Annexin A3-Expressing Cellular Phenotypes Emerge from Necrotic Lesion in the Pericentral Area in 2-Acetylaminofluoren/Carbon Tetrachloride-Treated Rat Livers

2007 ◽  
Vol 71 (12) ◽  
pp. 3082-3089 ◽  
Author(s):  
Yoshimasa ITO ◽  
Takenori WATANABE ◽  
Shunsuke NAGATOMO ◽  
Taiichiro SEKI ◽  
Shingo NIIMI ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Necrotic Lesion ◽  
Annexin A3 ◽  
Cellular Phenotypes ◽  
Rat Livers ◽  
Pericentral Area

LIVER CIRRHOSIS AND OESTROGEN METABOLISM – PERFUSION STUDIES WITH CIRRHOTIC RAT LIVERS

1979 ◽  
Vol 90 (4) ◽  
pp. 658-668
Author(s):  
Michael Höller ◽  
Karin Dengler ◽  
Renate Fabricius ◽  
Heinz Breuer
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Enterohepatic Circulation ◽  
Hepatic Uptake ◽  
Distribution Volume ◽  
List Type ◽  
Combined Application ◽  
Perfusion Studies ◽  
Rat Livers

ABSTRACT Radioactive oestrone and oestradiol-17β were perfused through normal and cirrhotic livers of rats. Liver cirrhosis had been induced by a combined application of carbon tetrachloride and azathioprine. The hepatic uptake of both oestrogens by cirrhotic livers was reduced; the uptake of oestrone was more affected than that of oestradiol-17β, The pattern of oestrogen metabolites indicated a reduction of the sulphotransferase activity in cirrhotic livers. The activity of other enzymes of oestrogen metabolism were similar in normal and cirrhotic livers. The amount of oestrogen glucuronides excreted into the bile was significantly less in cirrhotic livers although the bile volume was larger in cirrhotic than in normal livers. The release of oestrogen metabolites into the circulating medium was considerably higher during perfusion of cirrhotic livers. From the findings presented here it is concluded that the turnover of oestrogens is slower in cirrhotic than in normal livers. Moreover, it may be speculated that the distribution volume of the oestrogen metabolites is smaller in rats with liver cirrhosis, due to a disturbed enterohepatic circulation. This results in higher oestrogen concentrations in extracellular fluids, thus supporting the concept of hyper-oestrogenism in liver cirrhosis.

Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology

2003 ◽  
Vol 3 (1) ◽  
pp. 41-52 ◽  
Author(s):  
M B Young ◽  
M R DiSilvestro ◽  
T J Sendera ◽  
J Freund ◽  
A Kriete ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carbon Tetrachloride ◽  
Rat Livers

scholarly journals Salvianolic Acid B Reducing Portal Hypertension Depends on Macrophages in Isolated Portal Perfused Rat Livers with Chronic Hepatitis

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Xin Zhao ◽  
Hongmei Jia ◽  
Shijun Yang ◽  
Yuetao Liu ◽  
Bo Deng ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chronic Hepatitis ◽  
Portal Hypertension ◽  
Carbon Tetrachloride ◽  
Heme Oxygenase 1 ◽  
Perfused Rat Liver ◽  
Portal Veins ◽  
Oxide Synthase ◽  
Rat Livers ◽  
Inducible Nitric Oxide

This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl4) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed atd0,d28,d56, andd84in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC50of Sal B for relaxing portal veins was−2.04×10−9,7.28×10−11,1.52×10−11, and8.44×10−11 mol/L atd0,d28,d56, andd84, respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least.

scholarly journals QUANTITATIVE STUDY OF COLLAGEN CONTENT IN EXPERIMENTAL CIRRHOSIS

1947 ◽  
Vol 85 (3) ◽  
pp. 217-226 ◽  
Author(s):  
Thomas G. Morrione
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Cirrhosis ◽  
Normal Diet ◽  
Collagen Content ◽  
Hepatic Regeneration ◽  
Prolonged Administration ◽  
Experimental Cirrhosis ◽  
Fourfold Increase ◽  
Rat Livers ◽  
Partial Reversal

Quantitative determinations of collagen were carried out on rat livers showing cirrhosis due to p-dimethylaminoazobenzene and carbon tetrachloride. A twofold increase in collagen content occurred in cirrhosis due to p-dimethylaminoazobenzene. The average total hepatic collagen as well as the per cent collagen content were doubled. The collagen content after 1½ months of normal diet had fallen to normal levels. In cirrhosis due to carbon tetrachloride, the collagen content underwent about a fourfold increase. Partial reversal with significant decrease in collagen content occurred after stopping the carbon tetrachloride. The incomplete resorption of collagen in this group can be attributed to impaired hepatic regeneration following the prolonged administration of the compound. The chemical values for collagen parallel the quantitative evaluations of collagen content, based on microscopic examination of liver sections stained for reticulum. Deposition of collagen in hepatic cirrhosis is not necessarily an irreversible phenomenon.

Ultrastructural changes in isolated rat liver perfused with cyclic heptapeptide toxins from norwegian freshwater cyanobacteria (blue-green algae)

1987 ◽  
Vol 45 ◽  
pp. 858-859
Author(s):  
A.S. Dabholkar ◽  
W.W. Carmichael ◽  
K. Berg ◽  
J. Wyman
Keyword(s):  
Ultrastructural Changes ◽  
Sprague Dawley ◽  
Isolated Rat Liver ◽  
Blue Green Algae ◽  
Sprague Dawley Rats ◽  
Cyclic Heptapeptide ◽  
Intracellular Changes ◽  
Oscillatoria Agardhii ◽  
Rat Livers ◽  
Isolated Rat

Intracellular changes in the hepatocytes of isolated rat livers perfused with cyclic heptapeptide toxins are described. The toxins used are 1) -Ala-Leu- β-methyl isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 944) from Microcystis aeruginosa- Lake Akersvatn, Norway; 2) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 1023) from Oscillatoria agardhii var. - Lake Kolbatnvatn, Norway; 3) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-dha (M.W. 1009) from Oscillatoria agardhii var. isothrix - Lake Froylandsvatn, Norway. Approximate LD intraperitoneal mouse for the toxins is 50, 500 and 1000 μg/kg respectively.Livers were removed from male Sprague Dawley rats and perfused for 15 min with a blood-free perfusate (50 ml) followed by 60 min with perfusate containing i) 25, 50, or 200 μg of M. aeruginosa toxin ii) 50, 250, 500 or 1000 μg of O. agardhii var. toxin and iii) 1000, 2000, 2500 or 5000 μg of O. agardhii var. isothrix toxin. Control livers were perfused for 75 min with the blood-free perfusate.

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