Generation of a Zinc Finger Protein ZPR1 Mutant That Constitutively Interacted with Translation Elongation Factor 1α

2009 ◽  
Vol 73 (12) ◽  
pp. 2809-2811 ◽  
Author(s):  
Noriyuki YANAKA ◽  
Yurika KASEDA ◽  
Anna TANAKA ◽  
Yoshihito NOGUSA ◽  
Naoki SUMIYOSHI ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Finger Protein ◽  
Translation Elongation Factor 1Α

scholarly journals Interaction of ZPR1 with Translation Elongation Factor-1α in Proliferating Cells

1998 ◽  
Vol 143 (6) ◽  
pp. 1471-1484 ◽  
Author(s):  
Laxman Gangwani ◽  
Monique Mikrut ◽  
Zoya Galcheva-Gargova ◽  
Roger J. Davis
Keyword(s):  
Mammalian Cells ◽  
Cellular Proliferation ◽  
Zinc Finger Protein ◽  
Elongation Factor ◽  
Normal Growth ◽  
Terminal Region ◽  
Translation Elongation Factor ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Translation Elongation Factor 1Α

The zinc finger protein ZPR1 is present in the cytoplasm of quiescent mammalian cells and translocates to the nucleus upon treatment with mitogens, including epidermal growth factor (EGF). Homologues of ZPR1 were identified in yeast and mammals. These ZPR1 proteins bind to eukaryotic translation elongation factor-1α (eEF-1α). Studies of mammalian cells demonstrated that EGF treatment induces the interaction of ZPR1 with eEF-1α and the redistribution of both proteins to the nucleus. In the yeast Saccharomyces cerevisiae, genetic analysis demonstrated that ZPR1 is an essential gene. Deletion analysis demonstrated that the NH2-terminal region of ZPR1 is required for normal growth and that the COOH-terminal region was essential for viability in S. cerevisiae. The yeast ZPR1 protein redistributes from the cytoplasm to the nucleus in response to nutrient stimulation. Disruption of the binding of ZPR1 to eEF-1α by mutational analysis resulted in an accumulation of cells in the G2/M phase of cell cycle and defective growth. Reconstitution of the ZPR1 interaction with eEF-1α restored normal growth. We conclude that ZPR1 is essential for cell viability and that its interaction with eEF-1α contributes to normal cellular proliferation.

scholarly journals Muerte descendente de Prunus sp. asociada a Armillaria spp. en huertas comerciales de Michoacán, México

2017 ◽  
Vol 35 (3) ◽  
Author(s):  
Patricia Rivas-Valencia ◽  
Alejandra Almaraz-Sánchez ◽  
Adriana Cano-Salgado ◽  
Lervin Hernández-Ramos ◽  
Iobana Alanís-Martínez ◽  
...  
Keyword(s):  
Prunus Persica ◽  
Elongation Factor ◽  
Prunus Domestica ◽  
Translation Elongation Factor ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Translation Elongation Factor 1Α

<p>El área productora de durazno (<em>Prunus persica</em>) y ciruelo (<em>Prunus domestica</em>) en Michoacán, se localiza en el municipio de Zinapécuaro. Recientemente se han presentado síntomas de un deterioro progresivo de los árboles que les ocasiona la muerte, poniendo en riesgo la producción de fruta. El objetivo de este estudio fue identificar y caracterizar molecularmente el patógeno responsable. En las localidades de Ucareo, Jeráhuaro, Huajúmbaro, San Joaquín y La Galera con antecedentes del problema, se colectaron muestras de raíz, tronco y suelo. Se identificaron signos característicos del hongo <em>Armillaria</em> spp., confirmado por secuenciación molecular del gen Tef-1α (translation elongation factor 1α). El análisis filogenético agrupó las secuencias como dos especies nuevas, filogenéticamente emparentada en el mismo clado que <em>A. mellea</em>, que es una especie patógena agresiva. En México no se cuenta actualmente con portainjertos tolerantes, y por tanto, se recomienda continuar con estudios de búsqueda de resistencia genética y manejo de la enfermedad en las huertas.</p>

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