scholarly journals Biosynthesis of L-lysine and L-threonine in Brevibacterium. I. Pathway and regulation of lysine biosynthesis in Brevibacterium lactofermentum.

1978 ◽  
Vol 42 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Osamu TOSAKA ◽  
Koichi TAKINAMI
Keyword(s):  
Lysine Biosynthesis ◽  
Brevibacterium Lactofermentum

scholarly journals Regulation of lysine biosynthesis by leucine in Brevibacterium lactofermentum.

1978 ◽  
Vol 42 (8) ◽  
pp. 1501-1506 ◽  
Author(s):  
Osamu TOSAKA ◽  
Hayao HIRAKAWA ◽  
Koichi TAKINAMI ◽  
Yoshio HIROSE
Keyword(s):  
Lysine Biosynthesis ◽  
Brevibacterium Lactofermentum

Regulation of Lysine Biosynthesis by Leucine in Brevibacterium lactofermentum

1978 ◽  
Vol 42 (8) ◽  
pp. 1501-1506
Author(s):  
Osamu Tosaka ◽  
Hayao Hirakawa ◽  
Koichi Takinami ◽  
Yoshio Hirose
Keyword(s):  
Lysine Biosynthesis ◽  
Brevibacterium Lactofermentum

scholarly journals Enzymic Reduction of α-Aminoadipic Acid: Relation to Lysine Biosynthesis

1963 ◽  
Vol 238 (1) ◽  
pp. 275-282
Author(s):  
R.L. Larson ◽  
W.D. Sandine ◽  
Harry P. Broquist
Keyword(s):  
Lysine Biosynthesis

scholarly journals Lysine Biosynthesis in Yeast: A New Metabolite of α-Aminoadipic Acid

1964 ◽  
Vol 239 (2) ◽  
pp. 508-515
Author(s):  
M.H. Kuo ◽  
P.P. Saunders ◽  
Harry P. Broquist
Keyword(s):  
Lysine Biosynthesis

scholarly journals Bayesian Network Analysis of Lysine Biosynthesis Pathway in Rice

Inventions ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 37
Author(s):  
Aditya Lahiri ◽  
Khushboo Rastogi ◽  
Aniruddha Datta ◽  
Endang M. Septiningsih
Keyword(s):  
Amino Acids ◽  
Reporter Gene ◽  
Nacl Stress ◽  
Stress Conditions ◽  
Lysine Biosynthesis ◽  
Model Parameters ◽  
Saline Stress ◽  
Lysine Content ◽  
Biosynthesis Pathway ◽  
Healthy Functioning

Lysine is the first limiting essential amino acid in rice because it is present in the lowest quantity compared to all the other amino acids. Amino acids are the building block of proteins and play an essential role in maintaining the human body’s healthy functioning. Rice is a staple food for more than half of the global population; thus, increasing the lysine content in rice will help improve global health. In this paper, we studied the lysine biosynthesis pathway in rice (Oryza sativa) to identify the regulators of the lysine reporter gene LYSA (LOC_Os02g24354). Genetically intervening at the regulators has the potential to increase the overall lysine content in rice. We modeled the lysine biosynthesis pathway in rice seedlings under normal and saline (NaCl) stress conditions using Bayesian networks. We estimated the model parameters using experimental data and identified the gene DAPF(LOC_Os12g37960) as a positive regulator of the lysine reporter gene LYSA under both normal and saline stress conditions. Based on this analysis, we conclude that the gene DAPF is a potent candidate for genetic intervention. Upregulating DAPF using methods such as CRISPR-Cas9 gene editing strategy has the potential to upregulate the lysine reporter gene LYSA and increase the overall lysine content in rice.

scholarly journals Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 952
Author(s):  
Paula Bellés-Sancho ◽  
Martina Lardi ◽  
Yilei Liu ◽  
Sebastian Hug ◽  
Marta Adriana Pinto-Carbó ◽  
...  
Keyword(s):  
Nitrogenase Activity ◽  
Root Nodules ◽  
Growth Conditions ◽  
Lysine Biosynthesis ◽  
Metabolome Analysis ◽  
Plant Host ◽  
Free Living ◽  
Bacterial Enzyme ◽  
Homocitrate Synthase ◽  
Iron Molybdenum Cofactor

Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided to bacteroids in root nodules by their plant host. In contrast, non-nodulating free-living diazotrophs encode the homocitrate synthase (NifV) and reduce N2 in nitrogen-limiting free-living conditions. Paraburkholderia phymatum STM815 is a beta-rhizobial strain, which can enter symbiosis with a broad range of legumes, including papilionoids and mimosoids. In contrast to most alpha-rhizobia, which lack nifV, P. phymatum harbors a copy of nifV on its symbiotic plasmid. We show here that P. phymatum nifV is essential for nitrogenase activity both in root nodules of papilionoid plants and in free-living growth conditions. Notably, nifV was dispensable in nodules of Mimosa pudica despite the fact that the gene was highly expressed during symbiosis with all tested papilionoid and mimosoid plants. A metabolome analysis of papilionoid and mimosoid root nodules infected with the P. phymatum wild-type strain revealed that among the approximately 400 measured metabolites, homocitrate and other metabolites involved in lysine biosynthesis and degradation have accumulated in all plant nodules compared to uninfected roots, suggesting an important role of these metabolites during symbiosis.

Sign in / Sign up

Export Citation Format

Share Document