scholarly journals Growth-Promoting Effect of Alginate Oligosaccharides on a Unicellular Marine Microalga,Nannochloropsis oculata

2009 ◽  
Vol 73 (2) ◽  
pp. 450-453 ◽  
Author(s):  
Takeshi YOKOSE ◽  
Toru NISHIKAWA ◽  
Yoshiko YAMAMOTO ◽  
Yasuhiro YAMASAKI ◽  
Kenichi YAMAGUCHI ◽  
...  
Keyword(s):  
Nannochloropsis Oculata ◽  
Promoting Effect ◽  
Marine Microalga ◽  
Alginate Oligosaccharides ◽  
Growth Promoting

Xyloglucan- and cello-oligosaccharides: Antagonists of the growth-promoting effect of H+

1990 ◽  
Vol 80 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Ester P. Lorences ◽  
Gordon J. McDougall ◽  
Stephen C. Fry
Keyword(s):  
Promoting Effect ◽  
Growth Promoting

Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 239-247 ◽  
Author(s):  
T. Metoki ◽  
H. Iwata ◽  
M. Itoh ◽  
M. Kasai ◽  
A. Takajyo ◽  
...  
Keyword(s):  
Calf Serum ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Promoting Effect ◽  
Oocyte Growth ◽  
Preantral Follicles ◽  
Molecular Weights ◽  
Heat Treated ◽  
Growth Promoting ◽  
Follicular Fluids

SummaryWe examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

The growth promoting effect of mammalian thioredoxin and the redox regulation of intestinal epithelial regeneration

2000 ◽  
Vol 118 (4) ◽  
pp. A602
Author(s):  
Shigeo Takaishi ◽  
Mitsutaka Sawada ◽  
Yukari Morita ◽  
Hiroaki Fukuzawa ◽  
Hiroshi Seno ◽  
...  
Keyword(s):  
Redox Regulation ◽  
Intestinal Epithelial ◽  
Promoting Effect ◽  
Epithelial Regeneration ◽  
Growth Promoting

scholarly journals Growth-promoting effect of carbon material upon bacterial cells propagating through a distance.

1997 ◽  
Vol 43 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Michio Matsuhashi ◽  
Katsura Endoh ◽  
Alla N. Pankrushina ◽  
Hiroshi Watanabe ◽  
Mayumi Komiyama ◽  
...  
Keyword(s):  
Carbon Material ◽  
Bacterial Cells ◽  
Promoting Effect ◽  
Growth Promoting

scholarly journals Growth Promoting Effect of Cyclitols on Spruce Tissue Cultures

1962 ◽  
Vol 37 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Carol Steinhart ◽  
Laurens Anderson ◽  
Folke Skoog
Keyword(s):  
Tissue Cultures ◽  
Promoting Effect ◽  
Growth Promoting

scholarly journals PAPA-1 Is a Nuclear Binding Partner of IGFBP-2 and Modulates Its Growth-Promoting Actions

2009 ◽  
Vol 23 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Kenichi Miyako ◽  
Laura J. Cobb ◽  
Malik Francis ◽  
Alden Huang ◽  
Bonnie Peng ◽  
...  
Keyword(s):  
Glutathione S Transferase ◽  
Mouse Embryonic Fibroblasts ◽  
Promoting Effect ◽  
Embryonic Fibroblasts ◽  
Igf Binding Proteins ◽  
Cellular Effects ◽  
Igf Binding ◽  
Growth Promoting ◽  
Interfering Rna

Abstract IGF-binding proteins (IGFBPs) have multiple cellular effects, which occur by both IGF-dependent and -independent mechanisms. IGFBP-2 is involved in the regulation of both normal and carcinogenic cell growth. To further understand the actions of IGFBP-2, we carried out a yeast two-hybrid screen to search for intracellular partner proteins using a human prostate cDNA library. We isolated Pim-1-associated protein-1 (PAP-1)-associated protein-1 (PAPA-1) as an IGFBP-2-binding protein, whose expression and subcellular localization is regulated by both IGFBP-2 and androgens. Coimmunoprecipitation and glutathione S-transferase pull-down assay confirmed the interaction in vitro, and confocal microscopy showed the colocalization of IGFBP-2 and PAPA-1 in the nucleus. Suppression of PAPA-1 by small interfering RNA treatment enhanced the growth-promoting effect of IGFBP-2. Conversely, IGFBP-2-promoted bromodeoxyuridine incorporation into LNCaP cells was abrogated by the simultaneous overexpression of myc-hPAPA-1. Mouse embryonic fibroblasts from IGFBP-2 knockout mouse showed diminished growth activity compared with wild type, and expression of FLAG-mPAPA-1 decreased cell proliferation in IGFBP-2 knockout, but not control mouse embryonic fibroblasts. These studies suggest that the growth-promoting role of IGFBP-2 in prostate cancer is inhibited by its intracellular interaction with PAPA-1.

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