Ginsenoside Compound K Production from Ginseng Root Extract by a Thermostable β-Glycosidase fromSulfolobus solfataricus

2009 ◽  
Vol 73 (2) ◽  
pp. 316-321 ◽  
Author(s):  
Kyeong-Hwan NOH ◽  
Ju-Wan SON ◽  
Hye-Jung KIM ◽  
Deok-Kun OH
Keyword(s):  
Ginseng Root ◽  
Root Extract ◽  
Compound K ◽  
Ginsenoside Compound K

scholarly journals Optimal bioconversion for compound K production from red ginseng root (C.A. Mayer) by sequential enzymatic hydrolysis and its characteristics

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Yeong-Ju Park ◽  
Unsik Hwang ◽  
Suyeon Park ◽  
Sol Sim ◽  
Soyeon Jeong ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Serum Glucose ◽  
Single Step ◽  
Enzyme Treatment ◽  
Heart Weight ◽  
Ginseng Root ◽  
Root Extract ◽  
Red Ginseng ◽  
Compound K

AbstractCompound K (CK; 20-O-β-(d-glucopyranosyl)-20(S)-protopanaxadiol) is one of the metabolites of ginsenosides contained in red ginseng (RG) and is known to have high bioavailability. This study aimed to establish the optimal conditions for enzyme treatment to convert ginsenosides from RG extract to CK, and to prove the characteristics of bioconverted red ginseng (BRG) extract. CK was not detected in unenzyme-treated RG extract, and in the single-step enzyme treatment, it was produced at less than 4.58 mg/g only in treatment group with Pyr-flo or Sumizyme AC (at 50 °C for 48 h). The highest yield of CK (14.32 mg/g) was obtained by Ultimase MFC treatment at 50 °C for 48 h after treatment with a mixture of Pyr-flo and Rapidase at 50 °C for 24 h. Total polyphenol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) (ABTS) radical scavenging activity were higher in BRG than in RG (p < 0.5). High-fat diet (HD) rat fed 1% BRG had significantly lower body weight, heart weight, fat pads (periosteal fat, epididymal fat), serum glucose levels, and hepatic triglyceride levels than those HD rat fed 1% RG (p < 0.05). In conclusion, the sequential enzymatic bioconversion was produces higher CK in RG root extract than single-step enzyme treatment.

scholarly journals Reduced Self-Perception of Fatigue after Intake of Panax ginseng Root Extract (G115®) Formulated with Vitamins and Minerals—An Open-Label Study

2021 ◽  
Vol 18 (12) ◽  
pp. 6257
Author(s):  
Anne-Laure Tardy ◽  
Beatrice Bois De Fer ◽  
Salvador Cañigueral ◽  
David Kennedy ◽  
Andrew Scholey ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Daily Intake ◽  
Energy Performance ◽  
Ginseng Root ◽  
Root Extract ◽  
Physical Fatigue ◽  
Self Perception ◽  
Open Label ◽  
Open Label Study ◽  
Label Study

Background: Unexplained fatigue is a common complaint. When underlying disease causes have been eliminated, lifestyle measures and supplementation can be indicated. Elaborating on clinical findings that G115®, a dry extract from the root of Panax ginseng, combined with vitamins and minerals could alleviate fatigue, this open label study aimed at assessing its effect on perceived fatigue and energy. Methods: Healthy adults self-reporting fatigue (n = 103) completed the Multidimensional Fatigue Inventory questionnaire. They rated their perceptions of mental and physical fatigue, energy, performance, and stress at baseline and 15, 30, 60 and 90 days after a daily intake of 40 mg G115® formulated with vitamins and minerals. Results: Compared with baseline values, mean self-perception of general fatigue was reduced by −7.55 units [95% CI: −8.44; −6.66] (−41.8%, p < 0.0001) at 90 days. All assessed perception ratings (mental and physical fatigue, reduced activity and motivation, performance, and stress) were significantly and steadily improved from two weeks after supplementation up to study’s end. Overall satisfaction with the ability of the product to reduce fatigue reached 85% at Day 90. Conclusion: Daily intake with G115® extract formulated with vitamins and minerals suggests an improvement of self-perception of fatigue and energy in a fatigued adult population.

scholarly journals Ginsenoside compound K induces apoptosis in nasopharyngeal carcinoma cells via activation of apoptosis-inducing factor

2014 ◽  
Vol 9 (1) ◽  
pp. 11 ◽  
Author(s):  
Carmen Ka-Man Law ◽  
Hoi-Hin Kwok ◽  
Po-Ying Poon ◽  
Chi-Chiu Lau ◽  
Zhi-Hong Jiang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Carcinoma Cells ◽  
Compound K ◽  
Ginsenoside Compound K ◽  
Apoptosis Inducing Factor

scholarly journals Production of bioactive ginsenoside compound K in metabolically engineered yeast

Cell Research ◽  
2014 ◽  
Vol 24 (6) ◽  
pp. 770-773 ◽  
Author(s):  
Xing Yan ◽  
Yun Fan ◽  
Wei Wei ◽  
Pingping Wang ◽  
Qunfang Liu ◽  
...  
Keyword(s):  
Compound K ◽  
Engineered Yeast ◽  
Ginsenoside Compound K

Ginsenoside compound K ameliorates Alzheimer’s disease in HT22 cells by adjusting energy metabolism

2019 ◽  
Vol 46 (5) ◽  
pp. 5323-5332 ◽  
Author(s):  
Xijun Chen ◽  
Hui Li ◽  
Qing Yang ◽  
Xingcheng Lan ◽  
Jifeng Wang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Energy Metabolism ◽  
Compound K ◽  
Ht22 Cells ◽  
Ginsenoside Compound K

scholarly journals Ginsenoside Compound K Regulates HIF-1α-Mediated Glycolysis Through Bclaf1 to Inhibit the Proliferation of Human Liver Cancer Cells

2020 ◽  
Vol 11 ◽  
Author(s):  
Silin Zhang ◽  
Meilan Zhang ◽  
Jiaxin Chen ◽  
Jiaqi Zhao ◽  
Jielin Su ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Hepatoma Cells ◽  
Dependent Manner ◽  
Compound K ◽  
Liver Cancer Cells ◽  
Hypoxic Conditions ◽  
Huh7 Cells ◽  
Glycolysis Pathway ◽  
Ginsenoside Compound K

This study aimed to demonstrate that ginsenoside compound K (20 (S)-ginsenoside CK; CK) downregulates Bcl-2-associated transcription factor 1 (Bclaf1), which inhibits the hypoxia-inducible factor-1α (HIF-1α)-mediated glycolysis pathway to inhibit the proliferation of liver cancer cells. Treatment of hepatoma cells (Bel-7404 and Huh7) under hypoxic conditions with different concentrations of CK showed that CK inhibited the proliferation of hepatoma cells in a time- and concentration-dependent manner; furthermore, the ability of the cells to form colonies was reduced, and cell growth was blocked in the G0/G1 phase. CK promoted the degradation of HIF-1α ubiquitination in liver cancer cells by regulating the expression of HIF-1α and related ubiquitination proteins; moreover, it reduced the activity of key enzymes involved in glycolysis, the pressure of cellular glycolysis, and the rate of real-time ATP production, thereby inhibiting the glycolysis pathway. It also decreased the expression of Bclaf1 in hypoxic liver cancer cells and thus reduced the ability of Bclaf1 to bind to HIF-1α. CK treatment of Bel-7404 and Huh7 cells with CRISPR/Cas9-engineered knock out of Bclaf1 gene under hypoxic conditions further suppressed the expression of HIF-1α, promoted HIF-1α ubiquitination, and inhibited the glycolysis pathway. In a rat model of primary liver cancer induced by diethylnitrosamine, positron emission tomography and computed tomography scans showed that after CK administration, tumor tissue volumes were reduced and glucose uptake capacity decreased. Increased Bclaf1 and HIF-1α expression promoted the ubiquitination of HIF-1α and inhibited the glycolysis pathway, thereby inhibiting the proliferation of liver cancer cells. In summary, this study confirmed by in vitro and in vivo experiments that in hypoxic liver cancer cells CK downregulates the expression of Bclaf1, inhibits the HIF-1α-mediated glycolysis pathway, and inhibits cell proliferation, suggesting that the CK-mediated effects on Bclaf1 may represent a novel therapeutic approach for the treatment of liver cancer patients.

Biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications

2015 ◽  
pp. 1-6 ◽  
Author(s):  
Priyanka Singh ◽  
Yeon Ju Kim ◽  
Chao Wang ◽  
Ramya Mathiyalagan ◽  
Mohamed El-Agamy Farh ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Ginseng Root ◽  
Root Extract ◽  
Red Ginseng ◽  
Silver And Gold Nanoparticles
Sign in / Sign up

Export Citation Format

Share Document