Effects of Theaflavins on Melanin Biosynthesis in Mouse B16 Melanoma Cells

2009 ◽  
Vol 73 (6) ◽  
pp. 1429-1431 ◽  
Author(s):  
Yuriko YAMAOKA ◽  
Kenji OHGUCHI ◽  
Tomohiro ITOH ◽  
Yoshinori NOZAWA ◽  
Yukihiro AKAO
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells

scholarly journals Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2697
Author(s):  
Thouria Bourhim ◽  
Myra O. Villareal ◽  
Chemseddoha Gadhi ◽  
Hiroko Isoda
Keyword(s):  
Signaling Pathways ◽  
Press Cake ◽  
Global Gene Expression ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Dependent Manner ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Pigmentary Disorders ◽  
Argan Oil

The beneficial effect on health of argan oil is recognized worldwide. We have previously reported that the cake that remains after argan oil extraction (argan press-cake or APC) inhibits melanogenesis in B16 melanoma cells in a time-dependent manner without cytotoxicity. In this study, the global gene expression profile of B16 melanoma cells treated with APC extract was determined in order to gain an understanding of the possible mechanisms of action of APC. The results suggest that APC extract inhibits melanin biosynthesis by down-regulating microphthalmia-associated transcription factor (Mitf) and its downstream signaling pathway through JNK signaling activation, and the inhibition of Wnt/β-catenin and cAMP/PKA signaling pathways. APC extract also prevented the transport of melanosomes by down-regulating Rab27a expression. These results suggest that APC may be an important natural skin whitening product and pharmacological agent used for clinical treatment of pigmentary disorders.

scholarly journals The Inhibitory Effects of Representative Chalcones Contained in Angelica keiskei on Melanin Biosynthesis in B16 Melanoma Cells

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Enos Tangke Arung ◽  
Shoko Furuta ◽  
Kazuhiro Sugamoto ◽  
Kuniyoshi Shimizu ◽  
Hiroya Ishikawa ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Inhibitory Effects ◽  
Melanin Biosynthesis ◽  
Melanin Formation ◽  
B16 Melanoma Cells ◽  
Angelica Keiskei ◽  
Low Cytotoxicity ◽  
Antioxidant Effects

In our effort to find new whitening agents, we evaluated the effects of representative chalcones [4-hydroxyderricin (1), xanthoangelol (2), xanthoangelol H (3), deoxyxanthoangelol H (4), and deoxydihydroxanthoangelol H (5)] contained in the stem of Angelica keiskei on tyrosinase and melanin formation in B16 melanoma cells. In addition, the antioxidant effects of these chalcones in ORAC and DPPH assays were also determined. Interestingly, all chalcones (1–5) inhibit melanin formation in B16 melanoma cells, with low cytotoxicity.

The effect of chlorophorin and its derivative on melanin biosynthesis

Holzforschung ◽  
2005 ◽  
Vol 59 (5) ◽  
pp. 514-518 ◽  
Author(s):  
Enos Tangke Arung ◽  
Keisuke Yoshikawa ◽  
Kuniyoshi Shimizu ◽  
Ryuichiro Kondo
Keyword(s):  
Inhibitory Activity ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibitor ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Bioassay Guided Fractionation ◽  
Unstable Compound ◽  
Potent Inhibitory Activity

Abstract By means of bioassay-guided fractionation using mushroom tyrosinase, a geranylated stilbene, chlorophorin, was characterized as the principal tyrosinase inhibitor in the heartwood of Chlorophora excelsa (Moraceae). It inhibited the oxidation of L-tyrosine and DL-3,4-dihydroxyphenylalanine (DL-DOPA) due to mushroom tyrosinase and melanin biosynthesis on B16 melanoma cells. Chlorophorin, which is a slight yellowish compound, has previously been reported as an unstable compound in light. On the basis of this finding, a chlorophorin derivative [4-(3″,7″-dimethyloctyl)-2′,3,4′,5-tetrahydroxydihydrostilbene; hexahydrochlorophorin] which is colorless, obtained by the hydrogenation of chlorophorin with Pd/C, was also tested to develop a superior material for practical use. Hexahydrochlorophorin showed more potent inhibitory activity on tyrosinase and melanin biosynthesis, and lower cytotoxicity towards B16 melanoma cells than chlorophorin.

Melanin Biosynthesis Inhibitory and Antioxidant Activities of Quercetin-3’-O-β-D-glucoside Isolated from Allium cepa

2011 ◽  
Vol 66 (5-6) ◽  
pp. 209-214 ◽  
Author(s):  
Enos Tangke Arung ◽  
Shoko Furuta ◽  
Hiroya Ishikawa ◽  
Hiroyuki Tanaka ◽  
Kuniyoshi Shimizu
Keyword(s):  
Allium Cepa ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Melanoma Cells ◽  
Oxygen Radical ◽  
B16 Melanoma ◽  
Mushroom Tyrosinase ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Bioassay Guided Fractionation

In the course of searching for new whitening agents, we have found that the methanol extract of dried skin of Allium cepa shows potent melanin biosynthesis inhibitory activity in B16 melanoma cells. Bioassay-guided fractionation led to the isolation of quercetin-3’-O-β- D-glucoside (1) from the methanol extract of dried skin of A. cepa, which inhibited melanin formation in B16 melanoma cells with an IC50 value of 38.8 μM and mushroom tyrosinase with an IC50 value of 6.5 μM using L-tyrosine and 48.5 μM using L-dihydroxyphenylalanine as substrates, respectively. In addition, the antioxidant activity of 1 was evaluated in the oxygen radical absorbance capacity assay; it showed 3.04 μmol Trolox equivalents/mmol. 1 was shown to be a promising ingredient that could be useful for treating hyperpigmentation and for protecting against oxidative stress.

scholarly journals A novel bioactive chalcone of Morus australis inhibits tyrosinase activity and melanin biosynthesis in B16 melanoma cells

2012 ◽  
Vol 61 (10) ◽  
pp. 585-592 ◽  
Author(s):  
Makoto Takahashi ◽  
Kensaku Takara ◽  
Tomonao Toyozato ◽  
Koji Wada
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells

scholarly journals Acanthoic Acid Inhibits Melanogenesis through Tyrosinase Down-regulation and Melanogenic Gene Expression in B16 Melanoma Cells

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801
Author(s):  
Weon-Jong Yoon ◽  
Young-Min Ham ◽  
Hun Seok Yoon ◽  
Wook-Jae Lee ◽  
Nam Ho Lee ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Down Regulation ◽  
Melanin Biosynthesis ◽  
Acanthopanax Koreanum ◽  
B16 Melanoma Cells ◽  
Whitening Agent ◽  
Rate Limiting ◽  
Acanthoic Acid

The aim of this study was to investigate the in vitro inhibitory effects of acanthoic acid (ACAN), isolated from Acanthopanax koreanum, on melanogenesis and its related enzymes such as tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 in B16 melanoma cells. We found that ACAN significantly attenuates melanin synthesis and reduces the activity of intracellular tyrosinase, the rate-limiting melanogenic enzyme. Western blot analysis showed that ACAN also decreases tyrosinase, TRP-1, and TRP-2 protein expression. In addition, ACAN significantly decreased the expression of microphthalmia-associated transcription factor (MITF), a key regulator of melanogenesis. These results indicate that ACAN effectively inhibits melanin biosynthesis through down-regulation of MITF and thus could be useful as a new skin-whitening agent.

scholarly journals Effect of Phlorotannins Isolated From Eisenia bicyclis on Melanogenesis in Mouse B16 Melanoma Cells

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110192
Author(s):  
Yuki Ohno ◽  
Shiori Kondo ◽  
Kiho Tajima ◽  
Toshiyuki Shibata ◽  
Tomohiro Itoh
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Physiological Effects ◽  
Related Protein ◽  
Eisenia Bicyclis ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16 Melanoma Cells ◽  
Anti Cancer ◽  
Tyrosinase Related Protein

Phlorotannins isolated from brown algae, such as Eisena bicyclis, have positive physiological effects, including anti-cancer, anti-inflammatory, and anti-Alzheimer’s disease. Although phlorotannins have been shown to inhibit tyrosinase, an enzyme essential for melanogenesis, their effect on melanogenesis remains unexplored. Thus, we isolated phlorotannins from E. bicyclis and examined their effects on α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in murine B16 melanoma cells. Both fucofuroeckol-A (FF-A) and phlorofucofuroeckol-A (PFF-A) suppressed α-MSH-induced melanogenesis. Neither inhibited human tyrosinase (TYR) activity, but both inhibited tyrosinase-related protein-2 activity. FF-A downregulated the expression of microphthalmia-associated transcription factor and TYR, which subsequently suppressed melanin production. These results suggest that phlorotannins could be beneficial as melanin control drugs for hyperpigmentation disorders.

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