scholarly journals Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms

2010 ◽  
Vol 74 (2) ◽  
pp. 343-349 ◽  
Author(s):  
Masaki SHINTANI ◽  
Hisakazu YAMANE ◽  
Hideaki NOJIRI
Keyword(s):  
Degradative Plasmid

Role of the nucleoid-associated proteins governing the interaction between host chromosome and carbazole degradative plasmid pCAR1

2010 ◽  
Vol 150 ◽  
pp. 259-260
Author(s):  
Chiho Suzuki ◽  
Choong-Soo Yun ◽  
Tohru Terada ◽  
Kazuya Watanabe ◽  
Hisakazu Yamane ◽  
...  
Keyword(s):  
Host Chromosome ◽  
Associated Proteins ◽  
Degradative Plasmid

scholarly journals Characterisation of a degradative plasmid inPseudomonas putidathat controls the expression of 2,4-xylenol degradative genes

1989 ◽  
Vol 61 (1-2) ◽  
pp. 153-158 ◽  
Author(s):  
H.F. Dean ◽  
S. Cheevadhanarak ◽  
R.A. Skurray ◽  
R.C. Bayly
Keyword(s):  
Degradative Plasmid

A novel dichloromethane-degrading Lysinibacillus sphaericus strain wh22 and its degradative plasmid

2009 ◽  
Vol 82 (4) ◽  
pp. 731-740 ◽  
Author(s):  
Shijin J. Wu ◽  
Zhihang H. Hu ◽  
Lili L. Zhang ◽  
Xiang Yu ◽  
Jianmeng M. Chen
Keyword(s):  
Lysinibacillus Sphaericus ◽  
Degradative Plasmid

The meta cleavage operon of TOL degradative plasmid pWWO comprises 13 genes

1990 ◽  
Vol 221 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Shigeaki Harayama ◽  
Monique Rekik
Keyword(s):  
Degradative Plasmid

scholarly journals Transcriptional Regulation of the ant Operon, Encoding Two-Component Anthranilate 1,2-Dioxygenase, on the Carbazole-Degradative Plasmid pCAR1 of Pseudomonas resinovorans Strain CA10

2004 ◽  
Vol 186 (20) ◽  
pp. 6815-6823 ◽  
Author(s):  
Masaaki Urata ◽  
Masatoshi Miyakoshi ◽  
Satoshi Kai ◽  
Kana Maeda ◽  
Hiroshi Habe ◽  
...  
Keyword(s):  
Transcriptional Unit ◽  
Northern Hybridization ◽  
Luciferase Expression ◽  
Transcription Start Point ◽  
Rt Pcr ◽  
Transcription Start ◽  
Pseudomonas Resinovorans ◽  
Two Component ◽  
Degradative Plasmid ◽  
Strain Ca10

ABSTRACT The carbazole-degradative plasmid pCAR1 of Pseudomonas resinovorans strain CA10 has two gene clusters, carAaAaBaBbCAcAdDFE and antABC, which are involved in the conversions of carbazole to anthranilate and anthranilate to catechol, respectively. We proved that the antABC gene cluster, encoding two-component anthranilate 1,2-dioxygenase, constitutes a single transcriptional unit through Northern hybridization and reverse transcription-PCR (RT-PCR) analyses. The transcription start point of antA was mapped at 53 bp upstream point of its translation start point, and the −10 and −35 boxes were homologous to conserved σ70 recognition sequence. Hence the promoter of the ant operon was designated P ant . 5′ Deletion analyses using luciferase as a reporter showed that the region up to at least 70 bp from the transcription start point of antA was necessary for the activation of P ant . Luciferase expression from P ant was induced by anthranilate itself, but not by catechol. Two probable AraC/XylS-type regulatory genes found on pCAR1, open reading frame 22 (ORF22) and ORF23, are tandemly located 3.2 kb upstream of the antA gene. We revealed that the product of ORF23, designated AntR, is indispensable for the stimulation of P ant in Pseudomonas putida cells. Northern hybridization and RT-PCR analyses revealed that another copy of P ant , which is thought to be translocated about 2.1 kb upstream of the carAa gene as a consequence of the transposition of ISPre1, actually drives transcription of the carAa gene in the presence of anthranilate, indicating that both ant and car operons are simultaneously regulated by AntR.

scholarly journals Evolution of the IncP-7 carbazole-degradative plasmid pCAR1 improves survival of its host Pseudomonas fluorescens Pf0-1 in artificial water microcosms

Microbiology ◽  
2011 ◽  
Vol 157 (8) ◽  
pp. 2276-2286 ◽  
Author(s):  
Masaki Shintani ◽  
Tadafumi Horisaki ◽  
Hisakazu Yamane ◽  
Moriya Ohkuma ◽  
Hideaki Nojiri
Keyword(s):  
Pseudomonas Fluorescens ◽  
Parent Strain ◽  
Pcr Analysis ◽  
Post Inoculation ◽  
Rt Pcr ◽  
Mutant Strains ◽  
Repetitive Extragenic Palindromic Pcr ◽  
Degradative Plasmid ◽  
Transcriptional Induction ◽  
Artificial Water

In our previous study, Pseudomonas fluorescens Pf0-1L, harbouring the IncP-7 carbazole-degradative plasmid pCAR1 : : rfp, was shown to be undetectable within 5 days post-inoculation in carbazole-contaminated artificial freshwater microcosms containing several plasmid-free bacteria in addition to Pf0-1L(pCAR1 : : rfp). Fourteen days after the inoculation, carbazole degraders become detectable. Here, we revealed that these isolates were not pCAR1 transconjugants, but Pf0-1L(pCAR1 : : rfp) mutants, based on RFLP and BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) analysis. Notably, the mutants displayed more rapid initiation of carbazole degradation than the parent strain Pf0-1L(pCAR1 : : rfp). The mutants were unable to degrade anthranilate due to a 163 bp deletion in the antA gene, which was overcome by their transformation with a wild-type antABC-expressing plasmid. Quantitative RT-PCR analysis indicated that the transcriptional induction of carbazole-, anthranilate- and catechol-degradative genes was comparable in both parent and mutant strains. The deletion mutants became dominant in the artificial water microcosm. The mutation caused anthranilate to accumulate instead of catechol, a toxic compound for the parent strain, and may be beneficial to host survival in artificial microcosms.

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