Repeated Batch Production of Theanine by Coupled Fermentation with Energy Transfer Using Membrane-Enclosed γ-Glutamylmethylamide Synthetase and Dried Yeast Cells

2009 ◽  
Vol 73 (12) ◽  
pp. 2800-2802 ◽  
Author(s):  
Sachiko YAMAMOTO ◽  
Yosuke MORIHARA ◽  
Mamoru WAKAYAMA ◽  
Takashi TACHIKI
Keyword(s):  
Energy Transfer ◽  
Yeast Cells ◽  
Batch Production ◽  
Repeated Batch

scholarly journals Effect of Washing Yeast Cells on Alcohol Productivity in Repeated Batch Fermentation in Beet Molasses

1988 ◽  
Vol 83 (7) ◽  
pp. 501-503
Author(s):  
Kazuhito MORIYA ◽  
Haruyuki IEFUJI ◽  
Hitoshi SHIMOI ◽  
Shun-ichi SATO ◽  
Makoto TADENUMA
Keyword(s):  
Batch Fermentation ◽  
Yeast Cells ◽  
Beet Molasses ◽  
Repeated Batch Fermentation ◽  
Repeated Batch

scholarly journals Designing and Development of FRET-Based Nanosensor for Real Time Analysis of N-Acetyl-5-Neuraminic Acid in Living Cells

2021 ◽  
Vol 8 ◽  
Author(s):  
Ruphi Naz ◽  
Mohammad K. Okla ◽  
Urooj Fatima ◽  
Mohd. Mohsin ◽  
Walid H. Soufan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Metabolic Engineering ◽  
Sialic Acid ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Neuraminic Acid ◽  
Yeast Cells ◽  
Metabolite Level ◽  
Health Maintenance

N-acetyl-5-neuraminic acid (NeuAc) plays crucial role in improving the growth, brain development, brain health maintenance, and immunity enhancement of infants. Commercially, it is used in the production of antiviral drugs, infant milk formulas, cosmetics, dietary supplements, and pharmaceutical products. Because of the rapidly increasing demand, metabolic engineering approach has attracted increasing attention for NeuAc biosynthesis. However, knowledge of metabolite flux in biosynthetic pathways is one of the major challenges in the practice of metabolic engineering. So, an understanding of the flux of NeuAc is needed to determine its cellular level at real time. The analysis of the flux can only be performed using a tool that has the capacity to measure metabolite level in cells without affecting other metabolic processes. A Fluorescence Resonance Energy Transfer (FRET)-based genetically-encoded nanosensor has been generated in this study to monitor the level of NeuAc in prokaryotic and eukaryotic cells. Sialic acid periplasmic binding protein (SiaP) from Haemophilus influenzae was exploited as a sensory element for the generation of nanosensor. The enhanced cyan fluorescent protein (ECFP) and Venus were used as Fluroscence Resonance Energy Transfer (FRET) pair. The nanosensor, which was termed fluorescent indicator protein for sialic acid (FLIP-SA), was successfully transformed into, and expressed in Escherichia coli BL21 (DE3) cells. The expressed protein of the nanosensor was isolated and purified. The purified nanosensor protein was characterized to assess the affinity, specificity, and stability in the pH range. The developed nanosensor exhibited FRET change after addition to NeuAc. The developed nanosensor was highly specific, exhibited pH stability, and detected NeuAc levels in the nanomolar to milimolar range. FLIP-SA was successfully introduced in bacterial and yeast cells and reported the real-time intracellular levels of NeuAc non-invasively. The FLIP-SA is an excellent tool for the metabolic flux analysis of the NeuAc biosynthetic pathway and, thus, may help unravel the regulatory mechanism of the metabolic pathway of NeuAc. Furthermore, FLIP-SA can be used for the high-throughput screening of E. coli mutant libraries for varied NeuAc production levels.

Repeated batch production ofL-phenylalanine from phenylpyruvate and NH4Cl by immobilized cells ofNocardia opaca under hydrogen high pressure

1988 ◽  
Vol 31 (8) ◽  
pp. 834-840 ◽  
Author(s):  
Tadashi Matsunaga ◽  
Michio Higashijima ◽  
Anny Sulaswatty ◽  
Shigeo Nishimura ◽  
Takanori Kitamura ◽  
...  
Keyword(s):  
High Pressure ◽  
Immobilized Cells ◽  
Batch Production ◽  
Repeated Batch

Ethanol production by repeated batch culture using yeast cells immobilized within porous cellulose carriers

2000 ◽  
Vol 90 (5) ◽  
pp. 526-529 ◽  
Author(s):  
Akihiko Sakurai ◽  
Yoshifumi Nishida ◽  
Hidenao Saito ◽  
Mikio Sakakibara
Keyword(s):  
Ethanol Production ◽  
Batch Culture ◽  
Yeast Cells ◽  
Repeated Batch Culture ◽  
Repeated Batch

Repeated-batch production of hydrogen using Rhodobacter sphaeroides S10

2012 ◽  
Vol 37 (21) ◽  
pp. 15855-15866 ◽  
Author(s):  
Walailak Pattanamanee ◽  
Wanna Choorit ◽  
Duangporn Kantachote ◽  
Yusuf Chisti
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Batch Production ◽  
Production Of Hydrogen ◽  
Repeated Batch

scholarly journals REPEATED-BATCH PRODUCTION OF LACCASE BY CERIPORIOPSIS SUBVERMISPORA

2013 ◽  
Vol 12 (2) ◽  
pp. 120-128 ◽  
Author(s):  
Daniela Chmelová ◽  
Miroslav Ondrejovič
Keyword(s):  
Carbon Source ◽  
Critical Concentration ◽  
Casein Hydrolysate ◽  
Batch Cultivation ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Batch Production ◽  
Ceriporiopsis Subvermispora ◽  
Repeated Batch

Abstract The aim of this study was to set parameters of repeated-batch cultivation of Ceriporiopsis subvermispora for laccase production and evaluate the efficiency of this type of cultivation for production of selected enzyme. The suitable conditions for repeated-batch cultivation were designed on the base of study of batch cultivation of white-rot fungus C. subvermispora. C. subvermispora was cultivated in media with different concentration of casein hydrolysate as nitrogen source and glucose as carbon source. A suitable concentration of casein hydrolysate to stimulate the laccase production was 1.5 and 2.5 g/L. Laccase production was started at certain critical concentration of glucose (5 g/L). In order to improve laccase production by repeated-batch cultivation of C. subvermispora, glucose was tested in concentration 10 g/L and casein hydrolysate in concentration 1.5 g/L. During a repeated-batch cultivation was measured increase laccase activities from 177.8 to 266 U/L. It was also observed, the cultivation time needed to reach maximum laccase production was shortened to 10 days.

Sign in / Sign up

Export Citation Format

Share Document