Trypsin ◽  
Silica Beads

Sodium Chloride Modified Silica Nanoparticles as a Non-Viral Vector with a High Efficiency of DNA Transfer into Cells

2003 ◽  
Vol 3 (3) ◽  
pp. 273-279 ◽  
Author(s):  
Yuxiang Chen ◽  
Zhigang Xue ◽  
Duo Zheng ◽  
Kun Xia ◽  
Yanzhong Zhao ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Silica Nanoparticles ◽  
High Efficiency ◽  
Viral Vector ◽  
Dna Transfer ◽  
Modified Silica ◽  
Modified Silica Nanoparticles

Temperature-modulated cell-separation column using temperature-responsive cationic copolymer hydrogel-modified silica beads

2019 ◽  
Vol 178 ◽  
pp. 253-262 ◽  
Author(s):  
Kenichi Nagase ◽  
Daimu Inanaga ◽  
Daiju Ichikawa ◽  
Aya Mizutani Akimoto ◽  
Yutaka Hattori ◽  
...  
Keyword(s):  
Cell Separation ◽  
Modified Silica ◽  
Copolymer Hydrogel ◽  
Temperature Responsive ◽  
Separation Column ◽  
Silica Beads

Palladium nanoparticles on amino-modified silica-catalyzed C–C bond formation with carbonyl insertion

2021 ◽  
Author(s):  
Elham Etemadi-Davan ◽  
Dariush Khalili ◽  
Ali Reza Banazadeh ◽  
Ghazal Sadri ◽  
Pourya Arshad
Keyword(s):  
Palladium Nanoparticles ◽  
Bond Formation ◽  
Modified Silica
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