scholarly journals Oxidative Dimer Produced from a 2,3,4-Trihydroxybenzoic Ester

2007 ◽  
Vol 71 (7) ◽  
pp. 1731-1734 ◽  
Author(s):  
Asuka KODAMA ◽  
Hidetoshi SHIBANO ◽  
Jun KAWABATA
Keyword(s):  
Oxidative Dimer

scholarly journals Genetic and Biochemical Analysis of Dimer and Oligomer Interactions of the λ S Holin

2000 ◽  
Vol 182 (21) ◽  
pp. 6082-6090 ◽  
Author(s):  
Angelika Gründling ◽  
Udo Bläsi ◽  
Ry Young
Keyword(s):  
Host Cell ◽  
Cell Lysis ◽  
Disulfide Bridge ◽  
Dominant Negative ◽  
Hydrophobic Core ◽  
Inner Membrane ◽  
S Protein ◽  
Bridge Formation ◽  
Oxidative Dimer ◽  
Membrane Spanning

ABSTRACT Bacteriophage λ uses a holin-endolysin system for host cell lysis. R, the endolysin, has muralytic activity. S, the holin, is a small membrane protein that permeabilizes the inner membrane at a precisely scheduled time after infection and allows the endolysin access to its substrate, resulting in host cell lysis. λ S has a single cysteine at position 51 that can be replaced by a serine without loss of the holin function. A collection of 27 single-cysteine products of alleles created from λ SC51S were tested for holin function. Most of the single-cysteine variants retained the ability to support lysis. Mutations with the most defective phenotype clustered in the first two hydrophobic transmembrane domains. Several lines of evidence indicate that S forms an oligomeric structure in the inner membrane. Here we show that oligomerization does not depend on disulfide bridge formation, since the cysteineless SC51S(i) is functional as a holin and (ii) shows the same oligomerization pattern as the parental S protein. In contrast, the lysis-defective SA52V mutant dimerizes but does not form cross-linkable oligomers. Again, dimerization does not depend on the natural cysteine, since the cysteineless lysis-defective SA52V/C51S is found in dimers after treatment of the membrane with a cross-linking agent. Furthermore, under oxidative conditions, dimerization via the natural cysteine is very efficient for SA52V. Both SA52V (dominant negative) and SA48V(antidominant) interact with the parental S protein, as judged by oxidative disulfide bridge formation. Thus, productive and unproductive heterodimer formation between the parental protein and the mutants SA52V and SA48V, respectively, may account for the dominant and antidominant lysis phenotypes. Examination of oxidative dimer formation between S variants with single cysteines in the hydrophobic core of the second membrane-spanning domain revealed that positions 48 and 51 are on a dimer interface. These results are discussed in terms of a three-step model leading to S-dependent hole formation in the inner membrane.

scholarly journals Oxidative formation of bis-N-methylquinolinone from anti-head-to-head N-methylquinolinone cyclodimer

2021 ◽  
Author(s):  
Nikolai Bieniek ◽  
Christian P. Haas ◽  
Ulrich Tallarek ◽  
Norbert Hampp
Keyword(s):  
Singlet Oxygen ◽  
Photochemical Reaction ◽  
Flow Reactor ◽  
Type Systems ◽  
Tube Flow ◽  
Pericyclic Reactions ◽  
Light Activation ◽  
Oxidative Conversion ◽  
Oxidative Dimer ◽  
Triggered Drug Release

AbstractThe light-driven formation and cleavage of cyclobutane structural motifs resulting from [2 + 2]-pericyclic reactions, as found in thymine and coumarin-type systems, is an important and intensively studied photochemical reaction. Various applications are reported utilizing these systems, among others, in cross-linked polymers, light-triggered drug release, or other technical applications. Herein coumarin is most frequently used as the photoactive group. Quite often, a poor quantum yield for dimerization and cyclobutane-cleavage and a lack of reversibility are described. In this work, we present the identification of a heterogeneous pathway of dimer cleavage found in a rarely studied coumarin analog molecule, the N-methyl-quinolinone (NMQ). The monomer was irradiated in a tube flow-reactor and the reaction process was monitored using online HPLC measurements. We found the formation of a pseudo-equilibrium between monomeric and dimeric NMQ and a continuous rise of a side product via oxidative dimer splitting and proton elimination which was identified as 3,3’-bis-NMQ. Oxidative conversion by singlet oxygen was identified to be the cause of this non-conventional cyclobutane cleavage. The addition of antioxidants suppressing singlet oxygen enables achieving a 100% photochemical conversion from NMQ to the anti-head-to-head-NMQ-dimer. Using dissolved oxygen upon light activation to singlet oxygen limits the reversibility of the photochemical [2 + 2]-cycloaddition and cycloreversion of NMQ and most likely comparable systems. Based on these findings, the development of highly efficient cycloaddition–cycloreversion systems should be enabled.

Novel Oxidative Dimer from Caffeic Acid

2003 ◽  
Vol 67 (5) ◽  
pp. 1185-1187 ◽  
Author(s):  
Hiroyuki TAZAKI ◽  
Jun KAWABATA ◽  
Takashi FUJITA
Keyword(s):  
Caffeic Acid ◽  
Oxidative Dimer

Oxidative Dimer Formation Is the Critical Rate-Limiting Step for Parkinson's Disease α-Synuclein Fibrillogenesis†

Biochemistry ◽  
2003 ◽  
Vol 42 (3) ◽  
pp. 829-837 ◽  
Author(s):  
Sampathkumar Krishnan ◽  
Eva Y. Chi ◽  
Stephen J. Wood ◽  
Brent S. Kendrick ◽  
Cynthia Li ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Dimer Formation ◽  
Critical Rate ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Oxidative Dimer ◽  
Rate Limiting
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