scholarly journals Influence of Monochromatic Light on the Action of Enzymes

1940 ◽  
Vol 16 (4) ◽  
pp. 55-68
Author(s):  
Reitaro MURAKAMI
Keyword(s):  
Monochromatic Light

Instrumentation for detecting channelling radiation in the high-voltage electron microscope

1983 ◽  
Vol 41 ◽  
pp. 318-319
Author(s):  
J. H. Butler ◽  
C. J. Humphreys
Keyword(s):  
Monochromatic Light ◽  
Incident Beam ◽  
Laboratory Frame ◽  
Relativistic Electrons ◽  
Voltage Electron ◽  
Dipole Emission ◽  
Sinusoidal Oscillations ◽  
Pass Through ◽  
Incident Beam Energy ◽  
Increasing Function

Electromagnetic radiation is emitted when fast (relativistic) electrons pass through crystal targets which are oriented in a preferential (channelling) direction with respect to the incident beam. In the classical sense, the electrons perform sinusoidal oscillations as they propagate through the crystal (as illustrated in Fig. 1 for the case of planar channelling). When viewed in the electron rest frame, this motion, a result of successive Bragg reflections, gives rise to familiar dipole emission. In the laboratory frame, the radiation is seen to be of a higher energy (because of the Doppler shift) and is also compressed into a narrower cone of emission (due to the relativistic “searchlight” effect). The energy and yield of this monochromatic light is a continuously increasing function of the incident beam energy and, for beam energies of 1 MeV and higher, it occurs in the x-ray and γ-ray regions of the spectrum. Consequently, much interest has been expressed in regard to the use of this phenomenon as the basis for fabricating a coherent, tunable radiation source.

scholarly journals Interfacial chemical bond and internal electric field modulated Z-scheme Sv-ZnIn2S4/MoSe2 photocatalyst for efficient hydrogen evolution

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xuehua Wang ◽  
Xianghu Wang ◽  
Jianfeng Huang ◽  
Shaoxiang Li ◽  
Alan Meng ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Electric Field ◽  
Hydrogen Evolution ◽  
Density Functional ◽  
Monochromatic Light ◽  
Density Functional Theory Calculations ◽  
Internal Electric Field ◽  
Apparent Quantum Yield ◽  
Paramagnetic Resonance ◽  
High Hydrogen

AbstractConstruction of Z-scheme heterostructure is of great significance for realizing efficient photocatalytic water splitting. However, the conscious modulation of Z-scheme charge transfer is still a great challenge. Herein, interfacial Mo-S bond and internal electric field modulated Z-scheme heterostructure composed by sulfur vacancies-rich ZnIn2S4 and MoSe2 was rationally fabricated for efficient photocatalytic hydrogen evolution. Systematic investigations reveal that Mo-S bond and internal electric field induce the Z-scheme charge transfer mechanism as confirmed by the surface photovoltage spectra, DMPO spin-trapping electron paramagnetic resonance spectra and density functional theory calculations. Under the intense synergy among the Mo-S bond, internal electric field and S-vacancies, the optimized photocatalyst exhibits high hydrogen evolution rate of 63.21 mmol∙g−1·h−1 with an apparent quantum yield of 76.48% at 420 nm monochromatic light, which is about 18.8-fold of the pristine ZIS. This work affords a useful inspiration on consciously modulating Z-scheme charge transfer by atomic-level interface control and internal electric field to signally promote the photocatalytic performance.

scholarly journals Mouse embryonic stem cell-derived cardiomyocytes cease to beat following exposure to monochromatic light: association with increased ROS and loss of calcium transients

2019 ◽  
Vol 317 (4) ◽  
pp. C725-C736
Author(s):  
Gurbind Singh ◽  
Divya Sridharan ◽  
Mahmood Khan ◽  
Polani B. Seshagiri
Keyword(s):  
Cell Biology ◽  
Fluorescent Protein ◽  
Es Cells ◽  
Monochromatic Light ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Medical Diagnostics ◽  
Calcium Transients ◽  
Egfp Expression ◽  
Mitochondrial Activity

We earlier established the mouse embryonic stem (ES) cell “GS-2” line expressing enhanced green fluorescent protein (EGFP) and have been routinely using it to understand the molecular regulation of differentiation into cardiomyocytes. During such studies, we made a serendipitous discovery that functional cardiomyocytes derived from ES cells stopped beating when exposed to blue light. We observed a gradual cessation of contractility within a few minutes, regardless of wavelength (nm) ranges tested: blue (~420–495), green (~510–575), and red (~600–700), with green light manifesting the strongest impact. Following shifting of cultures back into the incubator (darkness), cardiac clusters regained beatings within a few hours. The observed light-induced contractility-inhibition effect was intrinsic to cardiomyocytes and not due to interference from other cell types. Also, this was not influenced by any physicochemical parameters or intracellular EGFP expression. Interestingly, the light-induced cardiomyocyte contractility inhibition was accompanied by increased intracellular reactive oxygen species (ROS), which could be abolished in the presence of N-acetylcysteine (ROS quencher). Besides, the increased intracardiomyocyte ROS levels were incidental to the inhibition of calcium transients and suppression of mitochondrial activity, both being essential for sarcomere function. To the best of our knowledge, ours is the first report to demonstrate the monochromatic light-mediated inhibition of contractions of cardiomyocytes with no apparent loss of cell viability and contractility. Our findings have implications in cardiac cell biology context in terms of 1) mechanistic insights into light impact on cardiomyocyte contraction, 2) potential use in laser beam-guided (cardiac) microsurgery, photo-optics-dependent medical diagnostics, 3) transient cessation of hearts during coronary artery bypass grafting, and 4) functional preservation of hearts for transplantation.

BRIGHTNESS SCALES FOR MONOCHROMATIC LIGHT

1960 ◽  
Vol 1 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Gösta Ekman ◽  
Hannes Eisler ◽  
Teodor Künnapas
Keyword(s):  
Monochromatic Light

Decay of photo-induced conductivity in Sb-doped SnO2 thin films, using monochromatic light of about bandgap energy

2013 ◽  
Vol 267 ◽  
pp. 164-168 ◽  
Author(s):  
E.A. Floriano ◽  
L.V.A. Scalvi ◽  
J.R. Sambrano ◽  
A. de Andrade
Keyword(s):  
Thin Films ◽  
Monochromatic Light ◽  
Bandgap Energy ◽  
Sno2 Thin Films

Wavelength-dependent temporal properties of retinal horizontal cells in turtles

1990 ◽  
Vol 4 (05) ◽  
pp. 427-435 ◽  
Author(s):  
C.A. Dvorak ◽  
A.M. Granda
Keyword(s):  
Temporal Integration ◽  
Monochromatic Light ◽  
Maximum Amplitude ◽  
Light Response ◽  
Chelonia Mydas ◽  
Sea Turtle ◽  
Horizontal Cells ◽  
Freshwater Turtle ◽  
L Cells ◽  
Pseudemys Scripta

AbstractElectrical reponses of luminosity horizontal cells (L cells) to monochromatic stimuli were analyzed by intracellular recordings in the retinas of the freshwater turtle (Pseudemys scripta elegans) and of the sea turtle (Chelonia mydas mydas). Light intensity, duration, and wavelength were varied to assess temporal effects. For a given intensity of monochromatic light, response amplitude increased with stimulus duration until maximum amplitude occurred at a specific duration. This suprathreshold metric of temporal integration is calledhere summation time, and it is wavelength-dependent.L cells always had some level of red-sensitive cone input, although in some cells inputs from green- and blue-sensitive cones were also observed. For these latter cells, summation times were shorter for 640-nm than for 540-nm or 450-nm lights. These results were most evident in cells that received dominant inputs from blue- or green-sensitive cones.Responses of some other L cells were almost completely dominated by inputs from red-sensitive cones. Summation times of these cells were not wavelength-dependent. But when these inputs also included green-sensitive cones, shorter summation times were obtained to 640-nm light than to 540-nm light, even though dominant inputs were still from red-sensitive cones. These results, obtained from both retinal and 3,4-dehydroretinal photopigment systems, are consistent with reported observations inPseudemys scripta elegansthat show linear responses of red-sensitive cones to have shorterintegration times and times-to-peakthan green-sensitive cones.Responses from horizontal cells dominated by blue-sensitive cone inputs were the most sensitive of all; they also had the longest summation times. These results support the hypothesis that a gain in sensitivity occurs from the integration of absorbed photons over longer periods of time.These intracellular responses are of particular importance because behavioral critical durations in turtle, as defined by Bloch&'s law, are similarly wavelength-dependent.

Do changes in light direction affect absorption profiles in leaves?

2010 ◽  
Vol 37 (5) ◽  
pp. 403 ◽  
Author(s):  
Craig R. Brodersen ◽  
Thomas C. Vogelmann
Keyword(s):  
Cross Sections ◽  
Incident Light ◽  
Monochromatic Light ◽  
Diffuse Light ◽  
Spongy Mesophyll ◽  
Light Regimes ◽  
Direct Light ◽  
Direct Measurements ◽  
Leaf Level

Leaf anatomy plays a functional role in propagating light through the leaf; palisade mesophyll has been shown to facilitate the channelling of collimated light deeper into the spongy mesophyll. Direct measurements of the propagation of diffuse light into the leaf, however, are absent. Using chlorophyll fluorescence imaging of leaf cross-sections, we measured light absorption profiles in leaves under direct (collimated), diffuse and low-angle monochromatic light. Low-angle and diffuse light was absorbed closer to the irradiated surface than direct light perpendicular to the surface. The shapes of internal absorption profiles indicated that leaves were influenced by the directional quality of the incident light. In addition, absorption profiles revealed that leaves were not simple light absorbing objects and that cellular anatomy influences the direction of light travelling into the mesophyll. These findings also suggest a mechanism for previously measured differences in leaf level photosynthesis under opposing light regimes.

scholarly journals Photocatalytic Activity: Experimental Features to Report in Heterogeneous Photocatalysis

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1990 ◽  
Author(s):  
Md. Hoque ◽  
Marcelo Guzman
Keyword(s):  
Reaction Rate ◽  
Monochromatic Light ◽  
Heterogeneous Photocatalysis ◽  
Research Articles ◽  
Quantum Yields ◽  
Characterization Methods ◽  
Valuable Data ◽  
Photocatalytic Activities ◽  
Scientific Papers

Heterogeneous photocatalysis is a prominent area of research with major applications in solar energy conversion, air pollution mitigation, and removal of contaminants from water. A large number of scientific papers related to the photocatalysis field and its environmental applications are published in different journals specializing in materials and nanomaterials. However, many problems exist in the conception of papers by authors unfamiliar with standard characterization methods of photocatalysts as well as with the procedures needed to determine photocatalytic activities based on the determination of “apparent quantum efficiencies” within a wavelength interval or “apparent quantum yields” in the case of using monochromatic light. In this regard, an astonishing number of recent research articles include claims of highly efficient (photo)catalysts or similar terms about materials with superior or enhanced efficiency for a given reaction without proper experimental support. Consequently, the comparison of the efficiencies of photocatalysts may result as being meaningless, especially when reports are only based on expressions determining (1) a reaction rate per weight of catalyst or its surface area, (2) quantum efficiencies or quantum yields, and (3) turnover frequencies or turnover numbers. Herein, we summarize the standards needed for reporting valuable data in photocatalysis and highlight some common discrepancies found in the literature. This work should inform researchers interested in reporting photocatalysis projects about the correct procedures for collecting experimental data and properly characterizing the materials by providing examples and key supporting literature.

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