scholarly journals Improvement ofL-Lysine Production byMethylophilus methylotrophusfrom Methanolviathe Entner-Doudoroff Pathway, Originating inEscherichia coli

2008 ◽  
Vol 72 (10) ◽  
pp. 2535-2542 ◽  
Author(s):  
Kohei ISHIKAWA ◽  
Yoshiya GUNJI ◽  
Hisashi YASUEDA ◽  
Kozo ASANO
Keyword(s):  
Inescherichia Coli ◽  
Lysine Production

Expression of the NADP+-Dependent Formate Dehydrogenase Gene from Pseudomonas Increases the Lysine Production in Corynebacterium glutamicum

2019 ◽  
Vol 35 (6) ◽  
pp. 21-29
Author(s):  
T.E. Leonova ◽  
T.E. Shustikova ◽  
T.V. Gerasimova ◽  
Т.А. Ivankova ◽  
K.V. Sidorenko Sidorenko ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Formate Dehydrogenase ◽  
Ministry Of Education ◽  
Lysine Production ◽  
Simultaneous Formation ◽  
Resource Center ◽  
Formate Oxidation ◽  
Gene Coding ◽  
Dehydrogenase Gene ◽  
Lysine Synthesis

Thepsefdh_D221Q gene coding for a mutant formate dehydrogenase (PseFDG_D221Q) from Pseudomonas, which catalyzes the formate oxidation with the simultaneous formation of NADPH, has been expressed in the cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into С. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with simultaneous inactivation of host formate dehydrogenase genes. It was shown that the С. glutamicum strains with NADP+ -dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate, if their own formate dehydrogenase is inactivated. L-lysine, formate dehydrogenase, NADPH, Corynebacterium glutamicum The work was carried out using the equipment of the Multipurpose Scientific This work was carried out on the equipment of the Multipurpose Scientific Installation of «All-Russian Collection of Industrial Microorganisms», National Bio-Resource Center, NRC «Kurchatov Institute»- GosNIIgenetika. This work was financially supported by the Ministry of Education and Science of Russia (Unique Project Identifier - RFMEFI61017X0011).

scholarly journals Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation inEscherichia coli

2019 ◽  
Vol 116 (8) ◽  
pp. 2074-2086 ◽  
Author(s):  
Gavin Kurgan ◽  
Christian Sievert ◽  
Andrew Flores ◽  
Aidan Schneider ◽  
Thomas Billings ◽  
...  
Keyword(s):  
Experimental Evolution ◽  
Xylose Fermentation ◽  
Inescherichia Coli
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