Inhibitory Effect of Quercetin Isolated from Rose Hip (Rosa caninaL.) against Melanogenesis by Mouse Melanoma Cells

2009 ◽  
Vol 73 (9) ◽  
pp. 1989-1993 ◽  
Author(s):  
Takashi FUJII ◽  
Morio SAITO
Keyword(s):  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Mouse Melanoma ◽  
Rose Hip

scholarly journals Capsaicin reverses the inhibitory effect of licochalcone A/β-Arbutin on tyrosinase expression in b16 mouse melanoma cells

2018 ◽  
Vol 14 (53) ◽  
pp. 110 ◽  
Author(s):  
Qiang Liu ◽  
Jun-Hui Hong ◽  
Huo-Ji Chen ◽  
Shi-Jian Xiang ◽  
Si-Wei Cao ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Licochalcone A ◽  
Mouse Melanoma ◽  
B16 Mouse Melanoma ◽  
Tyrosinase Expression

scholarly journals Delta-Tocotrienol Causes Decrease of Melanin Content in Mouse Melanoma Cells

2009 ◽  
Vol 55 (2) ◽  
pp. 314-318 ◽  
Author(s):  
Akihiro Michihara ◽  
Sachiyo Morita ◽  
Yae Hirokawa ◽  
Saya Ago ◽  
Kenji Akasaki ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Melanin Content ◽  
Mouse Melanoma

scholarly journals Novel Chemically Modified Curcumin (CMC) Derivatives Inhibit Tyrosinase Activity and Melanin Synthesis in B16F10 Mouse Melanoma Cells

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 674
Author(s):  
Shilpi Goenka ◽  
Francis Johnson ◽  
Sanford R. Simon
Keyword(s):  
Enzyme Activity ◽  
Parent Compound ◽  
Radical Scavenging ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Partial Recovery ◽  
Tyrosinase Inhibitor ◽  
Pyrocatechol Violet ◽  
Mouse Melanoma ◽  
Chemically Modified

Skin hyperpigmentation disorders arise due to excessive production of the macromolecular pigment melanin catalyzed by the enzyme tyrosinase. Recently, the therapeutic use of curcumin for inhibiting tyrosinase activity and production of melanin have been recognized, but poor stability and solubility have limited its use, which has inspired synthesis of curcumin analogs. Here, we investigated four novel chemically modified curcumin (CMC) derivatives (CMC2.14, CMC2.5, CMC2.23 and CMC2.24) and compared them to the parent compound curcumin (PC) for inhibition of in vitro tyrosinase activity using two substrates for monophenolase and diphenolase activities of the enzyme and for diminution of cellular melanogenesis. Enzyme kinetics were analyzed using Lineweaver-Burk and Dixon plots and nonlinear curve-fitting to determine the mechanism for tyrosinase inhibition. Copper chelating activity, using pyrocatechol violet dye indicator assay, and antioxidant activity, using a DPPH radical scavenging assay, were also conducted. Next, the capacity of these derivatives to inhibit tyrosinase-catalyzed melanogenesis was studied in B16F10 mouse melanoma cells and the mechanisms of inhibition were elucidated. Inhibition mechanisms were studied by measuring intracellular tyrosinase activity, cell-free and intracellular α-glucosidase enzyme activity, and effects on MITF protein level and cAMP maturation factor. Our results showed that CMC2.24 showed the greatest efficacy as a tyrosinase inhibitor of all the CMCs and was better than PC as well as a popular tyrosinase inhibitor-kojic acid. Both CMC2.24 and CMC2.23 inhibited tyrosinase enzyme activity by a mixed mode of inhibition with a predominant competitive mode. In addition, CMC2.24 as well as CMC2.23 showed a comparable robust efficacy in inhibiting melanogenesis in cultured melanocytes. Furthermore, after removal of CMC2.24 or CMC2.23 from the medium, we could demonstrate a partial recovery of the suppressed intracellular tyrosinase activity in the melanocytes. Our results provide a proof-of-principle for the novel use of the CMCs that shows them to be far superior to the parent compound, curcumin, for skin depigmentation.

scholarly journals Treatment with Uncaria tomentosa Promotes Apoptosis in B16-BL6 Mouse Melanoma Cells and Inhibits the Growth of B16-BL6 Tumours

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1066
Author(s):  
Ali Zari ◽  
Hajer Alfarteesh ◽  
Carly Buckner ◽  
Robert Lafrenie
Keyword(s):  
Tumour Size ◽  
Melanoma Cells ◽  
Tunel Staining ◽  
Aqueous Extracts ◽  
Ki 67 ◽  
Uncaria Tomentosa ◽  
Mouse Melanoma ◽  
Anti Cancer

Uncaria tomentosa is a medicinal plant native to Peru that has been traditionally used in the treatment of various inflammatory disorders. In this study, the effectiveness of U. tomentosa as an anti-cancer agent was assessed using the growth and survival of B16-BL6 mouse melanoma cells. B16-BL6 cell cultures treated with both ethanol and phosphate-buffered saline (PBS) extracts of U. tomentosa displayed up to 80% lower levels of growth and increased apoptosis compared to vehicle controls. Treatment with ethanolic extracts of Uncaria tomentosa were much more effective than treatment with aqueous extracts. U. tomentosa was also shown to inhibit B16-BL6 cell growth in C57/bl mice in vivo. Mice injected with both the ethanolic and aqueous extracts of U. tomentosa showed a 59 ± 13% decrease in B16-BL6 tumour weight and a 40 ± 9% decrease in tumour size. Histochemical analysis of the B16-BL6 tumours showed a strong reduction in the Ki-67 cell proliferation marker in U. tomentosa-treated mice and a small, but insignificant increase in terminal transferase dUTP nick labelling (TUNEL) staining. Furthermore, U. tomentosa extracts reduced angiogenic markers and reduced the infiltration of T cells into the tumours. Collectively, the results in this study concluded that U. tomentosa has potent anti-cancer activity that significantly inhibited cancer cells in vitro and in vivo.

Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin

Life Sciences ◽  
1992 ◽  
Vol 51 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Lisha Zhang ◽  
Takemi Yoshida ◽  
Yukio Kuroiwa
Keyword(s):  
Melanoma Cells ◽  
Melanin Synthesis ◽  
Mouse Melanoma ◽  
Stimulation Of
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